Cargando…
Assessment of Anticholinesterase Activity of Gelidiella acerosa: Implications for Its Therapeutic Potential against Alzheimer's Disease
The effect of various solvent extracts of Gelidiella acerosa on acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) activities was investigated. AChE and BuChE inhibitory activities were analyzed by spectrophotometric method. Phytochemical screening of the compounds present in the solvent...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3525188/ https://www.ncbi.nlm.nih.gov/pubmed/23304203 http://dx.doi.org/10.1155/2012/497242 |
_version_ | 1782253405497982976 |
---|---|
author | Syad, Arif Nisha Shunmugiah, Karutha Pandian Kasi, Pandima Devi |
author_facet | Syad, Arif Nisha Shunmugiah, Karutha Pandian Kasi, Pandima Devi |
author_sort | Syad, Arif Nisha |
collection | PubMed |
description | The effect of various solvent extracts of Gelidiella acerosa on acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) activities was investigated. AChE and BuChE inhibitory activities were analyzed by spectrophotometric method. Phytochemical screening of the compounds present in the solvent extracts was done qualitatively. Characterization of the compounds present in the benzene extract of G. acerosa was done by GC-MS analysis. The results showed that, at 487.80 μg/mL, benzene extract showed significant (P < 0.05) inhibitory activity against both AChE and BuChE with the percentage of inhibition 54.18 ± 5.65 % (IC(50) = 434.61 ± 26.53 μg/mL) and 78.43 ± 0% (IC(50) = 163.01 ± 85.35 μg/mL), respectively. The mode of inhibition exhibited by benzene extract against the AChE and BuChE was found to be competitive and uncompetitive type of inhibition, respectively. Preliminary phytochemical analysis coupled with GC-MS illustrates that the benzene extract possesses high amount of terpenoids, which could be the reason for potential cholinesterase inhibitory activity. |
format | Online Article Text |
id | pubmed-3525188 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-35251882013-01-09 Assessment of Anticholinesterase Activity of Gelidiella acerosa: Implications for Its Therapeutic Potential against Alzheimer's Disease Syad, Arif Nisha Shunmugiah, Karutha Pandian Kasi, Pandima Devi Evid Based Complement Alternat Med Research Article The effect of various solvent extracts of Gelidiella acerosa on acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) activities was investigated. AChE and BuChE inhibitory activities were analyzed by spectrophotometric method. Phytochemical screening of the compounds present in the solvent extracts was done qualitatively. Characterization of the compounds present in the benzene extract of G. acerosa was done by GC-MS analysis. The results showed that, at 487.80 μg/mL, benzene extract showed significant (P < 0.05) inhibitory activity against both AChE and BuChE with the percentage of inhibition 54.18 ± 5.65 % (IC(50) = 434.61 ± 26.53 μg/mL) and 78.43 ± 0% (IC(50) = 163.01 ± 85.35 μg/mL), respectively. The mode of inhibition exhibited by benzene extract against the AChE and BuChE was found to be competitive and uncompetitive type of inhibition, respectively. Preliminary phytochemical analysis coupled with GC-MS illustrates that the benzene extract possesses high amount of terpenoids, which could be the reason for potential cholinesterase inhibitory activity. Hindawi Publishing Corporation 2012 2012-12-06 /pmc/articles/PMC3525188/ /pubmed/23304203 http://dx.doi.org/10.1155/2012/497242 Text en Copyright © 2012 Arif Nisha Syad et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Syad, Arif Nisha Shunmugiah, Karutha Pandian Kasi, Pandima Devi Assessment of Anticholinesterase Activity of Gelidiella acerosa: Implications for Its Therapeutic Potential against Alzheimer's Disease |
title | Assessment of Anticholinesterase Activity of Gelidiella acerosa: Implications for Its Therapeutic Potential against Alzheimer's Disease |
title_full | Assessment of Anticholinesterase Activity of Gelidiella acerosa: Implications for Its Therapeutic Potential against Alzheimer's Disease |
title_fullStr | Assessment of Anticholinesterase Activity of Gelidiella acerosa: Implications for Its Therapeutic Potential against Alzheimer's Disease |
title_full_unstemmed | Assessment of Anticholinesterase Activity of Gelidiella acerosa: Implications for Its Therapeutic Potential against Alzheimer's Disease |
title_short | Assessment of Anticholinesterase Activity of Gelidiella acerosa: Implications for Its Therapeutic Potential against Alzheimer's Disease |
title_sort | assessment of anticholinesterase activity of gelidiella acerosa: implications for its therapeutic potential against alzheimer's disease |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3525188/ https://www.ncbi.nlm.nih.gov/pubmed/23304203 http://dx.doi.org/10.1155/2012/497242 |
work_keys_str_mv | AT syadarifnisha assessmentofanticholinesteraseactivityofgelidiellaacerosaimplicationsforitstherapeuticpotentialagainstalzheimersdisease AT shunmugiahkaruthapandian assessmentofanticholinesteraseactivityofgelidiellaacerosaimplicationsforitstherapeuticpotentialagainstalzheimersdisease AT kasipandimadevi assessmentofanticholinesteraseactivityofgelidiellaacerosaimplicationsforitstherapeuticpotentialagainstalzheimersdisease |