Cargando…

Analysis of the expression of KIR and HLA-Cw in a Northeast Han population

The aim of this study was to investigate the expression of the human leukocyte antigen (HLA)-Cw and killer cell immunoglobulin-like receptor (KIR) genes in a Jilin Han population and to provide a theoretical basis for further studies of their roles in disease. A total of 154 unpaid Jilin Han blood d...

Descripción completa

Detalles Bibliográficos
Autores principales: HAN, YU, ZHAO, LING, JIANG, ZHENYU, MA, NING
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3524289/
https://www.ncbi.nlm.nih.gov/pubmed/23251287
http://dx.doi.org/10.3892/etm.2012.763
_version_ 1782253306084589568
author HAN, YU
ZHAO, LING
JIANG, ZHENYU
MA, NING
author_facet HAN, YU
ZHAO, LING
JIANG, ZHENYU
MA, NING
author_sort HAN, YU
collection PubMed
description The aim of this study was to investigate the expression of the human leukocyte antigen (HLA)-Cw and killer cell immunoglobulin-like receptor (KIR) genes in a Jilin Han population and to provide a theoretical basis for further studies of their roles in disease. A total of 154 unpaid Jilin Han blood donors were selected and KIR and HLA-Cw genotyping was performed using PCR-SSP. Recognition of HLA-Cw and the corresponding activatory or inhibitory KIR receptor was distinguished according to the identification of HLA-Cw and KIR. In the present study, the expression frequency of HLA-C2(Lys80)+2DL1 was 27.27%, HLA-C1(Asn80)+2DL2/2DL3 was 68.83%, 2DS2+HLA-C1(Asn80) was 9.74% and 2DS1+HLA-C2(Lys80) was 9.74%. Of the individuals in the study, 72.08% expressed only KIR2DL1 without HLA-Cw, 21.43% expressed only KIR2DS1 without HLA-Cw(Lys)-KIR2DL1 and 2.60% expressed only KIR2DS2 without HLA-Cw(Asn)-KIR2DL2/L3. In conclusion, the expression of inhibitory HLA-Cw-KIR is higher than the expression of activating HLA-Cw-KIR and approximately 20% of the individuals separately expressed the activated HLA-Cw-KIR in the Jilin Han population in the present study.
format Online
Article
Text
id pubmed-3524289
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher D.A. Spandidos
record_format MEDLINE/PubMed
spelling pubmed-35242892012-12-18 Analysis of the expression of KIR and HLA-Cw in a Northeast Han population HAN, YU ZHAO, LING JIANG, ZHENYU MA, NING Exp Ther Med Articles The aim of this study was to investigate the expression of the human leukocyte antigen (HLA)-Cw and killer cell immunoglobulin-like receptor (KIR) genes in a Jilin Han population and to provide a theoretical basis for further studies of their roles in disease. A total of 154 unpaid Jilin Han blood donors were selected and KIR and HLA-Cw genotyping was performed using PCR-SSP. Recognition of HLA-Cw and the corresponding activatory or inhibitory KIR receptor was distinguished according to the identification of HLA-Cw and KIR. In the present study, the expression frequency of HLA-C2(Lys80)+2DL1 was 27.27%, HLA-C1(Asn80)+2DL2/2DL3 was 68.83%, 2DS2+HLA-C1(Asn80) was 9.74% and 2DS1+HLA-C2(Lys80) was 9.74%. Of the individuals in the study, 72.08% expressed only KIR2DL1 without HLA-Cw, 21.43% expressed only KIR2DS1 without HLA-Cw(Lys)-KIR2DL1 and 2.60% expressed only KIR2DS2 without HLA-Cw(Asn)-KIR2DL2/L3. In conclusion, the expression of inhibitory HLA-Cw-KIR is higher than the expression of activating HLA-Cw-KIR and approximately 20% of the individuals separately expressed the activated HLA-Cw-KIR in the Jilin Han population in the present study. D.A. Spandidos 2013-01 2012-10-25 /pmc/articles/PMC3524289/ /pubmed/23251287 http://dx.doi.org/10.3892/etm.2012.763 Text en Copyright © 2013, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
HAN, YU
ZHAO, LING
JIANG, ZHENYU
MA, NING
Analysis of the expression of KIR and HLA-Cw in a Northeast Han population
title Analysis of the expression of KIR and HLA-Cw in a Northeast Han population
title_full Analysis of the expression of KIR and HLA-Cw in a Northeast Han population
title_fullStr Analysis of the expression of KIR and HLA-Cw in a Northeast Han population
title_full_unstemmed Analysis of the expression of KIR and HLA-Cw in a Northeast Han population
title_short Analysis of the expression of KIR and HLA-Cw in a Northeast Han population
title_sort analysis of the expression of kir and hla-cw in a northeast han population
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3524289/
https://www.ncbi.nlm.nih.gov/pubmed/23251287
http://dx.doi.org/10.3892/etm.2012.763
work_keys_str_mv AT hanyu analysisoftheexpressionofkirandhlacwinanortheasthanpopulation
AT zhaoling analysisoftheexpressionofkirandhlacwinanortheasthanpopulation
AT jiangzhenyu analysisoftheexpressionofkirandhlacwinanortheasthanpopulation
AT maning analysisoftheexpressionofkirandhlacwinanortheasthanpopulation