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Population and sex differences in Drosophila melanogaster brain gene expression
BACKGROUND: Changes in gene regulation are thought to be crucial for the adaptation of organisms to their environment. Transcriptome analyses can be used to identify candidate genes for ecological adaptation, but can be complicated by variation in gene expression between tissues, sexes, or individua...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3527002/ https://www.ncbi.nlm.nih.gov/pubmed/23170910 http://dx.doi.org/10.1186/1471-2164-13-654 |
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author | Catalán, Ana Hutter, Stephan Parsch, John |
author_facet | Catalán, Ana Hutter, Stephan Parsch, John |
author_sort | Catalán, Ana |
collection | PubMed |
description | BACKGROUND: Changes in gene regulation are thought to be crucial for the adaptation of organisms to their environment. Transcriptome analyses can be used to identify candidate genes for ecological adaptation, but can be complicated by variation in gene expression between tissues, sexes, or individuals. Here we use high-throughput RNA sequencing of a single Drosophila melanogaster tissue to detect brain-specific differences in gene expression between the sexes and between two populations, one from the ancestral species range in sub-Saharan Africa and one from the recently colonized species range in Europe. RESULTS: Relatively few genes (<100) displayed sexually dimorphic expression in the brain, but there was an enrichment of sex-biased genes, especially male-biased genes, on the X chromosome. Over 340 genes differed in brain expression between flies from the African and European populations, with the inter-population divergence being highly correlated between males and females. The differentially expressed genes included those involved in stress response, olfaction, and detoxification. Expression differences were associated with transposable element insertions at two genes implicated in insecticide resistance (Cyp6g1 and CHKov1). CONCLUSIONS: Analysis of the brain transcriptome revealed many genes differing in expression between populations that were not detected in previous studies using whole flies. There was little evidence for sex-specific regulatory adaptation in the brain, as most expression differences between populations were observed in both males and females. The enrichment of genes with sexually dimorphic expression on the X chromosome is consistent with dosage compensation mechanisms affecting sex-biased expression in somatic tissues. |
format | Online Article Text |
id | pubmed-3527002 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-35270022012-12-21 Population and sex differences in Drosophila melanogaster brain gene expression Catalán, Ana Hutter, Stephan Parsch, John BMC Genomics Research Article BACKGROUND: Changes in gene regulation are thought to be crucial for the adaptation of organisms to their environment. Transcriptome analyses can be used to identify candidate genes for ecological adaptation, but can be complicated by variation in gene expression between tissues, sexes, or individuals. Here we use high-throughput RNA sequencing of a single Drosophila melanogaster tissue to detect brain-specific differences in gene expression between the sexes and between two populations, one from the ancestral species range in sub-Saharan Africa and one from the recently colonized species range in Europe. RESULTS: Relatively few genes (<100) displayed sexually dimorphic expression in the brain, but there was an enrichment of sex-biased genes, especially male-biased genes, on the X chromosome. Over 340 genes differed in brain expression between flies from the African and European populations, with the inter-population divergence being highly correlated between males and females. The differentially expressed genes included those involved in stress response, olfaction, and detoxification. Expression differences were associated with transposable element insertions at two genes implicated in insecticide resistance (Cyp6g1 and CHKov1). CONCLUSIONS: Analysis of the brain transcriptome revealed many genes differing in expression between populations that were not detected in previous studies using whole flies. There was little evidence for sex-specific regulatory adaptation in the brain, as most expression differences between populations were observed in both males and females. The enrichment of genes with sexually dimorphic expression on the X chromosome is consistent with dosage compensation mechanisms affecting sex-biased expression in somatic tissues. BioMed Central 2012-11-21 /pmc/articles/PMC3527002/ /pubmed/23170910 http://dx.doi.org/10.1186/1471-2164-13-654 Text en Copyright ©2012 Catalán et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Catalán, Ana Hutter, Stephan Parsch, John Population and sex differences in Drosophila melanogaster brain gene expression |
title | Population and sex differences in Drosophila melanogaster brain gene expression |
title_full | Population and sex differences in Drosophila melanogaster brain gene expression |
title_fullStr | Population and sex differences in Drosophila melanogaster brain gene expression |
title_full_unstemmed | Population and sex differences in Drosophila melanogaster brain gene expression |
title_short | Population and sex differences in Drosophila melanogaster brain gene expression |
title_sort | population and sex differences in drosophila melanogaster brain gene expression |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3527002/ https://www.ncbi.nlm.nih.gov/pubmed/23170910 http://dx.doi.org/10.1186/1471-2164-13-654 |
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