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16S rRNA Terminal Restriction Fragment Length Polymorphism for the Characterization of the Nasopharyngeal Microbiota

A novel non-culture based 16S rRNA Terminal Restriction Fragment Length Polymorphism (T-RFLP) method using the restriction enzymes Tsp509I and Hpy166II was developed for the characterization of the nasopharyngeal microbiota and validated using recently published 454 pyrosequencing data. 16S rRNA gen...

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Detalles Bibliográficos
Autores principales:	Brugger, Silvio D., Frei, Laurence, Frey, Pascal M., Aebi, Suzanne, Mühlemann, Kathrin, Hilty, Markus
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2012
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3527403/ https://www.ncbi.nlm.nih.gov/pubmed/23284951 http://dx.doi.org/10.1371/journal.pone.0052241

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