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Conditioned Medium from Adipose Tissue-Derived Mesenchymal Stem Cells Induces CD4+FOXP3+ Cells and Increases IL-10 Secretion

Mesenchymal stem cells (MSCs) are a new and promising tool for therapy of autoimmune disorders. In recent years their possibility to take part in the modulation of the immune response is discussed. The exact mechanisms for immunoregulation realized by MSCs are not clear yet, but interactions with ot...

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Autores principales: Ivanova-Todorova, Ekaterina, Bochev, Ivan, Dimitrov, Rumen, Belemezova, Kalina, Mourdjeva, Milena, Kyurkchiev, Stanimir, Kinov, Plamen, Altankova, Iskra, Kyurkchiev, Dobroslav
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3521492/
https://www.ncbi.nlm.nih.gov/pubmed/23251077
http://dx.doi.org/10.1155/2012/295167
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author Ivanova-Todorova, Ekaterina
Bochev, Ivan
Dimitrov, Rumen
Belemezova, Kalina
Mourdjeva, Milena
Kyurkchiev, Stanimir
Kinov, Plamen
Altankova, Iskra
Kyurkchiev, Dobroslav
author_facet Ivanova-Todorova, Ekaterina
Bochev, Ivan
Dimitrov, Rumen
Belemezova, Kalina
Mourdjeva, Milena
Kyurkchiev, Stanimir
Kinov, Plamen
Altankova, Iskra
Kyurkchiev, Dobroslav
author_sort Ivanova-Todorova, Ekaterina
collection PubMed
description Mesenchymal stem cells (MSCs) are a new and promising tool for therapy of autoimmune disorders. In recent years their possibility to take part in the modulation of the immune response is discussed. The exact mechanisms for immunoregulation realized by MSCs are not clear yet, but interactions with other immunoregulatory cells may be involved in this process. The investigation of the influence of MSCs on the expression of FoxP3 and cytokine secretion by T helper cells was the aim of this study. T helper cells were isolated from PBMCs by magnetic separation and MSCs were isolated from human adipose tissue, and CD4+ T cells were cultured with conditional medium of MSCs. The methods which were used include flow cytometry, ELISA, and Human Proteome profiler kits. The results demonstrated that secretory factors in MSCs conditional medium lead to increased expression of FoxP3 and increased secretion of IL-10 by T helpers. The obtained results give us opportunity to discuss the interaction between two kinds of immunoregulatory cells: MSCs and FoxP3+ T helpers. We suppose that this interaction leads to increased number of immunosuppressive helpers which secrete IL-10. MSCs provide some of their immunosuppressive functions acting on T regulatory cells, and we believe that IL-6 secreted by MSCs is involved in this process.
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spelling pubmed-35214922012-12-18 Conditioned Medium from Adipose Tissue-Derived Mesenchymal Stem Cells Induces CD4+FOXP3+ Cells and Increases IL-10 Secretion Ivanova-Todorova, Ekaterina Bochev, Ivan Dimitrov, Rumen Belemezova, Kalina Mourdjeva, Milena Kyurkchiev, Stanimir Kinov, Plamen Altankova, Iskra Kyurkchiev, Dobroslav J Biomed Biotechnol Research Article Mesenchymal stem cells (MSCs) are a new and promising tool for therapy of autoimmune disorders. In recent years their possibility to take part in the modulation of the immune response is discussed. The exact mechanisms for immunoregulation realized by MSCs are not clear yet, but interactions with other immunoregulatory cells may be involved in this process. The investigation of the influence of MSCs on the expression of FoxP3 and cytokine secretion by T helper cells was the aim of this study. T helper cells were isolated from PBMCs by magnetic separation and MSCs were isolated from human adipose tissue, and CD4+ T cells were cultured with conditional medium of MSCs. The methods which were used include flow cytometry, ELISA, and Human Proteome profiler kits. The results demonstrated that secretory factors in MSCs conditional medium lead to increased expression of FoxP3 and increased secretion of IL-10 by T helpers. The obtained results give us opportunity to discuss the interaction between two kinds of immunoregulatory cells: MSCs and FoxP3+ T helpers. We suppose that this interaction leads to increased number of immunosuppressive helpers which secrete IL-10. MSCs provide some of their immunosuppressive functions acting on T regulatory cells, and we believe that IL-6 secreted by MSCs is involved in this process. Hindawi Publishing Corporation 2012 2012-11-20 /pmc/articles/PMC3521492/ /pubmed/23251077 http://dx.doi.org/10.1155/2012/295167 Text en Copyright © 2012 Ekaterina Ivanova-Todorova et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ivanova-Todorova, Ekaterina
Bochev, Ivan
Dimitrov, Rumen
Belemezova, Kalina
Mourdjeva, Milena
Kyurkchiev, Stanimir
Kinov, Plamen
Altankova, Iskra
Kyurkchiev, Dobroslav
Conditioned Medium from Adipose Tissue-Derived Mesenchymal Stem Cells Induces CD4+FOXP3+ Cells and Increases IL-10 Secretion
title Conditioned Medium from Adipose Tissue-Derived Mesenchymal Stem Cells Induces CD4+FOXP3+ Cells and Increases IL-10 Secretion
title_full Conditioned Medium from Adipose Tissue-Derived Mesenchymal Stem Cells Induces CD4+FOXP3+ Cells and Increases IL-10 Secretion
title_fullStr Conditioned Medium from Adipose Tissue-Derived Mesenchymal Stem Cells Induces CD4+FOXP3+ Cells and Increases IL-10 Secretion
title_full_unstemmed Conditioned Medium from Adipose Tissue-Derived Mesenchymal Stem Cells Induces CD4+FOXP3+ Cells and Increases IL-10 Secretion
title_short Conditioned Medium from Adipose Tissue-Derived Mesenchymal Stem Cells Induces CD4+FOXP3+ Cells and Increases IL-10 Secretion
title_sort conditioned medium from adipose tissue-derived mesenchymal stem cells induces cd4+foxp3+ cells and increases il-10 secretion
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3521492/
https://www.ncbi.nlm.nih.gov/pubmed/23251077
http://dx.doi.org/10.1155/2012/295167
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