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Promoter Trapping in Microalgae Using the Antibiotic Paromomycin as Selective Agent

The lack of highly active endogenous promoters to drive the expression of transgenes is one of the main drawbacks to achieving efficient transformation of many microalgal species. Using the model chlorophyte Chlamydomonas reinhardtii and the paromomycin resistance APHVIII gene from Streptomyces rimo...

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Detalles Bibliográficos
Autores principales: Vila, Marta, Díaz-Santos, Encarnación, de la, Marta, Rodríguez, Herminia, Vargas, Ángeles, León, Rosa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3528124/
https://www.ncbi.nlm.nih.gov/pubmed/23211713
http://dx.doi.org/10.3390/md10122749