Ultra-Bright and -Stable Red and Near-Infrared Squaraine Fluorophores for In Vivo Two-Photon Imaging

Fluorescent dyes that are bright, stable, small, and biocompatible are needed for high-sensitivity two-photon imaging, but the combination of these traits has been elusive. We identified a class of squaraine derivatives with large two-photon action cross-sections (up to 10,000 GM) at near-infrared w...

Descripción completa

Detalles Bibliográficos
Autores principales: Podgorski, Kaspar, Terpetschnig, Ewald, Klochko, Oleksii P., Obukhova, Olena M., Haas, Kurt
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3522634/
https://www.ncbi.nlm.nih.gov/pubmed/23251670
http://dx.doi.org/10.1371/journal.pone.0051980
_version_ 1782253103610855424
author Podgorski, Kaspar
Terpetschnig, Ewald
Klochko, Oleksii P.
Obukhova, Olena M.
Haas, Kurt
author_facet Podgorski, Kaspar
Terpetschnig, Ewald
Klochko, Oleksii P.
Obukhova, Olena M.
Haas, Kurt
author_sort Podgorski, Kaspar
collection PubMed
description Fluorescent dyes that are bright, stable, small, and biocompatible are needed for high-sensitivity two-photon imaging, but the combination of these traits has been elusive. We identified a class of squaraine derivatives with large two-photon action cross-sections (up to 10,000 GM) at near-infrared wavelengths critical for in vivo imaging. We demonstrate the biocompatibility and stability of a red-emitting squaraine-rotaxane (SeTau-647) by imaging dye-filled neurons in vivo over 5 days, and utility for sensitive subcellular imaging by synthesizing a specific peptide-conjugate label for the synaptic protein PSD-95.
format Online
Article
Text
id pubmed-3522634
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-35226342012-12-18 Ultra-Bright and -Stable Red and Near-Infrared Squaraine Fluorophores for In Vivo Two-Photon Imaging Podgorski, Kaspar Terpetschnig, Ewald Klochko, Oleksii P. Obukhova, Olena M. Haas, Kurt PLoS One Research Article Fluorescent dyes that are bright, stable, small, and biocompatible are needed for high-sensitivity two-photon imaging, but the combination of these traits has been elusive. We identified a class of squaraine derivatives with large two-photon action cross-sections (up to 10,000 GM) at near-infrared wavelengths critical for in vivo imaging. We demonstrate the biocompatibility and stability of a red-emitting squaraine-rotaxane (SeTau-647) by imaging dye-filled neurons in vivo over 5 days, and utility for sensitive subcellular imaging by synthesizing a specific peptide-conjugate label for the synaptic protein PSD-95. Public Library of Science 2012-12-14 /pmc/articles/PMC3522634/ /pubmed/23251670 http://dx.doi.org/10.1371/journal.pone.0051980 Text en © 2012 Podgorski et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Podgorski, Kaspar
Terpetschnig, Ewald
Klochko, Oleksii P.
Obukhova, Olena M.
Haas, Kurt
Ultra-Bright and -Stable Red and Near-Infrared Squaraine Fluorophores for In Vivo Two-Photon Imaging
title Ultra-Bright and -Stable Red and Near-Infrared Squaraine Fluorophores for In Vivo Two-Photon Imaging
title_full Ultra-Bright and -Stable Red and Near-Infrared Squaraine Fluorophores for In Vivo Two-Photon Imaging
title_fullStr Ultra-Bright and -Stable Red and Near-Infrared Squaraine Fluorophores for In Vivo Two-Photon Imaging
title_full_unstemmed Ultra-Bright and -Stable Red and Near-Infrared Squaraine Fluorophores for In Vivo Two-Photon Imaging
title_short Ultra-Bright and -Stable Red and Near-Infrared Squaraine Fluorophores for In Vivo Two-Photon Imaging
title_sort ultra-bright and -stable red and near-infrared squaraine fluorophores for in vivo two-photon imaging
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3522634/
https://www.ncbi.nlm.nih.gov/pubmed/23251670
http://dx.doi.org/10.1371/journal.pone.0051980
work_keys_str_mv AT podgorskikaspar ultrabrightandstableredandnearinfraredsquarainefluorophoresforinvivotwophotonimaging
AT terpetschnigewald ultrabrightandstableredandnearinfraredsquarainefluorophoresforinvivotwophotonimaging
AT klochkooleksiip ultrabrightandstableredandnearinfraredsquarainefluorophoresforinvivotwophotonimaging
AT obukhovaolenam ultrabrightandstableredandnearinfraredsquarainefluorophoresforinvivotwophotonimaging
AT haaskurt ultrabrightandstableredandnearinfraredsquarainefluorophoresforinvivotwophotonimaging

Ejemplares similares