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Scanning Ion Conductance Microscopy for Studying Biological Samples

Scanning ion conductance microscopy (SICM) is a scanning probe technique that utilizes the increase in access resistance that occurs if an electrolyte filled glass micro-pipette is approached towards a poorly conducting surface. Since an increase in resistance can be monitored before the physical co...

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Detalles Bibliográficos
Autores principales: Happel, Patrick, Thatenhorst, Denis, Dietzel, Irmgard D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3522950/
https://www.ncbi.nlm.nih.gov/pubmed/23202197
http://dx.doi.org/10.3390/s121114983
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author Happel, Patrick
Thatenhorst, Denis
Dietzel, Irmgard D.
author_facet Happel, Patrick
Thatenhorst, Denis
Dietzel, Irmgard D.
author_sort Happel, Patrick
collection PubMed
description Scanning ion conductance microscopy (SICM) is a scanning probe technique that utilizes the increase in access resistance that occurs if an electrolyte filled glass micro-pipette is approached towards a poorly conducting surface. Since an increase in resistance can be monitored before the physical contact between scanning probe tip and sample, this technique is particularly useful to investigate the topography of delicate samples such as living cells. SICM has shown its potential in various applications such as high resolution and long-time imaging of living cells or the determination of local changes in cellular volume. Furthermore, SICM has been combined with various techniques such as fluorescence microscopy or patch clamping to reveal localized information about proteins or protein functions. This review details the various advantages and pitfalls of SICM and provides an overview of the recent developments and applications of SICM in biological imaging. Furthermore, we show that in principle, a combination of SICM and ion selective micro-electrodes enables one to monitor the local ion activity surrounding a living cell.
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spelling pubmed-35229502013-01-09 Scanning Ion Conductance Microscopy for Studying Biological Samples Happel, Patrick Thatenhorst, Denis Dietzel, Irmgard D. Sensors (Basel) Review Scanning ion conductance microscopy (SICM) is a scanning probe technique that utilizes the increase in access resistance that occurs if an electrolyte filled glass micro-pipette is approached towards a poorly conducting surface. Since an increase in resistance can be monitored before the physical contact between scanning probe tip and sample, this technique is particularly useful to investigate the topography of delicate samples such as living cells. SICM has shown its potential in various applications such as high resolution and long-time imaging of living cells or the determination of local changes in cellular volume. Furthermore, SICM has been combined with various techniques such as fluorescence microscopy or patch clamping to reveal localized information about proteins or protein functions. This review details the various advantages and pitfalls of SICM and provides an overview of the recent developments and applications of SICM in biological imaging. Furthermore, we show that in principle, a combination of SICM and ion selective micro-electrodes enables one to monitor the local ion activity surrounding a living cell. Molecular Diversity Preservation International (MDPI) 2012-11-06 /pmc/articles/PMC3522950/ /pubmed/23202197 http://dx.doi.org/10.3390/s121114983 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Review
Happel, Patrick
Thatenhorst, Denis
Dietzel, Irmgard D.
Scanning Ion Conductance Microscopy for Studying Biological Samples
title Scanning Ion Conductance Microscopy for Studying Biological Samples
title_full Scanning Ion Conductance Microscopy for Studying Biological Samples
title_fullStr Scanning Ion Conductance Microscopy for Studying Biological Samples
title_full_unstemmed Scanning Ion Conductance Microscopy for Studying Biological Samples
title_short Scanning Ion Conductance Microscopy for Studying Biological Samples
title_sort scanning ion conductance microscopy for studying biological samples
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3522950/
https://www.ncbi.nlm.nih.gov/pubmed/23202197
http://dx.doi.org/10.3390/s121114983
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