Interaction of Salmonella Typhimurium with Dendritic Cells Derived from Pluripotent Embryonic Stem Cells

Using an in vitro differentiation protocol we isolated cells with the properties of dendritic cells (DCs) from immunologically refractive pluripotent murine embryonic stem cells (ESCs). These ES-derived dendritic cells (ESDCs) expressed cytokines and were able to present antigen to a T cell line. In...

Descripción completa

Detalles Bibliográficos
Autores principales: Rossi, Raffaella, Hale, Christine, Goulding, David, Andrews, Robert, Abdellah, Zarah, Fairchild, Paul J., Dougan, Gordon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3532158/
https://www.ncbi.nlm.nih.gov/pubmed/23284947
http://dx.doi.org/10.1371/journal.pone.0052232
_version_ 1782254261628829696
author Rossi, Raffaella
Hale, Christine
Goulding, David
Andrews, Robert
Abdellah, Zarah
Fairchild, Paul J.
Dougan, Gordon
author_facet Rossi, Raffaella
Hale, Christine
Goulding, David
Andrews, Robert
Abdellah, Zarah
Fairchild, Paul J.
Dougan, Gordon
author_sort Rossi, Raffaella
collection PubMed
description Using an in vitro differentiation protocol we isolated cells with the properties of dendritic cells (DCs) from immunologically refractive pluripotent murine embryonic stem cells (ESCs). These ES-derived dendritic cells (ESDCs) expressed cytokines and were able to present antigen to a T cell line. Infection of ESDCs with Salmonella Typhimurium stimulated the expression of immune cell markers and thousands of murine genes, many associated with the immune response. Consequently, this system provides a novel in vitro model, amenable to genetic modification, for monitoring host/pathogen interactions.
format Online
Article
Text
id pubmed-3532158
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-35321582013-01-02 Interaction of Salmonella Typhimurium with Dendritic Cells Derived from Pluripotent Embryonic Stem Cells Rossi, Raffaella Hale, Christine Goulding, David Andrews, Robert Abdellah, Zarah Fairchild, Paul J. Dougan, Gordon PLoS One Research Article Using an in vitro differentiation protocol we isolated cells with the properties of dendritic cells (DCs) from immunologically refractive pluripotent murine embryonic stem cells (ESCs). These ES-derived dendritic cells (ESDCs) expressed cytokines and were able to present antigen to a T cell line. Infection of ESDCs with Salmonella Typhimurium stimulated the expression of immune cell markers and thousands of murine genes, many associated with the immune response. Consequently, this system provides a novel in vitro model, amenable to genetic modification, for monitoring host/pathogen interactions. Public Library of Science 2012-12-28 /pmc/articles/PMC3532158/ /pubmed/23284947 http://dx.doi.org/10.1371/journal.pone.0052232 Text en © 2012 Rossi et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Rossi, Raffaella
Hale, Christine
Goulding, David
Andrews, Robert
Abdellah, Zarah
Fairchild, Paul J.
Dougan, Gordon
Interaction of Salmonella Typhimurium with Dendritic Cells Derived from Pluripotent Embryonic Stem Cells
title Interaction of Salmonella Typhimurium with Dendritic Cells Derived from Pluripotent Embryonic Stem Cells
title_full Interaction of Salmonella Typhimurium with Dendritic Cells Derived from Pluripotent Embryonic Stem Cells
title_fullStr Interaction of Salmonella Typhimurium with Dendritic Cells Derived from Pluripotent Embryonic Stem Cells
title_full_unstemmed Interaction of Salmonella Typhimurium with Dendritic Cells Derived from Pluripotent Embryonic Stem Cells
title_short Interaction of Salmonella Typhimurium with Dendritic Cells Derived from Pluripotent Embryonic Stem Cells
title_sort interaction of salmonella typhimurium with dendritic cells derived from pluripotent embryonic stem cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3532158/
https://www.ncbi.nlm.nih.gov/pubmed/23284947
http://dx.doi.org/10.1371/journal.pone.0052232
work_keys_str_mv AT rossiraffaella interactionofsalmonellatyphimuriumwithdendriticcellsderivedfrompluripotentembryonicstemcells
AT halechristine interactionofsalmonellatyphimuriumwithdendriticcellsderivedfrompluripotentembryonicstemcells
AT gouldingdavid interactionofsalmonellatyphimuriumwithdendriticcellsderivedfrompluripotentembryonicstemcells
AT andrewsrobert interactionofsalmonellatyphimuriumwithdendriticcellsderivedfrompluripotentembryonicstemcells
AT abdellahzarah interactionofsalmonellatyphimuriumwithdendriticcellsderivedfrompluripotentembryonicstemcells
AT fairchildpaulj interactionofsalmonellatyphimuriumwithdendriticcellsderivedfrompluripotentembryonicstemcells
AT dougangordon interactionofsalmonellatyphimuriumwithdendriticcellsderivedfrompluripotentembryonicstemcells

Ejemplares similares