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Short Term Interactions with Long Term Consequences: Modulation of Chimeric Vessels by Neural Progenitors

Vessels are a critical and necessary component of most tissues, and there has been substantial research investigating vessel formation and stabilization. Several groups have investigated coculturing endothelial cells with a second cell type to promote formation and stabilization of vessels. Some hav...

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Autores principales: Williams, Cicely, Rauch, Millicent Ford, Michaud, Michael, Robinson, Rebecca, Xu, Hao, Madri, Joseph, Lavik, Erin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3531360/
https://www.ncbi.nlm.nih.gov/pubmed/23300890
http://dx.doi.org/10.1371/journal.pone.0053208
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author Williams, Cicely
Rauch, Millicent Ford
Michaud, Michael
Robinson, Rebecca
Xu, Hao
Madri, Joseph
Lavik, Erin
author_facet Williams, Cicely
Rauch, Millicent Ford
Michaud, Michael
Robinson, Rebecca
Xu, Hao
Madri, Joseph
Lavik, Erin
author_sort Williams, Cicely
collection PubMed
description Vessels are a critical and necessary component of most tissues, and there has been substantial research investigating vessel formation and stabilization. Several groups have investigated coculturing endothelial cells with a second cell type to promote formation and stabilization of vessels. Some have noted that long-term vessels derived from implanted cocultures are often chimeric consisting of both host and donor cells. The questions arise as to whether the coculture cell might impact the chimeric nature of the microvessels and can modulate the density of donor cells over time. If long-term engineered microvessels are primarily of host origin, any impairment of the host's angiogenic ability has significant implications for the long-term success of the implant. If one can modulate the host versus donor response, one may be able to overcome a host's angiogenic impairment. Furthermore, if one can modulate the donor contribution, one may be able to engineer microvascular networks to deliver molecules a patient lacks systemically for long times. To investigate the impact of the cocultured cell on the host versus donor contributions of endothelial cells in engineered microvascular networks, we varied the ratio of the neural progenitors to endothelial cells in subcutaneously implanted poly(ethylene glycol)/poly-L-lysine hydrogels. We found that the coculture of neural progenitors with endothelial cells led to the formation of chimeric host-donor vessels, and the ratio of neural progenitors has a significant impact on the long term residence of donor endothelial cells in engineered microvascular networks in vivo even though the neural progenitors are only present transiently in the system. We attribute this to the short term paracrine signaling between the two cell types. This suggests that one can modulate the host versus donor contributions using short-term paracrine signaling which has broad implications for the application of engineered microvascular networks and cellular therapy more broadly.
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spelling pubmed-35313602013-01-08 Short Term Interactions with Long Term Consequences: Modulation of Chimeric Vessels by Neural Progenitors Williams, Cicely Rauch, Millicent Ford Michaud, Michael Robinson, Rebecca Xu, Hao Madri, Joseph Lavik, Erin PLoS One Research Article Vessels are a critical and necessary component of most tissues, and there has been substantial research investigating vessel formation and stabilization. Several groups have investigated coculturing endothelial cells with a second cell type to promote formation and stabilization of vessels. Some have noted that long-term vessels derived from implanted cocultures are often chimeric consisting of both host and donor cells. The questions arise as to whether the coculture cell might impact the chimeric nature of the microvessels and can modulate the density of donor cells over time. If long-term engineered microvessels are primarily of host origin, any impairment of the host's angiogenic ability has significant implications for the long-term success of the implant. If one can modulate the host versus donor response, one may be able to overcome a host's angiogenic impairment. Furthermore, if one can modulate the donor contribution, one may be able to engineer microvascular networks to deliver molecules a patient lacks systemically for long times. To investigate the impact of the cocultured cell on the host versus donor contributions of endothelial cells in engineered microvascular networks, we varied the ratio of the neural progenitors to endothelial cells in subcutaneously implanted poly(ethylene glycol)/poly-L-lysine hydrogels. We found that the coculture of neural progenitors with endothelial cells led to the formation of chimeric host-donor vessels, and the ratio of neural progenitors has a significant impact on the long term residence of donor endothelial cells in engineered microvascular networks in vivo even though the neural progenitors are only present transiently in the system. We attribute this to the short term paracrine signaling between the two cell types. This suggests that one can modulate the host versus donor contributions using short-term paracrine signaling which has broad implications for the application of engineered microvascular networks and cellular therapy more broadly. Public Library of Science 2012-12-27 /pmc/articles/PMC3531360/ /pubmed/23300890 http://dx.doi.org/10.1371/journal.pone.0053208 Text en © 2012 Williams et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Williams, Cicely
Rauch, Millicent Ford
Michaud, Michael
Robinson, Rebecca
Xu, Hao
Madri, Joseph
Lavik, Erin
Short Term Interactions with Long Term Consequences: Modulation of Chimeric Vessels by Neural Progenitors
title Short Term Interactions with Long Term Consequences: Modulation of Chimeric Vessels by Neural Progenitors
title_full Short Term Interactions with Long Term Consequences: Modulation of Chimeric Vessels by Neural Progenitors
title_fullStr Short Term Interactions with Long Term Consequences: Modulation of Chimeric Vessels by Neural Progenitors
title_full_unstemmed Short Term Interactions with Long Term Consequences: Modulation of Chimeric Vessels by Neural Progenitors
title_short Short Term Interactions with Long Term Consequences: Modulation of Chimeric Vessels by Neural Progenitors
title_sort short term interactions with long term consequences: modulation of chimeric vessels by neural progenitors
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3531360/
https://www.ncbi.nlm.nih.gov/pubmed/23300890
http://dx.doi.org/10.1371/journal.pone.0053208
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