Surface plasmon resonance as detection tool for lipids lateral mobility in biomimetic membranes

A procedure based on surface plasmon resonance (SPR) is proposed to monitor the lateral mobility of lipid molecules in solid-supported bilayer lipid membranes (ssBLMs), an essential prerequisite for the formation of important microdomains called lipid rafts (LRs). The procedure relies on the marked...

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Autores principales: Margheri, Giancarlo, D’Agostino, Riccardo, Becucci, Lucia, Guidelli, Rolando, Tiribilli, Bruno, Del Rosso, Mario
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2012
Materias:
Optical Biophysics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3521305/
https://www.ncbi.nlm.nih.gov/pubmed/23243563
http://dx.doi.org/10.1364/BOE.3.003119
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author Margheri, Giancarlo
D’Agostino, Riccardo
Becucci, Lucia
Guidelli, Rolando
Tiribilli, Bruno
Del Rosso, Mario
author_facet Margheri, Giancarlo
D’Agostino, Riccardo
Becucci, Lucia
Guidelli, Rolando
Tiribilli, Bruno
Del Rosso, Mario
author_sort Margheri, Giancarlo
collection PubMed
description A procedure based on surface plasmon resonance (SPR) is proposed to monitor the lateral mobility of lipid molecules in solid-supported bilayer lipid membranes (ssBLMs), an essential prerequisite for the formation of important microdomains called lipid rafts (LRs). The procedure relies on the marked tendency of the ganglioside GM1 to be recruited by LRs and to act as a specific receptor of the beta-subunit of the cholera toxin (ChTB). In the presence of both GM1 and ChTB, spontaneous formation of lipid rafts domains in mobile ssBLMs is accompanied by an appreciable increase in the amount of adsorbed ChTB, as monitored by SPR.
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spelling pubmed-35213052012-12-14 Surface plasmon resonance as detection tool for lipids lateral mobility in biomimetic membranes Margheri, Giancarlo D’Agostino, Riccardo Becucci, Lucia Guidelli, Rolando Tiribilli, Bruno Del Rosso, Mario Biomed Opt Express Optical Biophysics A procedure based on surface plasmon resonance (SPR) is proposed to monitor the lateral mobility of lipid molecules in solid-supported bilayer lipid membranes (ssBLMs), an essential prerequisite for the formation of important microdomains called lipid rafts (LRs). The procedure relies on the marked tendency of the ganglioside GM1 to be recruited by LRs and to act as a specific receptor of the beta-subunit of the cholera toxin (ChTB). In the presence of both GM1 and ChTB, spontaneous formation of lipid rafts domains in mobile ssBLMs is accompanied by an appreciable increase in the amount of adsorbed ChTB, as monitored by SPR. Optical Society of America 2012-11-06 /pmc/articles/PMC3521305/ /pubmed/23243563 http://dx.doi.org/10.1364/BOE.3.003119 Text en ©2012 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.
spellingShingle Optical Biophysics
Margheri, Giancarlo
D’Agostino, Riccardo
Becucci, Lucia
Guidelli, Rolando
Tiribilli, Bruno
Del Rosso, Mario
Surface plasmon resonance as detection tool for lipids lateral mobility in biomimetic membranes
title Surface plasmon resonance as detection tool for lipids lateral mobility in biomimetic membranes
title_full Surface plasmon resonance as detection tool for lipids lateral mobility in biomimetic membranes
title_fullStr Surface plasmon resonance as detection tool for lipids lateral mobility in biomimetic membranes
title_full_unstemmed Surface plasmon resonance as detection tool for lipids lateral mobility in biomimetic membranes
title_short Surface plasmon resonance as detection tool for lipids lateral mobility in biomimetic membranes
title_sort surface plasmon resonance as detection tool for lipids lateral mobility in biomimetic membranes
topic Optical Biophysics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3521305/
https://www.ncbi.nlm.nih.gov/pubmed/23243563
http://dx.doi.org/10.1364/BOE.3.003119
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