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Whole-genome sequencing and identification of Morganella morganii KT pathogenicity-related genes

BACKGROUND: The opportunistic enterobacterium, Morganella morganii, which can cause bacteraemia, is the ninth most prevalent cause of clinical infections in patients at Changhua Christian Hospital, Taiwan. The KT strain of M. morganii was isolated during postoperative care of a cancer patient with a...

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Autores principales: Chen, Yu-Tin, Peng, Hwei-Ling, Shia, Wei-Chung, Hsu, Fang-Rong, Ken, Chuian-Fu, Tsao, Yu-Ming, Chen, Chang-Hua, Liu, Chun-Eng, Hsieh, Ming-Feng, Chen, Huang-Chi, Tang, Chuan-Yi, Ku, Tien-Hsiung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3521468/
https://www.ncbi.nlm.nih.gov/pubmed/23282187
http://dx.doi.org/10.1186/1471-2164-13-S7-S4
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author Chen, Yu-Tin
Peng, Hwei-Ling
Shia, Wei-Chung
Hsu, Fang-Rong
Ken, Chuian-Fu
Tsao, Yu-Ming
Chen, Chang-Hua
Liu, Chun-Eng
Hsieh, Ming-Feng
Chen, Huang-Chi
Tang, Chuan-Yi
Ku, Tien-Hsiung
author_facet Chen, Yu-Tin
Peng, Hwei-Ling
Shia, Wei-Chung
Hsu, Fang-Rong
Ken, Chuian-Fu
Tsao, Yu-Ming
Chen, Chang-Hua
Liu, Chun-Eng
Hsieh, Ming-Feng
Chen, Huang-Chi
Tang, Chuan-Yi
Ku, Tien-Hsiung
author_sort Chen, Yu-Tin
collection PubMed
description BACKGROUND: The opportunistic enterobacterium, Morganella morganii, which can cause bacteraemia, is the ninth most prevalent cause of clinical infections in patients at Changhua Christian Hospital, Taiwan. The KT strain of M. morganii was isolated during postoperative care of a cancer patient with a gallbladder stone who developed sepsis caused by bacteraemia. M. morganii is sometimes encountered in nosocomial settings and has been causally linked to catheter-associated bacteriuria, complex infections of the urinary and/or hepatobiliary tracts, wound infection, and septicaemia. M. morganii infection is associated with a high mortality rate, although most patients respond well to appropriate antibiotic therapy. To obtain insights into the genome biology of M. morganii and the mechanisms underlying its pathogenicity, we used Illumina technology to sequence the genome of the KT strain and compared its sequence with the genome sequences of related bacteria. RESULTS: The 3,826,919-bp sequence contained in 58 contigs has a GC content of 51.15% and includes 3,565 protein-coding sequences, 72 tRNA genes, and 10 rRNA genes. The pathogenicity-related genes encode determinants of drug resistance, fimbrial adhesins, an IgA protease, haemolysins, ureases, and insecticidal and apoptotic toxins as well as proteins found in flagellae, the iron acquisition system, a type-3 secretion system (T3SS), and several two-component systems. Comparison with 14 genome sequences from other members of Enterobacteriaceae revealed different degrees of similarity to several systems found in M. morganii. The most striking similarities were found in the IS4 family of transposases, insecticidal toxins, T3SS components, and proteins required for ethanolamine use (eut operon) and cobalamin (vitamin B(12)) biosynthesis. The eut operon and the gene cluster for cobalamin biosynthesis are not present in the other Proteeae genomes analysed. Moreover, organisation of the 19 genes of the eut operon differs from that found in the other non-Proteeae enterobacterial genomes. CONCLUSIONS: This is the first genome sequence of M. morganii, which is a clinically relevant pathogen. Comparative genome analysis revealed several pathogenicity-related genes and novel genes not found in the genomes of other members of Proteeae. Thus, the genome sequence of M. morganii provides important information concerning virulence and determinants of fitness in this pathogen.
