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Flow cytometry-assisted rapid isolation of recombinant Plasmodium berghei parasites exemplified by functional analysis of aquaglyceroporin
The most critical bottleneck in the generation of recombinant Plasmodium berghei parasites is the mandatory in vivo cloning step following successful genetic manipulation. This study describes a new technique for rapid selection of recombinant P. berghei parasites. The method is based on flow cytome...
Autores principales: | Kenthirapalan, Sanketha, Waters, Andrew P., Matuschewski, Kai, Kooij, Taco W.A. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3521960/ https://www.ncbi.nlm.nih.gov/pubmed/23137753 http://dx.doi.org/10.1016/j.ijpara.2012.10.006 |
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