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Chronic Subordinate Colony Housing (CSC) as a Model of Chronic Psychosocial Stress in Male Rats

Chronic subordinate colony housing (CSC) is an adequate and reliable mouse model of chronic psychosocial stress, resulting in reduced body weight gain, reduced thymus and increased adrenal weight, long-lasting anxiety-like behaviour, and spontaneous colitis. Furthermore, CSC mice show increased cort...

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Autores principales: Nyuyki, Kewir D., Beiderbeck, Daniela I., Lukas, Michael, Neumann, Inga D., Reber, Stefan O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3530595/
https://www.ncbi.nlm.nih.gov/pubmed/23300653
http://dx.doi.org/10.1371/journal.pone.0052371
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author Nyuyki, Kewir D.
Beiderbeck, Daniela I.
Lukas, Michael
Neumann, Inga D.
Reber, Stefan O.
author_facet Nyuyki, Kewir D.
Beiderbeck, Daniela I.
Lukas, Michael
Neumann, Inga D.
Reber, Stefan O.
author_sort Nyuyki, Kewir D.
collection PubMed
description Chronic subordinate colony housing (CSC) is an adequate and reliable mouse model of chronic psychosocial stress, resulting in reduced body weight gain, reduced thymus and increased adrenal weight, long-lasting anxiety-like behaviour, and spontaneous colitis. Furthermore, CSC mice show increased corticotrophin (ACTH) responsiveness to acute heterotypic stressors, suggesting a general mechanism which allows a chronically-stressed organism to adequately respond to a novel threat. Therefore, the aim of the present study was to extend the CSC model to another rodent species, namely male Wistar rats, and to characterize relevant physiological, immunological, and behavioural consequences; placing particular emphasis on changes in hypothalamo-pituitary-adrenal (HPA) axis responsiveness to an acute heterotypic stressor. In line with previous mouse data, exposure of Wistar rats to 19 days of CSC resulted in a decrease in body weight gain and absolute thymus mass, mild colonic barrier defects and intestinal immune activation. Moreover, no changes in stress-coping behaviour or social preference were seen; again in agreement with the mouse paradigm. Most importantly, CSC rats showed an increased plasma corticosterone response to an acute heterotypic stressor (open arm, 5 min) despite displaying similar basal levels and similar basal and stressor-induced plasma ACTH levels. In contrast to CSC mice, anxiety-related behaviour and absolute, as well as relative adrenal weights remained unchanged in CSC rats. In summary, the CSC paradigm could be established as an adequate model of chronic psychosocial stress in male rats. Our data further support the initial hypothesis that adrenal hyper-responsiveness to ACTH during acute heterotypic stressors represents a general adaptation, which enables a chronically-stressed organism to adequately respond to novel challenges.
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spelling pubmed-35305952013-01-08 Chronic Subordinate Colony Housing (CSC) as a Model of Chronic Psychosocial Stress in Male Rats Nyuyki, Kewir D. Beiderbeck, Daniela I. Lukas, Michael Neumann, Inga D. Reber, Stefan O. PLoS One Research Article Chronic subordinate colony housing (CSC) is an adequate and reliable mouse model of chronic psychosocial stress, resulting in reduced body weight gain, reduced thymus and increased adrenal weight, long-lasting anxiety-like behaviour, and spontaneous colitis. Furthermore, CSC mice show increased corticotrophin (ACTH) responsiveness to acute heterotypic stressors, suggesting a general mechanism which allows a chronically-stressed organism to adequately respond to a novel threat. Therefore, the aim of the present study was to extend the CSC model to another rodent species, namely male Wistar rats, and to characterize relevant physiological, immunological, and behavioural consequences; placing particular emphasis on changes in hypothalamo-pituitary-adrenal (HPA) axis responsiveness to an acute heterotypic stressor. In line with previous mouse data, exposure of Wistar rats to 19 days of CSC resulted in a decrease in body weight gain and absolute thymus mass, mild colonic barrier defects and intestinal immune activation. Moreover, no changes in stress-coping behaviour or social preference were seen; again in agreement with the mouse paradigm. Most importantly, CSC rats showed an increased plasma corticosterone response to an acute heterotypic stressor (open arm, 5 min) despite displaying similar basal levels and similar basal and stressor-induced plasma ACTH levels. In contrast to CSC mice, anxiety-related behaviour and absolute, as well as relative adrenal weights remained unchanged in CSC rats. In summary, the CSC paradigm could be established as an adequate model of chronic psychosocial stress in male rats. Our data further support the initial hypothesis that adrenal hyper-responsiveness to ACTH during acute heterotypic stressors represents a general adaptation, which enables a chronically-stressed organism to adequately respond to novel challenges. Public Library of Science 2012-12-26 /pmc/articles/PMC3530595/ /pubmed/23300653 http://dx.doi.org/10.1371/journal.pone.0052371 Text en © 2012 Nyuyki et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Nyuyki, Kewir D.
Beiderbeck, Daniela I.
Lukas, Michael
Neumann, Inga D.
Reber, Stefan O.
Chronic Subordinate Colony Housing (CSC) as a Model of Chronic Psychosocial Stress in Male Rats
title Chronic Subordinate Colony Housing (CSC) as a Model of Chronic Psychosocial Stress in Male Rats
title_full Chronic Subordinate Colony Housing (CSC) as a Model of Chronic Psychosocial Stress in Male Rats
title_fullStr Chronic Subordinate Colony Housing (CSC) as a Model of Chronic Psychosocial Stress in Male Rats
title_full_unstemmed Chronic Subordinate Colony Housing (CSC) as a Model of Chronic Psychosocial Stress in Male Rats
title_short Chronic Subordinate Colony Housing (CSC) as a Model of Chronic Psychosocial Stress in Male Rats
title_sort chronic subordinate colony housing (csc) as a model of chronic psychosocial stress in male rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3530595/
https://www.ncbi.nlm.nih.gov/pubmed/23300653
http://dx.doi.org/10.1371/journal.pone.0052371
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