Cargando…

Exine dehiscing induces rape microspore polarity, which results in different daughter cell fate and fixes the apical–basal axis of the embryo

The roles of cell polarity and the first asymmetric cell division during early embryogenesis in apical–basal cell fate determination remain unclear. Previously, a novel Brassica napus microspore embryogenesis system was established, by which rape exine-dehisced microspores were induced by physical s...

Descripción completa

Detalles Bibliográficos
Autores principales: Tang, Xingchun, Liu, Yuan, He, Yuqing, Ma, Ligang, Sun, Meng-xiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3528033/
https://www.ncbi.nlm.nih.gov/pubmed/23162119
http://dx.doi.org/10.1093/jxb/ers327
_version_ 1782253782635118592
author Tang, Xingchun
Liu, Yuan
He, Yuqing
Ma, Ligang
Sun, Meng-xiang
author_facet Tang, Xingchun
Liu, Yuan
He, Yuqing
Ma, Ligang
Sun, Meng-xiang
author_sort Tang, Xingchun
collection PubMed
description The roles of cell polarity and the first asymmetric cell division during early embryogenesis in apical–basal cell fate determination remain unclear. Previously, a novel Brassica napus microspore embryogenesis system was established, by which rape exine-dehisced microspores were induced by physical stress. Unlike traditional microspore culture, cell polarity and subsequent asymmetric division appeared in the exine-dehisced microspore, which finally developed into a typical embryo with a suspensor. Further studies indicated that polarity is critical for apical–basal cell fate determination and suspensor formation. However, the pattern of the first division was not only determined by cell polarity but was also regulated by the position of the ruptured exine. The first division could be equal or unequal, with its orientation essentially perpendicular to the polar axis. In both types of cell division, the two daughter cells could have different cell fates and give rise to an embryo with a suspensor, similar to zygotic apical–basal cell differentiation. The alignment of the two daughter cells is consistent with the orientation of the apical–basal axis of future embryonic development. Thus, the results revealed that exine dehiscing induces rape microspore polarization, and this polarity results in a different cell fate and fixes the apical–basal axis of embryogenesis, but is uncoupled from cell asymmetric division. The present study demonstrated the relationships among cell polarity, asymmetric cell division, and cell fate determination in early embryogenesis.
format Online
Article
Text
id pubmed-3528033
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-35280332012-12-21 Exine dehiscing induces rape microspore polarity, which results in different daughter cell fate and fixes the apical–basal axis of the embryo Tang, Xingchun Liu, Yuan He, Yuqing Ma, Ligang Sun, Meng-xiang J Exp Bot Research Paper The roles of cell polarity and the first asymmetric cell division during early embryogenesis in apical–basal cell fate determination remain unclear. Previously, a novel Brassica napus microspore embryogenesis system was established, by which rape exine-dehisced microspores were induced by physical stress. Unlike traditional microspore culture, cell polarity and subsequent asymmetric division appeared in the exine-dehisced microspore, which finally developed into a typical embryo with a suspensor. Further studies indicated that polarity is critical for apical–basal cell fate determination and suspensor formation. However, the pattern of the first division was not only determined by cell polarity but was also regulated by the position of the ruptured exine. The first division could be equal or unequal, with its orientation essentially perpendicular to the polar axis. In both types of cell division, the two daughter cells could have different cell fates and give rise to an embryo with a suspensor, similar to zygotic apical–basal cell differentiation. The alignment of the two daughter cells is consistent with the orientation of the apical–basal axis of future embryonic development. Thus, the results revealed that exine dehiscing induces rape microspore polarization, and this polarity results in a different cell fate and fixes the apical–basal axis of embryogenesis, but is uncoupled from cell asymmetric division. The present study demonstrated the relationships among cell polarity, asymmetric cell division, and cell fate determination in early embryogenesis. Oxford University Press 2013-01 2012-11-16 /pmc/articles/PMC3528033/ /pubmed/23162119 http://dx.doi.org/10.1093/jxb/ers327 Text en © 2012 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Tang, Xingchun
Liu, Yuan
He, Yuqing
Ma, Ligang
Sun, Meng-xiang
Exine dehiscing induces rape microspore polarity, which results in different daughter cell fate and fixes the apical–basal axis of the embryo
title Exine dehiscing induces rape microspore polarity, which results in different daughter cell fate and fixes the apical–basal axis of the embryo
title_full Exine dehiscing induces rape microspore polarity, which results in different daughter cell fate and fixes the apical–basal axis of the embryo
title_fullStr Exine dehiscing induces rape microspore polarity, which results in different daughter cell fate and fixes the apical–basal axis of the embryo
title_full_unstemmed Exine dehiscing induces rape microspore polarity, which results in different daughter cell fate and fixes the apical–basal axis of the embryo
title_short Exine dehiscing induces rape microspore polarity, which results in different daughter cell fate and fixes the apical–basal axis of the embryo
title_sort exine dehiscing induces rape microspore polarity, which results in different daughter cell fate and fixes the apical–basal axis of the embryo
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3528033/
https://www.ncbi.nlm.nih.gov/pubmed/23162119
http://dx.doi.org/10.1093/jxb/ers327
work_keys_str_mv AT tangxingchun exinedehiscinginducesrapemicrosporepolaritywhichresultsindifferentdaughtercellfateandfixestheapicalbasalaxisoftheembryo
AT liuyuan exinedehiscinginducesrapemicrosporepolaritywhichresultsindifferentdaughtercellfateandfixestheapicalbasalaxisoftheembryo
AT heyuqing exinedehiscinginducesrapemicrosporepolaritywhichresultsindifferentdaughtercellfateandfixestheapicalbasalaxisoftheembryo
AT maligang exinedehiscinginducesrapemicrosporepolaritywhichresultsindifferentdaughtercellfateandfixestheapicalbasalaxisoftheembryo
AT sunmengxiang exinedehiscinginducesrapemicrosporepolaritywhichresultsindifferentdaughtercellfateandfixestheapicalbasalaxisoftheembryo