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Morphologic diversity of cutaneous sensory afferents revealed by genetically directed sparse labeling
The diversity of cutaneous sensory afferents has been studied by many investigators using behavioral, physiologic, molecular, and genetic approaches. Largely missing, thus far, is an analysis of the complete morphologies of individual afferent arbors. Here we present a survey of cutaneous sensory ar...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3524796/ https://www.ncbi.nlm.nih.gov/pubmed/23256042 http://dx.doi.org/10.7554/eLife.00181 |
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author | Wu, Hao Williams, John Nathans, Jeremy |
author_facet | Wu, Hao Williams, John Nathans, Jeremy |
author_sort | Wu, Hao |
collection | PubMed |
description | The diversity of cutaneous sensory afferents has been studied by many investigators using behavioral, physiologic, molecular, and genetic approaches. Largely missing, thus far, is an analysis of the complete morphologies of individual afferent arbors. Here we present a survey of cutaneous sensory arbor morphologies in hairy skin of the mouse using genetically-directed sparse labeling with a sensory neuron-specific alkaline phosphatase reporter. Quantitative analyses of 719 arbors, among which 77 were fully reconstructed, reveal 10 morphologically distinct types. Among the two types with the largest arbors, one contacts ∼200 hair follicles with circumferential endings and a second is characterized by a densely ramifying arbor with one to several thousand branches and a total axon length between one-half and one meter. These observations constrain models of receptive field size and structure among cutaneous sensory neurons, and they raise intriguing questions regarding the cellular and developmental mechanisms responsible for this morphological diversity. DOI: http://dx.doi.org/10.7554/eLife.00181.001 |
format | Online Article Text |
id | pubmed-3524796 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-35247962012-12-19 Morphologic diversity of cutaneous sensory afferents revealed by genetically directed sparse labeling Wu, Hao Williams, John Nathans, Jeremy eLife Developmental Biology and Stem Cells The diversity of cutaneous sensory afferents has been studied by many investigators using behavioral, physiologic, molecular, and genetic approaches. Largely missing, thus far, is an analysis of the complete morphologies of individual afferent arbors. Here we present a survey of cutaneous sensory arbor morphologies in hairy skin of the mouse using genetically-directed sparse labeling with a sensory neuron-specific alkaline phosphatase reporter. Quantitative analyses of 719 arbors, among which 77 were fully reconstructed, reveal 10 morphologically distinct types. Among the two types with the largest arbors, one contacts ∼200 hair follicles with circumferential endings and a second is characterized by a densely ramifying arbor with one to several thousand branches and a total axon length between one-half and one meter. These observations constrain models of receptive field size and structure among cutaneous sensory neurons, and they raise intriguing questions regarding the cellular and developmental mechanisms responsible for this morphological diversity. DOI: http://dx.doi.org/10.7554/eLife.00181.001 eLife Sciences Publications, Ltd 2012-12-18 /pmc/articles/PMC3524796/ /pubmed/23256042 http://dx.doi.org/10.7554/eLife.00181 Text en Copyright © 2012, Wu et al http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Developmental Biology and Stem Cells Wu, Hao Williams, John Nathans, Jeremy Morphologic diversity of cutaneous sensory afferents revealed by genetically directed sparse labeling |
title | Morphologic diversity of cutaneous sensory afferents revealed by genetically directed sparse labeling |
title_full | Morphologic diversity of cutaneous sensory afferents revealed by genetically directed sparse labeling |
title_fullStr | Morphologic diversity of cutaneous sensory afferents revealed by genetically directed sparse labeling |
title_full_unstemmed | Morphologic diversity of cutaneous sensory afferents revealed by genetically directed sparse labeling |
title_short | Morphologic diversity of cutaneous sensory afferents revealed by genetically directed sparse labeling |
title_sort | morphologic diversity of cutaneous sensory afferents revealed by genetically directed sparse labeling |
topic | Developmental Biology and Stem Cells |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3524796/ https://www.ncbi.nlm.nih.gov/pubmed/23256042 http://dx.doi.org/10.7554/eLife.00181 |
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