Enzyme activity assays within microstructured optical fibers enabled by automated alignment

A fluorescence-based enzyme activity assay has been demonstrated within a small-core microstructured optical fiber (MOF) for the first time. To achieve this, a reflection-based automated alignment system has been developed, which uses feedback and piezoelectric actuators to maintain optical alignmen...

Descripción completa

Detalles Bibliográficos
Autores principales: Warren-Smith, Stephen C., Nie, Guiying, Schartner, Erik P., Salamonsen, Lois A., Monro, Tanya M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2012
Materias:
Biosensors and Molecular Diagnostics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3521297/
https://www.ncbi.nlm.nih.gov/pubmed/23243579
http://dx.doi.org/10.1364/BOE.3.003304
Descripción
Sumario:A fluorescence-based enzyme activity assay has been demonstrated within a small-core microstructured optical fiber (MOF) for the first time. To achieve this, a reflection-based automated alignment system has been developed, which uses feedback and piezoelectric actuators to maintain optical alignment. The auto-alignment system provides optical stability for the time required to perform an activity assay. The chosen assay is based on the enzyme proprotein convertase 5/6 (PC6) and has important applications in women’s health.

Ejemplares similares