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RecX Facilitates Homologous Recombination by Modulating RecA Activities
The Bacillus subtilis recH342 strain, which decreases interspecies recombination without significantly affecting the frequency of transformation with homogamic DNA, carried a point mutation in the putative recX (yfhG) gene, and the mutation was renamed as recX342. We show that RecX (264 residues lon...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3527212/ https://www.ncbi.nlm.nih.gov/pubmed/23284295 http://dx.doi.org/10.1371/journal.pgen.1003126 |
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author | Cárdenas, Paula P. Carrasco, Begoña Defeu Soufo, Clarisse César, Carolina E. Herr, Katharina Kaufenstein, Miriam Graumann, Peter L. Alonso, Juan C. |
author_facet | Cárdenas, Paula P. Carrasco, Begoña Defeu Soufo, Clarisse César, Carolina E. Herr, Katharina Kaufenstein, Miriam Graumann, Peter L. Alonso, Juan C. |
author_sort | Cárdenas, Paula P. |
collection | PubMed |
description | The Bacillus subtilis recH342 strain, which decreases interspecies recombination without significantly affecting the frequency of transformation with homogamic DNA, carried a point mutation in the putative recX (yfhG) gene, and the mutation was renamed as recX342. We show that RecX (264 residues long), which shares partial identity with the Proteobacterial RecX (<180 residues), is a genuine recombination protein, and its primary function is to modulate the SOS response and to facilitate RecA-mediated recombinational repair and genetic recombination. RecX-YFP formed discrete foci on the nucleoid, which were coincident in time with RecF, in response to DNA damage, and on the poles and/or the nucleoid upon stochastic induction of programmed natural competence. When DNA was damaged, the RecX foci co-localized with RecA threads that persisted for a longer time in the recX context. The absence of RecX severely impaired natural transformation both with plasmid and chromosomal DNA. We show that RecX suppresses the negative effect exerted by RecA during plasmid transformation, prevents RecA mis-sensing of single-stranded DNA tracts, and modulates DNA strand exchange. RecX, by modulating the “length or packing” of a RecA filament, facilitates the initiation of recombination and increases recombination across species. |
format | Online Article Text |
id | pubmed-3527212 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-35272122013-01-02 RecX Facilitates Homologous Recombination by Modulating RecA Activities Cárdenas, Paula P. Carrasco, Begoña Defeu Soufo, Clarisse César, Carolina E. Herr, Katharina Kaufenstein, Miriam Graumann, Peter L. Alonso, Juan C. PLoS Genet Research Article The Bacillus subtilis recH342 strain, which decreases interspecies recombination without significantly affecting the frequency of transformation with homogamic DNA, carried a point mutation in the putative recX (yfhG) gene, and the mutation was renamed as recX342. We show that RecX (264 residues long), which shares partial identity with the Proteobacterial RecX (<180 residues), is a genuine recombination protein, and its primary function is to modulate the SOS response and to facilitate RecA-mediated recombinational repair and genetic recombination. RecX-YFP formed discrete foci on the nucleoid, which were coincident in time with RecF, in response to DNA damage, and on the poles and/or the nucleoid upon stochastic induction of programmed natural competence. When DNA was damaged, the RecX foci co-localized with RecA threads that persisted for a longer time in the recX context. The absence of RecX severely impaired natural transformation both with plasmid and chromosomal DNA. We show that RecX suppresses the negative effect exerted by RecA during plasmid transformation, prevents RecA mis-sensing of single-stranded DNA tracts, and modulates DNA strand exchange. RecX, by modulating the “length or packing” of a RecA filament, facilitates the initiation of recombination and increases recombination across species. Public Library of Science 2012-12-20 /pmc/articles/PMC3527212/ /pubmed/23284295 http://dx.doi.org/10.1371/journal.pgen.1003126 Text en © 2012 Cárdenas et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Cárdenas, Paula P. Carrasco, Begoña Defeu Soufo, Clarisse César, Carolina E. Herr, Katharina Kaufenstein, Miriam Graumann, Peter L. Alonso, Juan C. RecX Facilitates Homologous Recombination by Modulating RecA Activities |
title | RecX Facilitates Homologous Recombination by Modulating RecA Activities |
title_full | RecX Facilitates Homologous Recombination by Modulating RecA Activities |
title_fullStr | RecX Facilitates Homologous Recombination by Modulating RecA Activities |
title_full_unstemmed | RecX Facilitates Homologous Recombination by Modulating RecA Activities |
title_short | RecX Facilitates Homologous Recombination by Modulating RecA Activities |
title_sort | recx facilitates homologous recombination by modulating reca activities |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3527212/ https://www.ncbi.nlm.nih.gov/pubmed/23284295 http://dx.doi.org/10.1371/journal.pgen.1003126 |
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