Enzyme activity assays within microstructured optical fibers enabled by automated alignment

A fluorescence-based enzyme activity assay has been demonstrated within a small-core microstructured optical fiber (MOF) for the first time. To achieve this, a reflection-based automated alignment system has been developed, which uses feedback and piezoelectric actuators to maintain optical alignmen...

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Detalles Bibliográficos
Autores principales: Warren-Smith, Stephen C., Nie, Guiying, Schartner, Erik P., Salamonsen, Lois A., Monro, Tanya M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2012
Materias:
Biosensors and Molecular Diagnostics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3521297/
https://www.ncbi.nlm.nih.gov/pubmed/23243579
http://dx.doi.org/10.1364/BOE.3.003304
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author Warren-Smith, Stephen C.
Nie, Guiying
Schartner, Erik P.
Salamonsen, Lois A.
Monro, Tanya M.
author_facet Warren-Smith, Stephen C.
Nie, Guiying
Schartner, Erik P.
Salamonsen, Lois A.
Monro, Tanya M.
author_sort Warren-Smith, Stephen C.
collection PubMed
description A fluorescence-based enzyme activity assay has been demonstrated within a small-core microstructured optical fiber (MOF) for the first time. To achieve this, a reflection-based automated alignment system has been developed, which uses feedback and piezoelectric actuators to maintain optical alignment. The auto-alignment system provides optical stability for the time required to perform an activity assay. The chosen assay is based on the enzyme proprotein convertase 5/6 (PC6) and has important applications in women’s health.
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spelling pubmed-35212972012-12-14 Enzyme activity assays within microstructured optical fibers enabled by automated alignment Warren-Smith, Stephen C. Nie, Guiying Schartner, Erik P. Salamonsen, Lois A. Monro, Tanya M. Biomed Opt Express Biosensors and Molecular Diagnostics A fluorescence-based enzyme activity assay has been demonstrated within a small-core microstructured optical fiber (MOF) for the first time. To achieve this, a reflection-based automated alignment system has been developed, which uses feedback and piezoelectric actuators to maintain optical alignment. The auto-alignment system provides optical stability for the time required to perform an activity assay. The chosen assay is based on the enzyme proprotein convertase 5/6 (PC6) and has important applications in women’s health. Optical Society of America 2012-11-20 /pmc/articles/PMC3521297/ /pubmed/23243579 http://dx.doi.org/10.1364/BOE.3.003304 Text en ©2012 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.
spellingShingle Biosensors and Molecular Diagnostics
Warren-Smith, Stephen C.
Nie, Guiying
Schartner, Erik P.
Salamonsen, Lois A.
Monro, Tanya M.
Enzyme activity assays within microstructured optical fibers enabled by automated alignment
title Enzyme activity assays within microstructured optical fibers enabled by automated alignment
title_full Enzyme activity assays within microstructured optical fibers enabled by automated alignment
title_fullStr Enzyme activity assays within microstructured optical fibers enabled by automated alignment
title_full_unstemmed Enzyme activity assays within microstructured optical fibers enabled by automated alignment
title_short Enzyme activity assays within microstructured optical fibers enabled by automated alignment
title_sort enzyme activity assays within microstructured optical fibers enabled by automated alignment
topic Biosensors and Molecular Diagnostics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3521297/
https://www.ncbi.nlm.nih.gov/pubmed/23243579
http://dx.doi.org/10.1364/BOE.3.003304
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