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Spontaneous Production of Immunoglobulin M in Human Epithelial Cancer Cells
It is well known that B-1 B cells are the main cell type that is responsible for the production of natural immunoglobulin M (IgM) and can respond to infection by increasing IgM secretion. However, we unexpectedly found that some epithelial cells also can express rearranged IgM transcript that has na...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3520907/ https://www.ncbi.nlm.nih.gov/pubmed/23251529 http://dx.doi.org/10.1371/journal.pone.0051423 |
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author | Hu, Fanlei Zhang, Li Zheng, Jie Zhao, Ling Huang, Jing Shao, Wenwei Liao, Qinyuan Ma, Teng Geng, Li Yin, C. Cameron Qiu, Xiaoyan |
author_facet | Hu, Fanlei Zhang, Li Zheng, Jie Zhao, Ling Huang, Jing Shao, Wenwei Liao, Qinyuan Ma, Teng Geng, Li Yin, C. Cameron Qiu, Xiaoyan |
author_sort | Hu, Fanlei |
collection | PubMed |
description | It is well known that B-1 B cells are the main cell type that is responsible for the production of natural immunoglobulin M (IgM) and can respond to infection by increasing IgM secretion. However, we unexpectedly found that some epithelial cells also can express rearranged IgM transcript that has natural IgM characteristics, such as germline-encoded and restricted rearrangement patterns. Here we studied IgM expression in human non-B cells and found that IgM was frequently expressed by many human epithelial cancer cells as well as non-cancer epithelial cells. Moreover, CD79A and CD79B, two molecules that are physically linked to membranous IgM on the surface of B cells to form the B cell antigen receptor complex, were also expressed on the cell surface of epithelial cancer cells and co-located with IgM. Like the natural IgM, the epithelial cancer cell-derived IgM recognized a series of microbial antigens, such as single-stranded DNA, double-stranded DNA, lipopolysaccharide, and the HEp-2 cell antigen. More important, stimulation of the toll-like receptor 9 (TLR9), which mimics bacterial infection, substantially increased the secretion of IgM in human epithelial cancer cells. These findings indicate that human epithelial cancer cells as well as non-cancer epithelial cells can spontaneously produce IgM with natural antibody activity. |
format | Online Article Text |
id | pubmed-3520907 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-35209072012-12-18 Spontaneous Production of Immunoglobulin M in Human Epithelial Cancer Cells Hu, Fanlei Zhang, Li Zheng, Jie Zhao, Ling Huang, Jing Shao, Wenwei Liao, Qinyuan Ma, Teng Geng, Li Yin, C. Cameron Qiu, Xiaoyan PLoS One Research Article It is well known that B-1 B cells are the main cell type that is responsible for the production of natural immunoglobulin M (IgM) and can respond to infection by increasing IgM secretion. However, we unexpectedly found that some epithelial cells also can express rearranged IgM transcript that has natural IgM characteristics, such as germline-encoded and restricted rearrangement patterns. Here we studied IgM expression in human non-B cells and found that IgM was frequently expressed by many human epithelial cancer cells as well as non-cancer epithelial cells. Moreover, CD79A and CD79B, two molecules that are physically linked to membranous IgM on the surface of B cells to form the B cell antigen receptor complex, were also expressed on the cell surface of epithelial cancer cells and co-located with IgM. Like the natural IgM, the epithelial cancer cell-derived IgM recognized a series of microbial antigens, such as single-stranded DNA, double-stranded DNA, lipopolysaccharide, and the HEp-2 cell antigen. More important, stimulation of the toll-like receptor 9 (TLR9), which mimics bacterial infection, substantially increased the secretion of IgM in human epithelial cancer cells. These findings indicate that human epithelial cancer cells as well as non-cancer epithelial cells can spontaneously produce IgM with natural antibody activity. Public Library of Science 2012-12-12 /pmc/articles/PMC3520907/ /pubmed/23251529 http://dx.doi.org/10.1371/journal.pone.0051423 Text en © 2012 Hu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Hu, Fanlei Zhang, Li Zheng, Jie Zhao, Ling Huang, Jing Shao, Wenwei Liao, Qinyuan Ma, Teng Geng, Li Yin, C. Cameron Qiu, Xiaoyan Spontaneous Production of Immunoglobulin M in Human Epithelial Cancer Cells |
title | Spontaneous Production of Immunoglobulin M in Human Epithelial Cancer Cells |
title_full | Spontaneous Production of Immunoglobulin M in Human Epithelial Cancer Cells |
title_fullStr | Spontaneous Production of Immunoglobulin M in Human Epithelial Cancer Cells |
title_full_unstemmed | Spontaneous Production of Immunoglobulin M in Human Epithelial Cancer Cells |
title_short | Spontaneous Production of Immunoglobulin M in Human Epithelial Cancer Cells |
title_sort | spontaneous production of immunoglobulin m in human epithelial cancer cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3520907/ https://www.ncbi.nlm.nih.gov/pubmed/23251529 http://dx.doi.org/10.1371/journal.pone.0051423 |
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