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spelling pubmed-35214682012-12-14 Whole-genome sequencing and identification of Morganella morganii KT pathogenicity-related genes Chen, Yu-Tin Peng, Hwei-Ling Shia, Wei-Chung Hsu, Fang-Rong Ken, Chuian-Fu Tsao, Yu-Ming Chen, Chang-Hua Liu, Chun-Eng Hsieh, Ming-Feng Chen, Huang-Chi Tang, Chuan-Yi Ku, Tien-Hsiung BMC Genomics Proceedings BACKGROUND: The opportunistic enterobacterium, Morganella morganii, which can cause bacteraemia, is the ninth most prevalent cause of clinical infections in patients at Changhua Christian Hospital, Taiwan. The KT strain of M. morganii was isolated during postoperative care of a cancer patient with a gallbladder stone who developed sepsis caused by bacteraemia. M. morganii is sometimes encountered in nosocomial settings and has been causally linked to catheter-associated bacteriuria, complex infections of the urinary and/or hepatobiliary tracts, wound infection, and septicaemia. M. morganii infection is associated with a high mortality rate, although most patients respond well to appropriate antibiotic therapy. To obtain insights into the genome biology of M. morganii and the mechanisms underlying its pathogenicity, we used Illumina technology to sequence the genome of the KT strain and compared its sequence with the genome sequences of related bacteria. RESULTS: The 3,826,919-bp sequence contained in 58 contigs has a GC content of 51.15% and includes 3,565 protein-coding sequences, 72 tRNA genes, and 10 rRNA genes. The pathogenicity-related genes encode determinants of drug resistance, fimbrial adhesins, an IgA protease, haemolysins, ureases, and insecticidal and apoptotic toxins as well as proteins found in flagellae, the iron acquisition system, a type-3 secretion system (T3SS), and several two-component systems. Comparison with 14 genome sequences from other members of Enterobacteriaceae revealed different degrees of similarity to several systems found in M. morganii. The most striking similarities were found in the IS4 family of transposases, insecticidal toxins, T3SS components, and proteins required for ethanolamine use (eut operon) and cobalamin (vitamin B(12)) biosynthesis. The eut operon and the gene cluster for cobalamin biosynthesis are not present in the other Proteeae genomes analysed. Moreover, organisation of the 19 genes of the eut operon differs from that found in the other non-Proteeae enterobacterial genomes. CONCLUSIONS: This is the first genome sequence of M. morganii, which is a clinically relevant pathogen. Comparative genome analysis revealed several pathogenicity-related genes and novel genes not found in the genomes of other members of Proteeae. Thus, the genome sequence of M. morganii provides important information concerning virulence and determinants of fitness in this pathogen. BioMed Central 2012-12-07 /pmc/articles/PMC3521468/ /pubmed/23282187 http://dx.doi.org/10.1186/1471-2164-13-S7-S4 Text en Copyright ©2012 Chen et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Proceedings
Chen, Yu-Tin
Peng, Hwei-Ling
Shia, Wei-Chung
Hsu, Fang-Rong
Ken, Chuian-Fu
Tsao, Yu-Ming
Chen, Chang-Hua
Liu, Chun-Eng
Hsieh, Ming-Feng
Chen, Huang-Chi
Tang, Chuan-Yi
Ku, Tien-Hsiung
Whole-genome sequencing and identification of Morganella morganii KT pathogenicity-related genes
title Whole-genome sequencing and identification of Morganella morganii KT pathogenicity-related genes
title_full Whole-genome sequencing and identification of Morganella morganii KT pathogenicity-related genes
title_fullStr Whole-genome sequencing and identification of Morganella morganii KT pathogenicity-related genes
title_full_unstemmed Whole-genome sequencing and identification of Morganella morganii KT pathogenicity-related genes
title_short Whole-genome sequencing and identification of Morganella morganii KT pathogenicity-related genes
title_sort whole-genome sequencing and identification of morganella morganii kt pathogenicity-related genes
topic Proceedings
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3521468/
https://www.ncbi.nlm.nih.gov/pubmed/23282187
http://dx.doi.org/10.1186/1471-2164-13-S7-S4
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