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Anti-proliferative effect of Flos Albiziae flavonoids on the human gastric cancer SGC-7901 cell line

The flavonoids found in many foods may have a protective effect against human gastric cancer. However, little information is available concerning the effects of flavonoids on the SGC-7901 cell line. Therefore, we first evaluated the effects of purified Flos Albiziae flavonoids (FAFs) on the prolifer...

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Autores principales: YUAN, JIANMEI, LI, WANLI, TIAN, YUHUI, WANG, XIA
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3524100/
https://www.ncbi.nlm.nih.gov/pubmed/23251241
http://dx.doi.org/10.3892/etm.2012.771
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author YUAN, JIANMEI
LI, WANLI
TIAN, YUHUI
WANG, XIA
author_facet YUAN, JIANMEI
LI, WANLI
TIAN, YUHUI
WANG, XIA
author_sort YUAN, JIANMEI
collection PubMed
description The flavonoids found in many foods may have a protective effect against human gastric cancer. However, little information is available concerning the effects of flavonoids on the SGC-7901 cell line. Therefore, we first evaluated the effects of purified Flos Albiziae flavonoids (FAFs) on the proliferation of the SGC-7901 human gastric cancer cell line and investigated its possible anti-proliferative mechanisms. When SGC-7901 cells were treated with FAFs for various time periods (12–72 h) and at various doses (0–32 μg/ml), cell growth decreased significantly in a time- and dose-dependent manner. Morphological observations with fluorescence microscopy and transmission electron microscopy (TEM) yielded clear evidence of cell shrinkage, formation of cytoplasmic filaments, condensation of nuclear chromatin, and cell apoptosis in the presence of FAFs. Treatment with FAFs changed the expression levels of Bcl-2, P65, Bax and caspase. The anti-apoptotic protein expression of Bcl-2 and p65 decreased gradually with the increase in FAF concentration, compared with control cells (P<0.05). FAFs contributed to the increase in Bax and caspase expression. The expression of pro-apoptotic proteins Bax and caspase were upregulated by FAFs compared with control cells (P<0.01). These results demonstrated that FAFs effectively induced apoptosis in the SGC-7901 cell line. This indicates that FAFs are likely to possess anticancer activity.
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spelling pubmed-35241002012-12-18 Anti-proliferative effect of Flos Albiziae flavonoids on the human gastric cancer SGC-7901 cell line YUAN, JIANMEI LI, WANLI TIAN, YUHUI WANG, XIA Exp Ther Med Articles The flavonoids found in many foods may have a protective effect against human gastric cancer. However, little information is available concerning the effects of flavonoids on the SGC-7901 cell line. Therefore, we first evaluated the effects of purified Flos Albiziae flavonoids (FAFs) on the proliferation of the SGC-7901 human gastric cancer cell line and investigated its possible anti-proliferative mechanisms. When SGC-7901 cells were treated with FAFs for various time periods (12–72 h) and at various doses (0–32 μg/ml), cell growth decreased significantly in a time- and dose-dependent manner. Morphological observations with fluorescence microscopy and transmission electron microscopy (TEM) yielded clear evidence of cell shrinkage, formation of cytoplasmic filaments, condensation of nuclear chromatin, and cell apoptosis in the presence of FAFs. Treatment with FAFs changed the expression levels of Bcl-2, P65, Bax and caspase. The anti-apoptotic protein expression of Bcl-2 and p65 decreased gradually with the increase in FAF concentration, compared with control cells (P<0.05). FAFs contributed to the increase in Bax and caspase expression. The expression of pro-apoptotic proteins Bax and caspase were upregulated by FAFs compared with control cells (P<0.01). These results demonstrated that FAFs effectively induced apoptosis in the SGC-7901 cell line. This indicates that FAFs are likely to possess anticancer activity. D.A. Spandidos 2013-01 2012-10-26 /pmc/articles/PMC3524100/ /pubmed/23251241 http://dx.doi.org/10.3892/etm.2012.771 Text en Copyright © 2013, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
YUAN, JIANMEI
LI, WANLI
TIAN, YUHUI
WANG, XIA
Anti-proliferative effect of Flos Albiziae flavonoids on the human gastric cancer SGC-7901 cell line
title Anti-proliferative effect of Flos Albiziae flavonoids on the human gastric cancer SGC-7901 cell line
title_full Anti-proliferative effect of Flos Albiziae flavonoids on the human gastric cancer SGC-7901 cell line
title_fullStr Anti-proliferative effect of Flos Albiziae flavonoids on the human gastric cancer SGC-7901 cell line
title_full_unstemmed Anti-proliferative effect of Flos Albiziae flavonoids on the human gastric cancer SGC-7901 cell line
title_short Anti-proliferative effect of Flos Albiziae flavonoids on the human gastric cancer SGC-7901 cell line
title_sort anti-proliferative effect of flos albiziae flavonoids on the human gastric cancer sgc-7901 cell line
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3524100/
https://www.ncbi.nlm.nih.gov/pubmed/23251241
http://dx.doi.org/10.3892/etm.2012.771
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