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Phenotypic and genotypic study of macrolide, lincosamide and streptogramin B (MLS(B)) resistance in clinical isolates of Staphylococcus aureus in Tehran, Iran
BACKGROUND: Resistance to antimicrobial agents among Staphylococcus aureus is an increasing problem. Two common genes responsible for resistance to macrolide, lincosamide and streptogramin B (MLS(B)) antibiotics are the ermA and ermC genes. Three resistance phenotypes have been detected to these ant...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Scientific Literature, Inc.
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3524716/ https://www.ncbi.nlm.nih.gov/pubmed/21278685 http://dx.doi.org/10.12659/MSM.881386 |
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author | Saderi, Horieh Emadi, Behzad Owlia, Parviz |
author_facet | Saderi, Horieh Emadi, Behzad Owlia, Parviz |
author_sort | Saderi, Horieh |
collection | PubMed |
description | BACKGROUND: Resistance to antimicrobial agents among Staphylococcus aureus is an increasing problem. Two common genes responsible for resistance to macrolide, lincosamide and streptogramin B (MLS(B)) antibiotics are the ermA and ermC genes. Three resistance phenotypes have been detected to these antibiotics: strains containing cMLS(B) (constitutive MLS(B)) and iMLS(B) (inducible MLS(B)), which are resistant to macrolide, lincosamide and streptogramin B antibiotics, and MS, which is only resistant to macrolide and streptogramin B antibiotics. The aim of this study was to determine the prevalence of MLS(B) phenotypes and genotypes in erythromycin-resistant strains of S. aureus isolated from patients in 4 university hospitals in Tehran, Iran. MATERIAL/METHODS: S. aureus strains were isolated from various clinical specimens and identified by routine phenotypic methods and PCR for nuc gene. Erythromycin resistance was determined by disk diffusion testing. Prevalence of MLS(B) phenotypes was determined by use of the D-test. ermA and ermC genes were detected by PCR. RESULTS: Altogether, 126 erythromycin-resistant strains of S. aureus were detected. Prevalence of cMLS(B), iMLS(B) and MS resistance phenotypes were 92.8%, 6.4%, and 0.8%, respectively; 60.3% of strains had ermA gene and 54.8% ermC gene; 61 strains (48.4%) contained 2 studied erm genes and 42 strains (33.3%) did not have any studied erm genes. CONCLUSIONS: Due to the high prevalence of clindamycin resistance among S. aureus isolated from patients in Iran, we recommend clindamycin therapy only after proper antimicrobial susceptibility testing. |
format | Online Article Text |
id | pubmed-3524716 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | International Scientific Literature, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-35247162013-04-24 Phenotypic and genotypic study of macrolide, lincosamide and streptogramin B (MLS(B)) resistance in clinical isolates of Staphylococcus aureus in Tehran, Iran Saderi, Horieh Emadi, Behzad Owlia, Parviz Med Sci Monit Basic Research BACKGROUND: Resistance to antimicrobial agents among Staphylococcus aureus is an increasing problem. Two common genes responsible for resistance to macrolide, lincosamide and streptogramin B (MLS(B)) antibiotics are the ermA and ermC genes. Three resistance phenotypes have been detected to these antibiotics: strains containing cMLS(B) (constitutive MLS(B)) and iMLS(B) (inducible MLS(B)), which are resistant to macrolide, lincosamide and streptogramin B antibiotics, and MS, which is only resistant to macrolide and streptogramin B antibiotics. The aim of this study was to determine the prevalence of MLS(B) phenotypes and genotypes in erythromycin-resistant strains of S. aureus isolated from patients in 4 university hospitals in Tehran, Iran. MATERIAL/METHODS: S. aureus strains were isolated from various clinical specimens and identified by routine phenotypic methods and PCR for nuc gene. Erythromycin resistance was determined by disk diffusion testing. Prevalence of MLS(B) phenotypes was determined by use of the D-test. ermA and ermC genes were detected by PCR. RESULTS: Altogether, 126 erythromycin-resistant strains of S. aureus were detected. Prevalence of cMLS(B), iMLS(B) and MS resistance phenotypes were 92.8%, 6.4%, and 0.8%, respectively; 60.3% of strains had ermA gene and 54.8% ermC gene; 61 strains (48.4%) contained 2 studied erm genes and 42 strains (33.3%) did not have any studied erm genes. CONCLUSIONS: Due to the high prevalence of clindamycin resistance among S. aureus isolated from patients in Iran, we recommend clindamycin therapy only after proper antimicrobial susceptibility testing. International Scientific Literature, Inc. 2011-02-01 /pmc/articles/PMC3524716/ /pubmed/21278685 http://dx.doi.org/10.12659/MSM.881386 Text en © Med Sci Monit, 2011 This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. |
spellingShingle | Basic Research Saderi, Horieh Emadi, Behzad Owlia, Parviz Phenotypic and genotypic study of macrolide, lincosamide and streptogramin B (MLS(B)) resistance in clinical isolates of Staphylococcus aureus in Tehran, Iran |
title | Phenotypic and genotypic study of macrolide, lincosamide and streptogramin B (MLS(B)) resistance in clinical isolates of Staphylococcus aureus in Tehran, Iran |
title_full | Phenotypic and genotypic study of macrolide, lincosamide and streptogramin B (MLS(B)) resistance in clinical isolates of Staphylococcus aureus in Tehran, Iran |
title_fullStr | Phenotypic and genotypic study of macrolide, lincosamide and streptogramin B (MLS(B)) resistance in clinical isolates of Staphylococcus aureus in Tehran, Iran |
title_full_unstemmed | Phenotypic and genotypic study of macrolide, lincosamide and streptogramin B (MLS(B)) resistance in clinical isolates of Staphylococcus aureus in Tehran, Iran |
title_short | Phenotypic and genotypic study of macrolide, lincosamide and streptogramin B (MLS(B)) resistance in clinical isolates of Staphylococcus aureus in Tehran, Iran |
title_sort | phenotypic and genotypic study of macrolide, lincosamide and streptogramin b (mls(b)) resistance in clinical isolates of staphylococcus aureus in tehran, iran |
topic | Basic Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3524716/ https://www.ncbi.nlm.nih.gov/pubmed/21278685 http://dx.doi.org/10.12659/MSM.881386 |
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