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Oral administration of monogalactosyl diacylglycerol from spinach inhibits colon tumor growth in mice

Previously, we observed that purified monogalactosyl diacylglycerol (MGDG), a major glycoglycerolipid from spinach, selectively inhibits the activities of mammalian replicative DNA polymerases (α, δ and ε). However, the function of MGDG following ingestion is not well-known. In the present study, sp...

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Autores principales: MAEDA, NAOKI, KOKAI, YASUO, HADA, TAKAHIKO, YOSHIDA, HIROMI, MIZUSHINA, YOSHIYUKI
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3524182/
https://www.ncbi.nlm.nih.gov/pubmed/23251235
http://dx.doi.org/10.3892/etm.2012.792
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author MAEDA, NAOKI
KOKAI, YASUO
HADA, TAKAHIKO
YOSHIDA, HIROMI
MIZUSHINA, YOSHIYUKI
author_facet MAEDA, NAOKI
KOKAI, YASUO
HADA, TAKAHIKO
YOSHIDA, HIROMI
MIZUSHINA, YOSHIYUKI
author_sort MAEDA, NAOKI
collection PubMed
description Previously, we observed that purified monogalactosyl diacylglycerol (MGDG), a major glycoglycerolipid from spinach, selectively inhibits the activities of mammalian replicative DNA polymerases (α, δ and ε). However, the function of MGDG following ingestion is not well-known. In the present study, spinach MGDG suppressed the proliferation of Colon26 mouse colon cancer cells with an LD(50) of 24 μg/ml in vitro. γ-cyclodextrin (CD)-MGDG complex was prepared and administered orally following Colon26 mouse tumor adhesion for 26 days. It was observed that 20 mg/kg equivalent (eq.) of the CD-MGDG complex reduced tumor volume by ∼60% compared with that of the vehicle-treated controls. In immunohistochemical analysis, the CD-MGDG complex group showed a decreased number of proliferating cell nuclear antigen (PCNA)-positive cells and reduction of mitosis in the tumor cells compared with the control group. In addition, the CD-MGDG complex increased the number of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive apoptotic cells and inhibited CD31-positive tumor blood vessel growth significantly. These results suggest that MGDG has the potential for cancer prevention and health promotion.
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spelling pubmed-35241822012-12-18 Oral administration of monogalactosyl diacylglycerol from spinach inhibits colon tumor growth in mice MAEDA, NAOKI KOKAI, YASUO HADA, TAKAHIKO YOSHIDA, HIROMI MIZUSHINA, YOSHIYUKI Exp Ther Med Articles Previously, we observed that purified monogalactosyl diacylglycerol (MGDG), a major glycoglycerolipid from spinach, selectively inhibits the activities of mammalian replicative DNA polymerases (α, δ and ε). However, the function of MGDG following ingestion is not well-known. In the present study, spinach MGDG suppressed the proliferation of Colon26 mouse colon cancer cells with an LD(50) of 24 μg/ml in vitro. γ-cyclodextrin (CD)-MGDG complex was prepared and administered orally following Colon26 mouse tumor adhesion for 26 days. It was observed that 20 mg/kg equivalent (eq.) of the CD-MGDG complex reduced tumor volume by ∼60% compared with that of the vehicle-treated controls. In immunohistochemical analysis, the CD-MGDG complex group showed a decreased number of proliferating cell nuclear antigen (PCNA)-positive cells and reduction of mitosis in the tumor cells compared with the control group. In addition, the CD-MGDG complex increased the number of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive apoptotic cells and inhibited CD31-positive tumor blood vessel growth significantly. These results suggest that MGDG has the potential for cancer prevention and health promotion. D.A. Spandidos 2013-01 2012-11-01 /pmc/articles/PMC3524182/ /pubmed/23251235 http://dx.doi.org/10.3892/etm.2012.792 Text en Copyright © 2013, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
MAEDA, NAOKI
KOKAI, YASUO
HADA, TAKAHIKO
YOSHIDA, HIROMI
MIZUSHINA, YOSHIYUKI
Oral administration of monogalactosyl diacylglycerol from spinach inhibits colon tumor growth in mice
title Oral administration of monogalactosyl diacylglycerol from spinach inhibits colon tumor growth in mice
title_full Oral administration of monogalactosyl diacylglycerol from spinach inhibits colon tumor growth in mice
title_fullStr Oral administration of monogalactosyl diacylglycerol from spinach inhibits colon tumor growth in mice
title_full_unstemmed Oral administration of monogalactosyl diacylglycerol from spinach inhibits colon tumor growth in mice
title_short Oral administration of monogalactosyl diacylglycerol from spinach inhibits colon tumor growth in mice
title_sort oral administration of monogalactosyl diacylglycerol from spinach inhibits colon tumor growth in mice
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3524182/
https://www.ncbi.nlm.nih.gov/pubmed/23251235
http://dx.doi.org/10.3892/etm.2012.792
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