Cloning of the Quail PIWI Gene and Characterization of PIWI Binding to Small RNAs

The PIWI protein regulates gene expression at the epigenetic and post-transcriptional level with a variety of endogenous small non-coding RNAs. In poultry, the biological function of the PIWI protein and PIWI binding to small RNAs had not been determined. The present study cloned and analyzed the se...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Rong, Chang, Guobin, Zhang, Ying, Dai, Aiqin, Ma, Teng, Li, Jianchao, Zhai, Fei, Hua, Dengke, Xia, Mingxiu, Chen, Guohong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3526641/
https://www.ncbi.nlm.nih.gov/pubmed/23284755
http://dx.doi.org/10.1371/journal.pone.0051724
_version_ 1782253603616980992
author Chen, Rong
Chang, Guobin
Zhang, Ying
Dai, Aiqin
Ma, Teng
Li, Jianchao
Zhai, Fei
Hua, Dengke
Xia, Mingxiu
Chen, Guohong
author_facet Chen, Rong
Chang, Guobin
Zhang, Ying
Dai, Aiqin
Ma, Teng
Li, Jianchao
Zhai, Fei
Hua, Dengke
Xia, Mingxiu
Chen, Guohong
author_sort Chen, Rong
collection PubMed
description The PIWI protein regulates gene expression at the epigenetic and post-transcriptional level with a variety of endogenous small non-coding RNAs. In poultry, the biological function of the PIWI protein and PIWI binding to small RNAs had not been determined. The present study cloned and analyzed the sequences of the PIWIL1 protein. We also characterized PIWIL1 binding to small RNAs from adult quail testis, where the PIWIL1 protein is specifically expressed. Small RNAs showed a strong peak at 24–27 nt in the testicular RNA library, mapped primarily to repeat sequences and were similar to rasiRNAs. MicroRNAs (miRNAs) were abundant in the ovarian RNA library at a peak of 22 nt.
format Online
Article
Text
id pubmed-3526641
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-35266412013-01-02 Cloning of the Quail PIWI Gene and Characterization of PIWI Binding to Small RNAs Chen, Rong Chang, Guobin Zhang, Ying Dai, Aiqin Ma, Teng Li, Jianchao Zhai, Fei Hua, Dengke Xia, Mingxiu Chen, Guohong PLoS One Research Article The PIWI protein regulates gene expression at the epigenetic and post-transcriptional level with a variety of endogenous small non-coding RNAs. In poultry, the biological function of the PIWI protein and PIWI binding to small RNAs had not been determined. The present study cloned and analyzed the sequences of the PIWIL1 protein. We also characterized PIWIL1 binding to small RNAs from adult quail testis, where the PIWIL1 protein is specifically expressed. Small RNAs showed a strong peak at 24–27 nt in the testicular RNA library, mapped primarily to repeat sequences and were similar to rasiRNAs. MicroRNAs (miRNAs) were abundant in the ovarian RNA library at a peak of 22 nt. Public Library of Science 2012-12-19 /pmc/articles/PMC3526641/ /pubmed/23284755 http://dx.doi.org/10.1371/journal.pone.0051724 Text en © 2012 Chen et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Chen, Rong
Chang, Guobin
Zhang, Ying
Dai, Aiqin
Ma, Teng
Li, Jianchao
Zhai, Fei
Hua, Dengke
Xia, Mingxiu
Chen, Guohong
Cloning of the Quail PIWI Gene and Characterization of PIWI Binding to Small RNAs
title Cloning of the Quail PIWI Gene and Characterization of PIWI Binding to Small RNAs
title_full Cloning of the Quail PIWI Gene and Characterization of PIWI Binding to Small RNAs
title_fullStr Cloning of the Quail PIWI Gene and Characterization of PIWI Binding to Small RNAs
title_full_unstemmed Cloning of the Quail PIWI Gene and Characterization of PIWI Binding to Small RNAs
title_short Cloning of the Quail PIWI Gene and Characterization of PIWI Binding to Small RNAs
title_sort cloning of the quail piwi gene and characterization of piwi binding to small rnas
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3526641/
https://www.ncbi.nlm.nih.gov/pubmed/23284755
http://dx.doi.org/10.1371/journal.pone.0051724
work_keys_str_mv AT chenrong cloningofthequailpiwigeneandcharacterizationofpiwibindingtosmallrnas
AT changguobin cloningofthequailpiwigeneandcharacterizationofpiwibindingtosmallrnas
AT zhangying cloningofthequailpiwigeneandcharacterizationofpiwibindingtosmallrnas
AT daiaiqin cloningofthequailpiwigeneandcharacterizationofpiwibindingtosmallrnas
AT mateng cloningofthequailpiwigeneandcharacterizationofpiwibindingtosmallrnas
AT lijianchao cloningofthequailpiwigeneandcharacterizationofpiwibindingtosmallrnas
AT zhaifei cloningofthequailpiwigeneandcharacterizationofpiwibindingtosmallrnas
AT huadengke cloningofthequailpiwigeneandcharacterizationofpiwibindingtosmallrnas
AT xiamingxiu cloningofthequailpiwigeneandcharacterizationofpiwibindingtosmallrnas
AT chenguohong cloningofthequailpiwigeneandcharacterizationofpiwibindingtosmallrnas

Ejemplares similares