Chimeric β-Lactamases: Global Conservation of Parental Function and Fast Time-Scale Dynamics with Increased Slow Motions

Enzyme engineering has been facilitated by recombination of close homologues, followed by functional screening. In one such effort, chimeras of two class-A β-lactamases – TEM-1 and PSE-4 – were created according to structure-guided protein recombination and selected for their capacity to promote bac...

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Detalles Bibliográficos
Autores principales: Clouthier, Christopher M., Morin, Sébastien, Gobeil, Sophie M. C., Doucet, Nicolas, Blanchet, Jonathan, Nguyen, Elisabeth, Gagné, Stéphane M., Pelletier, Joelle N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3528772/
https://www.ncbi.nlm.nih.gov/pubmed/23284969
http://dx.doi.org/10.1371/journal.pone.0052283

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