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Induction of cytokines in different organs after intranasal inoculation of Campylobacter jejuni in mice
BACKGROUND: Cytokine production and histopathological changes occur in the lungs of mice after intranasal inoculation with Campylobacter jejuni, but the levels of cytokines in different organs to which C. jejuni disseminates have not been studied. FINDINGS: Adult BALB/c mice were intranasally inocul...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3531258/ https://www.ncbi.nlm.nih.gov/pubmed/23244595 http://dx.doi.org/10.1186/1757-4749-4-23 |
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author | Al-Banna, Nadia A Raghupathy, Raj Albert, M John |
author_facet | Al-Banna, Nadia A Raghupathy, Raj Albert, M John |
author_sort | Al-Banna, Nadia A |
collection | PubMed |
description | BACKGROUND: Cytokine production and histopathological changes occur in the lungs of mice after intranasal inoculation with Campylobacter jejuni, but the levels of cytokines in different organs to which C. jejuni disseminates have not been studied. FINDINGS: Adult BALB/c mice were intranasally inoculated with C. jejuni 81–176 (test) or phosphate-buffered saline (control) (n=16 per group). The levels of cytokines in the organs (spleen, liver, and small and large intestines) to which C. jejuni disseminated were measured by ELISA. Two cytokine patterns were observed. First, increased proinflammatory cytokines, TNF-α, IL-1, and IL-2, were followed by anti-inflammatory cytokines, IL-4 and IL-10 in the spleen and large intestine. Second, in the liver and small intestine, there was a predominant production of anti-inflammatory cytokines, IL-4 and IL-10, with some increase in IL-2 levels. In the spleen and intestines, the levels of pro- and anti-inflammatory cytokines were concurrently increased. CONCLUSION: Dissemination of C. jejuni is associated with the production of different cytokine profiles in different tissues, with the proinflammatory response appearing in the spleen and large intestine at an earlier time point than in the liver and small intestine. The organs produce different cytokine profiles in response to C. jejuni dissemination. These preliminary findings should be confirmed with a study involving a larger group of animals. |
format | Online Article Text |
id | pubmed-3531258 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-35312582013-01-10 Induction of cytokines in different organs after intranasal inoculation of Campylobacter jejuni in mice Al-Banna, Nadia A Raghupathy, Raj Albert, M John Gut Pathog Short Report BACKGROUND: Cytokine production and histopathological changes occur in the lungs of mice after intranasal inoculation with Campylobacter jejuni, but the levels of cytokines in different organs to which C. jejuni disseminates have not been studied. FINDINGS: Adult BALB/c mice were intranasally inoculated with C. jejuni 81–176 (test) or phosphate-buffered saline (control) (n=16 per group). The levels of cytokines in the organs (spleen, liver, and small and large intestines) to which C. jejuni disseminated were measured by ELISA. Two cytokine patterns were observed. First, increased proinflammatory cytokines, TNF-α, IL-1, and IL-2, were followed by anti-inflammatory cytokines, IL-4 and IL-10 in the spleen and large intestine. Second, in the liver and small intestine, there was a predominant production of anti-inflammatory cytokines, IL-4 and IL-10, with some increase in IL-2 levels. In the spleen and intestines, the levels of pro- and anti-inflammatory cytokines were concurrently increased. CONCLUSION: Dissemination of C. jejuni is associated with the production of different cytokine profiles in different tissues, with the proinflammatory response appearing in the spleen and large intestine at an earlier time point than in the liver and small intestine. The organs produce different cytokine profiles in response to C. jejuni dissemination. These preliminary findings should be confirmed with a study involving a larger group of animals. BioMed Central 2012-12-17 /pmc/articles/PMC3531258/ /pubmed/23244595 http://dx.doi.org/10.1186/1757-4749-4-23 Text en Copyright ©2012 Al-Banna et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Report Al-Banna, Nadia A Raghupathy, Raj Albert, M John Induction of cytokines in different organs after intranasal inoculation of Campylobacter jejuni in mice |
title | Induction of cytokines in different organs after intranasal inoculation of Campylobacter jejuni in mice |
title_full | Induction of cytokines in different organs after intranasal inoculation of Campylobacter jejuni in mice |
title_fullStr | Induction of cytokines in different organs after intranasal inoculation of Campylobacter jejuni in mice |
title_full_unstemmed | Induction of cytokines in different organs after intranasal inoculation of Campylobacter jejuni in mice |
title_short | Induction of cytokines in different organs after intranasal inoculation of Campylobacter jejuni in mice |
title_sort | induction of cytokines in different organs after intranasal inoculation of campylobacter jejuni in mice |
topic | Short Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3531258/ https://www.ncbi.nlm.nih.gov/pubmed/23244595 http://dx.doi.org/10.1186/1757-4749-4-23 |
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