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Bin2 Is a Membrane Sculpting N-BAR Protein That Influences Leucocyte Podosomes, Motility and Phagocytosis

Cell motility, adhesion and phagocytosis are controlled by actin and membrane remodelling processes. Bridging integrator-2 (Bin2) also called Breast cancer-associated protein 1 (BRAP1) is a predicted N-BAR domain containing protein with unknown function that is highly expressed in leucocytic cells....

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Autores principales: Sánchez-Barrena, María José, Vallis, Yvonne, Clatworthy, Menna R., Doherty, Gary J., Veprintsev, Dmitry B., Evans, Philip R., McMahon, Harvey T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3527510/
https://www.ncbi.nlm.nih.gov/pubmed/23285027
http://dx.doi.org/10.1371/journal.pone.0052401
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author Sánchez-Barrena, María José
Vallis, Yvonne
Clatworthy, Menna R.
Doherty, Gary J.
Veprintsev, Dmitry B.
Evans, Philip R.
McMahon, Harvey T.
author_facet Sánchez-Barrena, María José
Vallis, Yvonne
Clatworthy, Menna R.
Doherty, Gary J.
Veprintsev, Dmitry B.
Evans, Philip R.
McMahon, Harvey T.
author_sort Sánchez-Barrena, María José
collection PubMed
description Cell motility, adhesion and phagocytosis are controlled by actin and membrane remodelling processes. Bridging integrator-2 (Bin2) also called Breast cancer-associated protein 1 (BRAP1) is a predicted N-BAR domain containing protein with unknown function that is highly expressed in leucocytic cells. In the present study we solved the structure of Bin2 BAR domain and studied its membrane binding and bending properties in vitro and in vivo. Live-cell imaging experiments showed that Bin2 is associated with actin rich structures on the plasma membrane, where it was targeted through its N-BAR domain. Pull-down experiments and immunoprecipitations showed that Bin2 C-terminus bound SH3 domain containing proteins such as Endophilin A2 and α-PIX. siRNA of endogenous protein led to decreased cell migration, increased phagocytosis and reduced podosome density and dynamics. In contrast, overexpression of Bin2 led to decreased phagocytosis and increased podosome density and dynamics. We conclude that Bin2 is a membrane-sculpting protein that influences podosome formation, motility and phagocytosis in leucocytes. Further understanding of this protein may be key to understand the behaviour of leucocytes under physiological and pathological conditions.
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spelling pubmed-35275102013-01-02 Bin2 Is a Membrane Sculpting N-BAR Protein That Influences Leucocyte Podosomes, Motility and Phagocytosis Sánchez-Barrena, María José Vallis, Yvonne Clatworthy, Menna R. Doherty, Gary J. Veprintsev, Dmitry B. Evans, Philip R. McMahon, Harvey T. PLoS One Research Article Cell motility, adhesion and phagocytosis are controlled by actin and membrane remodelling processes. Bridging integrator-2 (Bin2) also called Breast cancer-associated protein 1 (BRAP1) is a predicted N-BAR domain containing protein with unknown function that is highly expressed in leucocytic cells. In the present study we solved the structure of Bin2 BAR domain and studied its membrane binding and bending properties in vitro and in vivo. Live-cell imaging experiments showed that Bin2 is associated with actin rich structures on the plasma membrane, where it was targeted through its N-BAR domain. Pull-down experiments and immunoprecipitations showed that Bin2 C-terminus bound SH3 domain containing proteins such as Endophilin A2 and α-PIX. siRNA of endogenous protein led to decreased cell migration, increased phagocytosis and reduced podosome density and dynamics. In contrast, overexpression of Bin2 led to decreased phagocytosis and increased podosome density and dynamics. We conclude that Bin2 is a membrane-sculpting protein that influences podosome formation, motility and phagocytosis in leucocytes. Further understanding of this protein may be key to understand the behaviour of leucocytes under physiological and pathological conditions. Public Library of Science 2012-12-20 /pmc/articles/PMC3527510/ /pubmed/23285027 http://dx.doi.org/10.1371/journal.pone.0052401 Text en © 2012 Sánchez-Barrena et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Sánchez-Barrena, María José
Vallis, Yvonne
Clatworthy, Menna R.
Doherty, Gary J.
Veprintsev, Dmitry B.
Evans, Philip R.
McMahon, Harvey T.
Bin2 Is a Membrane Sculpting N-BAR Protein That Influences Leucocyte Podosomes, Motility and Phagocytosis
title Bin2 Is a Membrane Sculpting N-BAR Protein That Influences Leucocyte Podosomes, Motility and Phagocytosis
title_full Bin2 Is a Membrane Sculpting N-BAR Protein That Influences Leucocyte Podosomes, Motility and Phagocytosis
title_fullStr Bin2 Is a Membrane Sculpting N-BAR Protein That Influences Leucocyte Podosomes, Motility and Phagocytosis
title_full_unstemmed Bin2 Is a Membrane Sculpting N-BAR Protein That Influences Leucocyte Podosomes, Motility and Phagocytosis
title_short Bin2 Is a Membrane Sculpting N-BAR Protein That Influences Leucocyte Podosomes, Motility and Phagocytosis
title_sort bin2 is a membrane sculpting n-bar protein that influences leucocyte podosomes, motility and phagocytosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3527510/
https://www.ncbi.nlm.nih.gov/pubmed/23285027
http://dx.doi.org/10.1371/journal.pone.0052401
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