Cargando…

Assessment of Anticholinesterase Activity of Gelidiella acerosa: Implications for Its Therapeutic Potential against Alzheimer's Disease

The effect of various solvent extracts of Gelidiella acerosa on acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) activities was investigated. AChE and BuChE inhibitory activities were analyzed by spectrophotometric method. Phytochemical screening of the compounds present in the solvent...

Descripción completa

Detalles Bibliográficos
Autores principales: Syad, Arif Nisha, Shunmugiah, Karutha Pandian, Kasi, Pandima Devi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3525188/
https://www.ncbi.nlm.nih.gov/pubmed/23304203
http://dx.doi.org/10.1155/2012/497242
_version_ 1782253405497982976
author Syad, Arif Nisha
Shunmugiah, Karutha Pandian
Kasi, Pandima Devi
author_facet Syad, Arif Nisha
Shunmugiah, Karutha Pandian
Kasi, Pandima Devi
author_sort Syad, Arif Nisha
collection PubMed
description The effect of various solvent extracts of Gelidiella acerosa on acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) activities was investigated. AChE and BuChE inhibitory activities were analyzed by spectrophotometric method. Phytochemical screening of the compounds present in the solvent extracts was done qualitatively. Characterization of the compounds present in the benzene extract of G. acerosa was done by GC-MS analysis. The results showed that, at 487.80 μg/mL, benzene extract showed significant (P < 0.05) inhibitory activity against both AChE and BuChE with the percentage of inhibition 54.18 ± 5.65 % (IC(50) = 434.61 ± 26.53 μg/mL) and 78.43 ± 0% (IC(50) = 163.01 ± 85.35 μg/mL), respectively. The mode of inhibition exhibited by benzene extract against the AChE and BuChE was found to be competitive and uncompetitive type of inhibition, respectively. Preliminary phytochemical analysis coupled with GC-MS illustrates that the benzene extract possesses high amount of terpenoids, which could be the reason for potential cholinesterase inhibitory activity.
format Online
Article
Text
id pubmed-3525188
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-35251882013-01-09 Assessment of Anticholinesterase Activity of Gelidiella acerosa: Implications for Its Therapeutic Potential against Alzheimer's Disease Syad, Arif Nisha Shunmugiah, Karutha Pandian Kasi, Pandima Devi Evid Based Complement Alternat Med Research Article The effect of various solvent extracts of Gelidiella acerosa on acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) activities was investigated. AChE and BuChE inhibitory activities were analyzed by spectrophotometric method. Phytochemical screening of the compounds present in the solvent extracts was done qualitatively. Characterization of the compounds present in the benzene extract of G. acerosa was done by GC-MS analysis. The results showed that, at 487.80 μg/mL, benzene extract showed significant (P < 0.05) inhibitory activity against both AChE and BuChE with the percentage of inhibition 54.18 ± 5.65 % (IC(50) = 434.61 ± 26.53 μg/mL) and 78.43 ± 0% (IC(50) = 163.01 ± 85.35 μg/mL), respectively. The mode of inhibition exhibited by benzene extract against the AChE and BuChE was found to be competitive and uncompetitive type of inhibition, respectively. Preliminary phytochemical analysis coupled with GC-MS illustrates that the benzene extract possesses high amount of terpenoids, which could be the reason for potential cholinesterase inhibitory activity. Hindawi Publishing Corporation 2012 2012-12-06 /pmc/articles/PMC3525188/ /pubmed/23304203 http://dx.doi.org/10.1155/2012/497242 Text en Copyright © 2012 Arif Nisha Syad et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Syad, Arif Nisha
Shunmugiah, Karutha Pandian
Kasi, Pandima Devi
Assessment of Anticholinesterase Activity of Gelidiella acerosa: Implications for Its Therapeutic Potential against Alzheimer's Disease
title Assessment of Anticholinesterase Activity of Gelidiella acerosa: Implications for Its Therapeutic Potential against Alzheimer's Disease
title_full Assessment of Anticholinesterase Activity of Gelidiella acerosa: Implications for Its Therapeutic Potential against Alzheimer's Disease
title_fullStr Assessment of Anticholinesterase Activity of Gelidiella acerosa: Implications for Its Therapeutic Potential against Alzheimer's Disease
title_full_unstemmed Assessment of Anticholinesterase Activity of Gelidiella acerosa: Implications for Its Therapeutic Potential against Alzheimer's Disease
title_short Assessment of Anticholinesterase Activity of Gelidiella acerosa: Implications for Its Therapeutic Potential against Alzheimer's Disease
title_sort assessment of anticholinesterase activity of gelidiella acerosa: implications for its therapeutic potential against alzheimer's disease
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3525188/
https://www.ncbi.nlm.nih.gov/pubmed/23304203
http://dx.doi.org/10.1155/2012/497242
work_keys_str_mv AT syadarifnisha assessmentofanticholinesteraseactivityofgelidiellaacerosaimplicationsforitstherapeuticpotentialagainstalzheimersdisease
AT shunmugiahkaruthapandian assessmentofanticholinesteraseactivityofgelidiellaacerosaimplicationsforitstherapeuticpotentialagainstalzheimersdisease
AT kasipandimadevi assessmentofanticholinesteraseactivityofgelidiellaacerosaimplicationsforitstherapeuticpotentialagainstalzheimersdisease