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Impact of age on the efficacy of bone marrow mononuclear cell transplantation in experimental stroke

Bone marrow-derived mononuclear cells (BM MNC) have been effectively used to treat experimental stroke. Most of the preclinical trials have been performed in young and healthy laboratory animals, even though age and hypertension are major risk factors for stroke. To determine the influence of age on...

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Autores principales: Wagner, Daniel-Christoph, Bojko, Mitja, Peters, Myriam, Lorenz, Marlene, Voigt, Cornelia, Kaminski, Alexander, Hasenclever, Dirk, Scholz, Markus, Kranz, Alexander, Weise, Gesa, Boltze, Johannes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3527344/
https://www.ncbi.nlm.nih.gov/pubmed/22920434
http://dx.doi.org/10.1186/2040-7378-4-17
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author Wagner, Daniel-Christoph
Bojko, Mitja
Peters, Myriam
Lorenz, Marlene
Voigt, Cornelia
Kaminski, Alexander
Hasenclever, Dirk
Scholz, Markus
Kranz, Alexander
Weise, Gesa
Boltze, Johannes
author_facet Wagner, Daniel-Christoph
Bojko, Mitja
Peters, Myriam
Lorenz, Marlene
Voigt, Cornelia
Kaminski, Alexander
Hasenclever, Dirk
Scholz, Markus
Kranz, Alexander
Weise, Gesa
Boltze, Johannes
author_sort Wagner, Daniel-Christoph
collection PubMed
description Bone marrow-derived mononuclear cells (BM MNC) have been effectively used to treat experimental stroke. Most of the preclinical trials have been performed in young and healthy laboratory animals, even though age and hypertension are major risk factors for stroke. To determine the influence of age on the properties of BM MNCs after cerebral ischemia, we compared the efficacy of aged and young BM MNC in an in vitro model of cerebral hypoxia and in an adapted in vivo model of stroke. Human BM MNCs were obtained from healthy young or aged donors and either co-cultured with rat hippocampal slices exposed to oxygen glucose deprivation (OGD), or transplanted intravenously 24 h after permanent middle cerebral artery occlusion in aged (18 months) spontaneously hypertensive rats (SHR). Efficacy was examined by quantification of hippocampal cell death, or respectively, by neurofunctional tests and MR investigations. Co-cultivation with young, but not with aged BM MNCs significantly reduced the hippocampal cell death after OGD. Transplantation of both young and old BM MNCs did not reduce functional deficits or ischemic lesion volume after stroke in aged SHR. These results suggest a significant impact of age on the therapeutic efficacy of BM MNCs after cerebral ischemia.
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spelling pubmed-35273442012-12-21 Impact of age on the efficacy of bone marrow mononuclear cell transplantation in experimental stroke Wagner, Daniel-Christoph Bojko, Mitja Peters, Myriam Lorenz, Marlene Voigt, Cornelia Kaminski, Alexander Hasenclever, Dirk Scholz, Markus Kranz, Alexander Weise, Gesa Boltze, Johannes Exp Transl Stroke Med Research Bone marrow-derived mononuclear cells (BM MNC) have been effectively used to treat experimental stroke. Most of the preclinical trials have been performed in young and healthy laboratory animals, even though age and hypertension are major risk factors for stroke. To determine the influence of age on the properties of BM MNCs after cerebral ischemia, we compared the efficacy of aged and young BM MNC in an in vitro model of cerebral hypoxia and in an adapted in vivo model of stroke. Human BM MNCs were obtained from healthy young or aged donors and either co-cultured with rat hippocampal slices exposed to oxygen glucose deprivation (OGD), or transplanted intravenously 24 h after permanent middle cerebral artery occlusion in aged (18 months) spontaneously hypertensive rats (SHR). Efficacy was examined by quantification of hippocampal cell death, or respectively, by neurofunctional tests and MR investigations. Co-cultivation with young, but not with aged BM MNCs significantly reduced the hippocampal cell death after OGD. Transplantation of both young and old BM MNCs did not reduce functional deficits or ischemic lesion volume after stroke in aged SHR. These results suggest a significant impact of age on the therapeutic efficacy of BM MNCs after cerebral ischemia. BioMed Central 2012-08-24 /pmc/articles/PMC3527344/ /pubmed/22920434 http://dx.doi.org/10.1186/2040-7378-4-17 Text en Copyright ©2012 Wagner et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Wagner, Daniel-Christoph
Bojko, Mitja
Peters, Myriam
Lorenz, Marlene
Voigt, Cornelia
Kaminski, Alexander
Hasenclever, Dirk
Scholz, Markus
Kranz, Alexander
Weise, Gesa
Boltze, Johannes
Impact of age on the efficacy of bone marrow mononuclear cell transplantation in experimental stroke
title Impact of age on the efficacy of bone marrow mononuclear cell transplantation in experimental stroke
title_full Impact of age on the efficacy of bone marrow mononuclear cell transplantation in experimental stroke
title_fullStr Impact of age on the efficacy of bone marrow mononuclear cell transplantation in experimental stroke
title_full_unstemmed Impact of age on the efficacy of bone marrow mononuclear cell transplantation in experimental stroke
title_short Impact of age on the efficacy of bone marrow mononuclear cell transplantation in experimental stroke
title_sort impact of age on the efficacy of bone marrow mononuclear cell transplantation in experimental stroke
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3527344/
https://www.ncbi.nlm.nih.gov/pubmed/22920434
http://dx.doi.org/10.1186/2040-7378-4-17
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