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Evaluation of Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry for Second-Generation Lignin Analysis

Matrix-Assisted Laser Desorption/Ionization time-of-flight (MALDI-TOF) mass spectrometry is evaluated as an elucidation tool for structural features and molecular weights estimation of some extracted herbaceous lignins. Optimization of analysis conditions, using a typical organic matrix, namely α-cy...

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Autores principales: Richel, Aurore, Vanderghem, Caroline, Simon, Mathilde, Wathelet, Bernard, Paquot, Michel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Libertas Academica 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3528113/
https://www.ncbi.nlm.nih.gov/pubmed/23300342
http://dx.doi.org/10.4137/ACI.S10799
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author Richel, Aurore
Vanderghem, Caroline
Simon, Mathilde
Wathelet, Bernard
Paquot, Michel
author_facet Richel, Aurore
Vanderghem, Caroline
Simon, Mathilde
Wathelet, Bernard
Paquot, Michel
author_sort Richel, Aurore
collection PubMed
description Matrix-Assisted Laser Desorption/Ionization time-of-flight (MALDI-TOF) mass spectrometry is evaluated as an elucidation tool for structural features and molecular weights estimation of some extracted herbaceous lignins. Optimization of analysis conditions, using a typical organic matrix, namely α-cyano-4-hydroxycinnamic acid (CHCA), in combination with α-cyclodextrin, allows efficient ionization of poorly soluble lignin materials and suppression of matrix-related ions background. Analysis of low-mass fragments ions (m/z 100–600) in the positive ion mode offers a “fingerprint” of starting lignins that could be a fine strategy to qualitatively identify principal inter-unit linkages between phenylpropanoid units. The molecular weights of lignins are estimated using size exclusion chromatography and compared to MALDI-TOF-MS profiles. Miscanthus (Miscanthus x giganteus) and Switchgrass (Panicum Virgatum L.) lignins, recovered after a formic acid/acetic acid/water process or aqueous ammonia soaking, are selected as benchmarks for this study.
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spelling pubmed-35281132013-01-08 Evaluation of Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry for Second-Generation Lignin Analysis Richel, Aurore Vanderghem, Caroline Simon, Mathilde Wathelet, Bernard Paquot, Michel Anal Chem Insights Original Research Matrix-Assisted Laser Desorption/Ionization time-of-flight (MALDI-TOF) mass spectrometry is evaluated as an elucidation tool for structural features and molecular weights estimation of some extracted herbaceous lignins. Optimization of analysis conditions, using a typical organic matrix, namely α-cyano-4-hydroxycinnamic acid (CHCA), in combination with α-cyclodextrin, allows efficient ionization of poorly soluble lignin materials and suppression of matrix-related ions background. Analysis of low-mass fragments ions (m/z 100–600) in the positive ion mode offers a “fingerprint” of starting lignins that could be a fine strategy to qualitatively identify principal inter-unit linkages between phenylpropanoid units. The molecular weights of lignins are estimated using size exclusion chromatography and compared to MALDI-TOF-MS profiles. Miscanthus (Miscanthus x giganteus) and Switchgrass (Panicum Virgatum L.) lignins, recovered after a formic acid/acetic acid/water process or aqueous ammonia soaking, are selected as benchmarks for this study. Libertas Academica 2012-12-13 /pmc/articles/PMC3528113/ /pubmed/23300342 http://dx.doi.org/10.4137/ACI.S10799 Text en © 2012 the author(s), publisher and licensee Libertas Academica Ltd. This is an open access article. Unrestricted non-commercial use is permitted provided the original work is properly cited.
spellingShingle Original Research
Richel, Aurore
Vanderghem, Caroline
Simon, Mathilde
Wathelet, Bernard
Paquot, Michel
Evaluation of Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry for Second-Generation Lignin Analysis
title Evaluation of Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry for Second-Generation Lignin Analysis
title_full Evaluation of Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry for Second-Generation Lignin Analysis
title_fullStr Evaluation of Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry for Second-Generation Lignin Analysis
title_full_unstemmed Evaluation of Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry for Second-Generation Lignin Analysis
title_short Evaluation of Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry for Second-Generation Lignin Analysis
title_sort evaluation of matrix-assisted laser desorption/ionization mass spectrometry for second-generation lignin analysis
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3528113/
https://www.ncbi.nlm.nih.gov/pubmed/23300342
http://dx.doi.org/10.4137/ACI.S10799
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