16S rRNA Terminal Restriction Fragment Length Polymorphism for the Characterization of the Nasopharyngeal Microbiota

A novel non-culture based 16S rRNA Terminal Restriction Fragment Length Polymorphism (T-RFLP) method using the restriction enzymes Tsp509I and Hpy166II was developed for the characterization of the nasopharyngeal microbiota and validated using recently published 454 pyrosequencing data. 16S rRNA gen...

Descripción completa

Detalles Bibliográficos
Autores principales: Brugger, Silvio D., Frei, Laurence, Frey, Pascal M., Aebi, Suzanne, Mühlemann, Kathrin, Hilty, Markus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3527403/
https://www.ncbi.nlm.nih.gov/pubmed/23284951
http://dx.doi.org/10.1371/journal.pone.0052241

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3527403/
https://www.ncbi.nlm.nih.gov/pubmed/23284951
http://dx.doi.org/10.1371/journal.pone.0052241

Ejemplares similares