An Early Morning Sputum Sample Is Necessary for the Diagnosis of Pulmonary Tuberculosis, Even with More Sensitive Techniques: A Prospective Cohort Study among Adolescent TB-Suspects in Uganda

The World Health Organization (WHO) recommends collection of two sputum samples for tuberculosis (TB) diagnosis, with at least one being an early morning (EM) using smear microscopy. It remains unclear whether this is necessary even when sputum culture is employed. Here, we determined the diagnostic...

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Autores principales: Ssengooba, Willy, Kateete, David P., Wajja, Anne, Bugumirwa, Eric, Mboowa, Gerald, Namaganda, Carolyn, Nakayita, Germine, Nassolo, Maria, Mumbowa, Francis, Asiimwe, Benon B., Waako, James, Verver, Suzanne, Musoke, Philippa, Mayanja-Kizza, Harriet, Joloba, Moses L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Clinical Study
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3529437/
https://www.ncbi.nlm.nih.gov/pubmed/23304491
http://dx.doi.org/10.1155/2012/970203
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author Ssengooba, Willy
Kateete, David P.
Wajja, Anne
Bugumirwa, Eric
Mboowa, Gerald
Namaganda, Carolyn
Nakayita, Germine
Nassolo, Maria
Mumbowa, Francis
Asiimwe, Benon B.
Waako, James
Verver, Suzanne
Musoke, Philippa
Mayanja-Kizza, Harriet
Joloba, Moses L.
author_facet Ssengooba, Willy
Kateete, David P.
Wajja, Anne
Bugumirwa, Eric
Mboowa, Gerald
Namaganda, Carolyn
Nakayita, Germine
Nassolo, Maria
Mumbowa, Francis
Asiimwe, Benon B.
Waako, James
Verver, Suzanne
Musoke, Philippa
Mayanja-Kizza, Harriet
Joloba, Moses L.
author_sort Ssengooba, Willy
collection PubMed
description The World Health Organization (WHO) recommends collection of two sputum samples for tuberculosis (TB) diagnosis, with at least one being an early morning (EM) using smear microscopy. It remains unclear whether this is necessary even when sputum culture is employed. Here, we determined the diagnostic yield from spot and the incremental yield from the EM sputum sample cultures among TB-suspected adolescents from rural Uganda. Sputum samples (both spot and early-morning) from 1862 adolescents were cultured by the Lowenstein-Jensen (LJ) and Mycobacterium Growth Indicator Tube (MGIT) methods. For spot samples, the diagnostic yields for TB were 19.0% and 57.1% with LJ and MGIT, respectively, whereas the incremental yields (not totals) of the early-morning sample were 9.5% and 42.9% (P < 0.001) with LJ and MGIT, respectively. Among TB-suspected adolescents in rural Uganda, the EM sputum culture has a high incremental diagnostic yield. Therefore, EM sputum in addition to spot sample culture is necessary for improved TB case detection.
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spelling pubmed-35294372013-01-09 An Early Morning Sputum Sample Is Necessary for the Diagnosis of Pulmonary Tuberculosis, Even with More Sensitive Techniques: A Prospective Cohort Study among Adolescent TB-Suspects in Uganda Ssengooba, Willy Kateete, David P. Wajja, Anne Bugumirwa, Eric Mboowa, Gerald Namaganda, Carolyn Nakayita, Germine Nassolo, Maria Mumbowa, Francis Asiimwe, Benon B. Waako, James Verver, Suzanne Musoke, Philippa Mayanja-Kizza, Harriet Joloba, Moses L. Tuberc Res Treat Clinical Study The World Health Organization (WHO) recommends collection of two sputum samples for tuberculosis (TB) diagnosis, with at least one being an early morning (EM) using smear microscopy. It remains unclear whether this is necessary even when sputum culture is employed. Here, we determined the diagnostic yield from spot and the incremental yield from the EM sputum sample cultures among TB-suspected adolescents from rural Uganda. Sputum samples (both spot and early-morning) from 1862 adolescents were cultured by the Lowenstein-Jensen (LJ) and Mycobacterium Growth Indicator Tube (MGIT) methods. For spot samples, the diagnostic yields for TB were 19.0% and 57.1% with LJ and MGIT, respectively, whereas the incremental yields (not totals) of the early-morning sample were 9.5% and 42.9% (P < 0.001) with LJ and MGIT, respectively. Among TB-suspected adolescents in rural Uganda, the EM sputum culture has a high incremental diagnostic yield. Therefore, EM sputum in addition to spot sample culture is necessary for improved TB case detection. Hindawi Publishing Corporation 2012 2012-12-04 /pmc/articles/PMC3529437/ /pubmed/23304491 http://dx.doi.org/10.1155/2012/970203 Text en Copyright © 2012 Willy Ssengooba et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Clinical Study
Ssengooba, Willy
Kateete, David P.
Wajja, Anne
Bugumirwa, Eric
Mboowa, Gerald
Namaganda, Carolyn
Nakayita, Germine
Nassolo, Maria
Mumbowa, Francis
Asiimwe, Benon B.
Waako, James
Verver, Suzanne
Musoke, Philippa
Mayanja-Kizza, Harriet
Joloba, Moses L.
An Early Morning Sputum Sample Is Necessary for the Diagnosis of Pulmonary Tuberculosis, Even with More Sensitive Techniques: A Prospective Cohort Study among Adolescent TB-Suspects in Uganda
title An Early Morning Sputum Sample Is Necessary for the Diagnosis of Pulmonary Tuberculosis, Even with More Sensitive Techniques: A Prospective Cohort Study among Adolescent TB-Suspects in Uganda
title_full An Early Morning Sputum Sample Is Necessary for the Diagnosis of Pulmonary Tuberculosis, Even with More Sensitive Techniques: A Prospective Cohort Study among Adolescent TB-Suspects in Uganda
title_fullStr An Early Morning Sputum Sample Is Necessary for the Diagnosis of Pulmonary Tuberculosis, Even with More Sensitive Techniques: A Prospective Cohort Study among Adolescent TB-Suspects in Uganda
title_full_unstemmed An Early Morning Sputum Sample Is Necessary for the Diagnosis of Pulmonary Tuberculosis, Even with More Sensitive Techniques: A Prospective Cohort Study among Adolescent TB-Suspects in Uganda
title_short An Early Morning Sputum Sample Is Necessary for the Diagnosis of Pulmonary Tuberculosis, Even with More Sensitive Techniques: A Prospective Cohort Study among Adolescent TB-Suspects in Uganda
title_sort early morning sputum sample is necessary for the diagnosis of pulmonary tuberculosis, even with more sensitive techniques: a prospective cohort study among adolescent tb-suspects in uganda
topic Clinical Study
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3529437/
https://www.ncbi.nlm.nih.gov/pubmed/23304491
http://dx.doi.org/10.1155/2012/970203
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