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Scanning Ion Conductance Microscopy for Studying Biological Samples
Scanning ion conductance microscopy (SICM) is a scanning probe technique that utilizes the increase in access resistance that occurs if an electrolyte filled glass micro-pipette is approached towards a poorly conducting surface. Since an increase in resistance can be monitored before the physical co...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3522950/ https://www.ncbi.nlm.nih.gov/pubmed/23202197 http://dx.doi.org/10.3390/s121114983 |
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author | Happel, Patrick Thatenhorst, Denis Dietzel, Irmgard D. |
author_facet | Happel, Patrick Thatenhorst, Denis Dietzel, Irmgard D. |
author_sort | Happel, Patrick |
collection | PubMed |
description | Scanning ion conductance microscopy (SICM) is a scanning probe technique that utilizes the increase in access resistance that occurs if an electrolyte filled glass micro-pipette is approached towards a poorly conducting surface. Since an increase in resistance can be monitored before the physical contact between scanning probe tip and sample, this technique is particularly useful to investigate the topography of delicate samples such as living cells. SICM has shown its potential in various applications such as high resolution and long-time imaging of living cells or the determination of local changes in cellular volume. Furthermore, SICM has been combined with various techniques such as fluorescence microscopy or patch clamping to reveal localized information about proteins or protein functions. This review details the various advantages and pitfalls of SICM and provides an overview of the recent developments and applications of SICM in biological imaging. Furthermore, we show that in principle, a combination of SICM and ion selective micro-electrodes enables one to monitor the local ion activity surrounding a living cell. |
format | Online Article Text |
id | pubmed-3522950 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-35229502013-01-09 Scanning Ion Conductance Microscopy for Studying Biological Samples Happel, Patrick Thatenhorst, Denis Dietzel, Irmgard D. Sensors (Basel) Review Scanning ion conductance microscopy (SICM) is a scanning probe technique that utilizes the increase in access resistance that occurs if an electrolyte filled glass micro-pipette is approached towards a poorly conducting surface. Since an increase in resistance can be monitored before the physical contact between scanning probe tip and sample, this technique is particularly useful to investigate the topography of delicate samples such as living cells. SICM has shown its potential in various applications such as high resolution and long-time imaging of living cells or the determination of local changes in cellular volume. Furthermore, SICM has been combined with various techniques such as fluorescence microscopy or patch clamping to reveal localized information about proteins or protein functions. This review details the various advantages and pitfalls of SICM and provides an overview of the recent developments and applications of SICM in biological imaging. Furthermore, we show that in principle, a combination of SICM and ion selective micro-electrodes enables one to monitor the local ion activity surrounding a living cell. Molecular Diversity Preservation International (MDPI) 2012-11-06 /pmc/articles/PMC3522950/ /pubmed/23202197 http://dx.doi.org/10.3390/s121114983 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Review Happel, Patrick Thatenhorst, Denis Dietzel, Irmgard D. Scanning Ion Conductance Microscopy for Studying Biological Samples |
title | Scanning Ion Conductance Microscopy for Studying Biological Samples |
title_full | Scanning Ion Conductance Microscopy for Studying Biological Samples |
title_fullStr | Scanning Ion Conductance Microscopy for Studying Biological Samples |
title_full_unstemmed | Scanning Ion Conductance Microscopy for Studying Biological Samples |
title_short | Scanning Ion Conductance Microscopy for Studying Biological Samples |
title_sort | scanning ion conductance microscopy for studying biological samples |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3522950/ https://www.ncbi.nlm.nih.gov/pubmed/23202197 http://dx.doi.org/10.3390/s121114983 |
work_keys_str_mv | AT happelpatrick scanningionconductancemicroscopyforstudyingbiologicalsamples AT thatenhorstdenis scanningionconductancemicroscopyforstudyingbiologicalsamples AT dietzelirmgardd scanningionconductancemicroscopyforstudyingbiologicalsamples |