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mMaple: A Photoconvertible Fluorescent Protein for Use in Multiple Imaging Modalities
Recent advances in fluorescence microscopy have extended the spatial resolution to the nanometer scale. Here, we report an engineered photoconvertible fluorescent protein (pcFP) variant, designated as mMaple, that is suited for use in multiple conventional and super-resolution imaging modalities, sp...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3519878/ https://www.ncbi.nlm.nih.gov/pubmed/23240015 http://dx.doi.org/10.1371/journal.pone.0051314 |
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author | McEvoy, Ann L. Hoi, Hiofan Bates, Mark Platonova, Evgenia Cranfill, Paula J. Baird, Michelle A. Davidson, Michael W. Ewers, Helge Liphardt, Jan Campbell, Robert E. |
author_facet | McEvoy, Ann L. Hoi, Hiofan Bates, Mark Platonova, Evgenia Cranfill, Paula J. Baird, Michelle A. Davidson, Michael W. Ewers, Helge Liphardt, Jan Campbell, Robert E. |
author_sort | McEvoy, Ann L. |
collection | PubMed |
description | Recent advances in fluorescence microscopy have extended the spatial resolution to the nanometer scale. Here, we report an engineered photoconvertible fluorescent protein (pcFP) variant, designated as mMaple, that is suited for use in multiple conventional and super-resolution imaging modalities, specifically, widefield and confocal microscopy, structured illumination microscopy (SIM), and single-molecule localization microscopy. We demonstrate the versatility of mMaple by obtaining super-resolution images of protein organization in Escherichia coli and conventional fluorescence images of mammalian cells. Beneficial features of mMaple include high photostability of the green state when expressed in mammalian cells and high steady state intracellular protein concentration of functional protein when expressed in E. coli. mMaple thus enables both fast live-cell ensemble imaging and high precision single molecule localization for a single pcFP-containing construct. |
format | Online Article Text |
id | pubmed-3519878 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-35198782012-12-13 mMaple: A Photoconvertible Fluorescent Protein for Use in Multiple Imaging Modalities McEvoy, Ann L. Hoi, Hiofan Bates, Mark Platonova, Evgenia Cranfill, Paula J. Baird, Michelle A. Davidson, Michael W. Ewers, Helge Liphardt, Jan Campbell, Robert E. PLoS One Research Article Recent advances in fluorescence microscopy have extended the spatial resolution to the nanometer scale. Here, we report an engineered photoconvertible fluorescent protein (pcFP) variant, designated as mMaple, that is suited for use in multiple conventional and super-resolution imaging modalities, specifically, widefield and confocal microscopy, structured illumination microscopy (SIM), and single-molecule localization microscopy. We demonstrate the versatility of mMaple by obtaining super-resolution images of protein organization in Escherichia coli and conventional fluorescence images of mammalian cells. Beneficial features of mMaple include high photostability of the green state when expressed in mammalian cells and high steady state intracellular protein concentration of functional protein when expressed in E. coli. mMaple thus enables both fast live-cell ensemble imaging and high precision single molecule localization for a single pcFP-containing construct. Public Library of Science 2012-12-11 /pmc/articles/PMC3519878/ /pubmed/23240015 http://dx.doi.org/10.1371/journal.pone.0051314 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. |
spellingShingle | Research Article McEvoy, Ann L. Hoi, Hiofan Bates, Mark Platonova, Evgenia Cranfill, Paula J. Baird, Michelle A. Davidson, Michael W. Ewers, Helge Liphardt, Jan Campbell, Robert E. mMaple: A Photoconvertible Fluorescent Protein for Use in Multiple Imaging Modalities |
title | mMaple: A Photoconvertible Fluorescent Protein for Use in Multiple Imaging Modalities |
title_full | mMaple: A Photoconvertible Fluorescent Protein for Use in Multiple Imaging Modalities |
title_fullStr | mMaple: A Photoconvertible Fluorescent Protein for Use in Multiple Imaging Modalities |
title_full_unstemmed | mMaple: A Photoconvertible Fluorescent Protein for Use in Multiple Imaging Modalities |
title_short | mMaple: A Photoconvertible Fluorescent Protein for Use in Multiple Imaging Modalities |
title_sort | mmaple: a photoconvertible fluorescent protein for use in multiple imaging modalities |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3519878/ https://www.ncbi.nlm.nih.gov/pubmed/23240015 http://dx.doi.org/10.1371/journal.pone.0051314 |
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