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Real-time RT-PCR high-resolution melting curve analysis and multiplex RT-PCR to detect and differentiate grapevine leafroll-associated virus 3 variant groups I, II, III and VI

BACKGROUND: Grapevine leafroll-associated virus 3 (GLRaV-3) is the main contributing agent of leafroll disease worldwide. Four of the six GLRaV-3 variant groups known have been found in South Africa, but their individual contribution to leafroll disease is unknown. In order to study the pathogenesis...

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Detalles Bibliográficos
Autores principales: Bester, Rachelle, Jooste, Anna E C, Maree, Hans J, Burger, Johan T
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3519601/
https://www.ncbi.nlm.nih.gov/pubmed/23016734
http://dx.doi.org/10.1186/1743-422X-9-219