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Correlation between three assay systems for anti-Müllerian hormone (AMH) determination
PURPOSE: Analysis of anti-Müllerian hormone (AMH) is becoming of recognized importance in reproductive medicine, but assays are not standardized. We have evaluated the correlation between the new Gen II ELISA kit (Beckman-Coutler) and the older ELISA kits by Immunotech (IOT) and Diagnostic Systems L...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3528883/ https://www.ncbi.nlm.nih.gov/pubmed/23117477 http://dx.doi.org/10.1007/s10815-012-9880-1 |
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author | Li, Hang Wun Raymond Ng, Ernest Hung Yu Wong, Benancy Po Chau Anderson, Richard A. Ho, Pak Chung Yeung, William Shu Biu |
author_facet | Li, Hang Wun Raymond Ng, Ernest Hung Yu Wong, Benancy Po Chau Anderson, Richard A. Ho, Pak Chung Yeung, William Shu Biu |
author_sort | Li, Hang Wun Raymond |
collection | PubMed |
description | PURPOSE: Analysis of anti-Müllerian hormone (AMH) is becoming of recognized importance in reproductive medicine, but assays are not standardized. We have evaluated the correlation between the new Gen II ELISA kit (Beckman-Coutler) and the older ELISA kits by Immunotech (IOT) and Diagnostic Systems Laboratories (DSL). METHODS: A total of 56 archived serum samples from patients with subfertility or reproductive endocrine disorders were retrieved and assayed in duplicate using the three AMH ELISA kits . The samples covered a wide range of AMH concentrations (1.9 to 142.5 pmol/L). RESULTS: We observed good correlations between the new (AMH Gen II) and old AMH assay kits by IOT and DSL (R(2) = 0.971 and 0.930 respectively). The regression equations were AMH (Gen II) = 1.353 × AMH (IOT) + 0.051 and AMH (Gen II) = 1.223 × AMH (DSL) – 1.270 respectively. CONCLUSIONS: AMH concentrations using the Gen II kit are slightly higher than those from the IOT and DSL kits. Standardization of assay results worldwide is urgently required but this analysis facilitates the interpretation of values obtained historically and in future studies using any of the 3 assays available. Meanwhile, adapting clinical cut-offs from previously published work by direct conversion is not recommended. |
format | Online Article Text |
id | pubmed-3528883 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-35288832013-01-04 Correlation between three assay systems for anti-Müllerian hormone (AMH) determination Li, Hang Wun Raymond Ng, Ernest Hung Yu Wong, Benancy Po Chau Anderson, Richard A. Ho, Pak Chung Yeung, William Shu Biu J Assist Reprod Genet Technological Innovations PURPOSE: Analysis of anti-Müllerian hormone (AMH) is becoming of recognized importance in reproductive medicine, but assays are not standardized. We have evaluated the correlation between the new Gen II ELISA kit (Beckman-Coutler) and the older ELISA kits by Immunotech (IOT) and Diagnostic Systems Laboratories (DSL). METHODS: A total of 56 archived serum samples from patients with subfertility or reproductive endocrine disorders were retrieved and assayed in duplicate using the three AMH ELISA kits . The samples covered a wide range of AMH concentrations (1.9 to 142.5 pmol/L). RESULTS: We observed good correlations between the new (AMH Gen II) and old AMH assay kits by IOT and DSL (R(2) = 0.971 and 0.930 respectively). The regression equations were AMH (Gen II) = 1.353 × AMH (IOT) + 0.051 and AMH (Gen II) = 1.223 × AMH (DSL) – 1.270 respectively. CONCLUSIONS: AMH concentrations using the Gen II kit are slightly higher than those from the IOT and DSL kits. Standardization of assay results worldwide is urgently required but this analysis facilitates the interpretation of values obtained historically and in future studies using any of the 3 assays available. Meanwhile, adapting clinical cut-offs from previously published work by direct conversion is not recommended. Springer US 2012-11-02 2012-12 /pmc/articles/PMC3528883/ /pubmed/23117477 http://dx.doi.org/10.1007/s10815-012-9880-1 Text en © The Author(s) 2012 https://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. |
spellingShingle | Technological Innovations Li, Hang Wun Raymond Ng, Ernest Hung Yu Wong, Benancy Po Chau Anderson, Richard A. Ho, Pak Chung Yeung, William Shu Biu Correlation between three assay systems for anti-Müllerian hormone (AMH) determination |
title | Correlation between three assay systems for anti-Müllerian hormone (AMH) determination |
title_full | Correlation between three assay systems for anti-Müllerian hormone (AMH) determination |
title_fullStr | Correlation between three assay systems for anti-Müllerian hormone (AMH) determination |
title_full_unstemmed | Correlation between three assay systems for anti-Müllerian hormone (AMH) determination |
title_short | Correlation between three assay systems for anti-Müllerian hormone (AMH) determination |
title_sort | correlation between three assay systems for anti-müllerian hormone (amh) determination |
topic | Technological Innovations |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3528883/ https://www.ncbi.nlm.nih.gov/pubmed/23117477 http://dx.doi.org/10.1007/s10815-012-9880-1 |
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