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Histone Deacetylase HDA6 Is Functionally Associated with AS1 in Repression of KNOX Genes in Arabidopsis

ASYMMETRIC LEAVES 1 (AS1) is a MYB-type transcription repressor that controls leaf development by regulating KNOX gene expression, but the underlying molecular mechanism is still unclear. In this study, we demonstrated that AS1 can interact with the histone deacetylase HDA6 in vitro and in vivo. The...

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Autores principales: Luo, Ming, Yu, Chun-Wei, Chen, Fang-Fang, Zhao, Linmao, Tian, Gang, Liu, Xuncheng, Cui, Yuhai, Yang, Jun-Yi, Wu, Keqiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3521718/
https://www.ncbi.nlm.nih.gov/pubmed/23271976
http://dx.doi.org/10.1371/journal.pgen.1003114
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author Luo, Ming
Yu, Chun-Wei
Chen, Fang-Fang
Zhao, Linmao
Tian, Gang
Liu, Xuncheng
Cui, Yuhai
Yang, Jun-Yi
Wu, Keqiang
author_facet Luo, Ming
Yu, Chun-Wei
Chen, Fang-Fang
Zhao, Linmao
Tian, Gang
Liu, Xuncheng
Cui, Yuhai
Yang, Jun-Yi
Wu, Keqiang
author_sort Luo, Ming
collection PubMed
description ASYMMETRIC LEAVES 1 (AS1) is a MYB-type transcription repressor that controls leaf development by regulating KNOX gene expression, but the underlying molecular mechanism is still unclear. In this study, we demonstrated that AS1 can interact with the histone deacetylase HDA6 in vitro and in vivo. The KNOX genes were up-regulated and hyperacetylated in the hda6 mutant, axe1-5, indicating that HDA6 may regulate KNOX expression through histone deacetylation. Compared with the single mutants, the as1-1/axe1-5 and as2-1/axe1-5 double mutants displayed more severe serrated leaf and short petiole phenotypes. In addition, the frequencies of leaf lobes and leaflet-like structures were also increased in as1-1/axe1-5 and as2-1/axe1-5 double mutants, suggesting that HDA6 acts together with AS1 and AS2 in regulating leaf development. Chromatin immunoprecipitation assays revealed that HDA6 and AS1 bound directly to KNAT1, KNAT2, and KNATM chromatin. Taken together, these data indicate that HDA6 is a part of the AS1 repressor complex to regulate the KNOX expression in leaf development.
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spelling pubmed-35217182012-12-27 Histone Deacetylase HDA6 Is Functionally Associated with AS1 in Repression of KNOX Genes in Arabidopsis Luo, Ming Yu, Chun-Wei Chen, Fang-Fang Zhao, Linmao Tian, Gang Liu, Xuncheng Cui, Yuhai Yang, Jun-Yi Wu, Keqiang PLoS Genet Research Article ASYMMETRIC LEAVES 1 (AS1) is a MYB-type transcription repressor that controls leaf development by regulating KNOX gene expression, but the underlying molecular mechanism is still unclear. In this study, we demonstrated that AS1 can interact with the histone deacetylase HDA6 in vitro and in vivo. The KNOX genes were up-regulated and hyperacetylated in the hda6 mutant, axe1-5, indicating that HDA6 may regulate KNOX expression through histone deacetylation. Compared with the single mutants, the as1-1/axe1-5 and as2-1/axe1-5 double mutants displayed more severe serrated leaf and short petiole phenotypes. In addition, the frequencies of leaf lobes and leaflet-like structures were also increased in as1-1/axe1-5 and as2-1/axe1-5 double mutants, suggesting that HDA6 acts together with AS1 and AS2 in regulating leaf development. Chromatin immunoprecipitation assays revealed that HDA6 and AS1 bound directly to KNAT1, KNAT2, and KNATM chromatin. Taken together, these data indicate that HDA6 is a part of the AS1 repressor complex to regulate the KNOX expression in leaf development. Public Library of Science 2012-12-13 /pmc/articles/PMC3521718/ /pubmed/23271976 http://dx.doi.org/10.1371/journal.pgen.1003114 Text en © 2012 Luo et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Luo, Ming
Yu, Chun-Wei
Chen, Fang-Fang
Zhao, Linmao
Tian, Gang
Liu, Xuncheng
Cui, Yuhai
Yang, Jun-Yi
Wu, Keqiang
Histone Deacetylase HDA6 Is Functionally Associated with AS1 in Repression of KNOX Genes in Arabidopsis
title Histone Deacetylase HDA6 Is Functionally Associated with AS1 in Repression of KNOX Genes in Arabidopsis
title_full Histone Deacetylase HDA6 Is Functionally Associated with AS1 in Repression of KNOX Genes in Arabidopsis
title_fullStr Histone Deacetylase HDA6 Is Functionally Associated with AS1 in Repression of KNOX Genes in Arabidopsis
title_full_unstemmed Histone Deacetylase HDA6 Is Functionally Associated with AS1 in Repression of KNOX Genes in Arabidopsis
title_short Histone Deacetylase HDA6 Is Functionally Associated with AS1 in Repression of KNOX Genes in Arabidopsis
title_sort histone deacetylase hda6 is functionally associated with as1 in repression of knox genes in arabidopsis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3521718/
https://www.ncbi.nlm.nih.gov/pubmed/23271976
http://dx.doi.org/10.1371/journal.pgen.1003114
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