Cargando…
A combination of intradermal jet-injection and electroporation overcomes in vivo dose restriction of DNA vaccines
BACKGROUND: The use of optimized delivery devices has been shown to enhance the potency of DNA vaccines. However, further optimization of DNA vaccine delivery is needed for this vaccine modality to ultimately be efficacious in humans. METHODS: Herein we evaluated antigen expression and immunogenicit...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3532290/ https://www.ncbi.nlm.nih.gov/pubmed/22873174 http://dx.doi.org/10.1186/1479-0556-10-5 |
_version_ | 1782254281352544256 |
---|---|
author | Hallengärd, David Bråve, Andreas Isaguliants, Maria Blomberg, Pontus Enger, Jenny Stout, Richard King, Alan Wahren, Britta |
author_facet | Hallengärd, David Bråve, Andreas Isaguliants, Maria Blomberg, Pontus Enger, Jenny Stout, Richard King, Alan Wahren, Britta |
author_sort | Hallengärd, David |
collection | PubMed |
description | BACKGROUND: The use of optimized delivery devices has been shown to enhance the potency of DNA vaccines. However, further optimization of DNA vaccine delivery is needed for this vaccine modality to ultimately be efficacious in humans. METHODS: Herein we evaluated antigen expression and immunogenicity after intradermal delivery of different doses of DNA vaccines by needle or by the Biojector jet-injection device, with or without the addition of electroporation (EP). RESULTS: Neither needle injection augmented by EP nor Biojector alone could induce higher magnitudes of immune responses after immunizations with a high dose of DNA. After division of a defined DNA dose into multiple skin sites, the humoral response was particularly enhanced by Biojector while cellular responses were particularly enhanced by EP. Furthermore, a close correlation between in vivo antigen expression and cell-mediated as well as humoral immune responses was observed. CONCLUSIONS: These results show that two optimized DNA vaccine delivery devices can act together to overcome dose restrictions of plasmid DNA vaccines. |
format | Online Article Text |
id | pubmed-3532290 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-35322902013-01-03 A combination of intradermal jet-injection and electroporation overcomes in vivo dose restriction of DNA vaccines Hallengärd, David Bråve, Andreas Isaguliants, Maria Blomberg, Pontus Enger, Jenny Stout, Richard King, Alan Wahren, Britta Genet Vaccines Ther Research BACKGROUND: The use of optimized delivery devices has been shown to enhance the potency of DNA vaccines. However, further optimization of DNA vaccine delivery is needed for this vaccine modality to ultimately be efficacious in humans. METHODS: Herein we evaluated antigen expression and immunogenicity after intradermal delivery of different doses of DNA vaccines by needle or by the Biojector jet-injection device, with or without the addition of electroporation (EP). RESULTS: Neither needle injection augmented by EP nor Biojector alone could induce higher magnitudes of immune responses after immunizations with a high dose of DNA. After division of a defined DNA dose into multiple skin sites, the humoral response was particularly enhanced by Biojector while cellular responses were particularly enhanced by EP. Furthermore, a close correlation between in vivo antigen expression and cell-mediated as well as humoral immune responses was observed. CONCLUSIONS: These results show that two optimized DNA vaccine delivery devices can act together to overcome dose restrictions of plasmid DNA vaccines. BioMed Central 2012-08-08 /pmc/articles/PMC3532290/ /pubmed/22873174 http://dx.doi.org/10.1186/1479-0556-10-5 Text en Copyright ©2012 Hallengard et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Hallengärd, David Bråve, Andreas Isaguliants, Maria Blomberg, Pontus Enger, Jenny Stout, Richard King, Alan Wahren, Britta A combination of intradermal jet-injection and electroporation overcomes in vivo dose restriction of DNA vaccines |
title | A combination of intradermal jet-injection and electroporation overcomes in vivo dose restriction of DNA vaccines |
title_full | A combination of intradermal jet-injection and electroporation overcomes in vivo dose restriction of DNA vaccines |
title_fullStr | A combination of intradermal jet-injection and electroporation overcomes in vivo dose restriction of DNA vaccines |
title_full_unstemmed | A combination of intradermal jet-injection and electroporation overcomes in vivo dose restriction of DNA vaccines |
title_short | A combination of intradermal jet-injection and electroporation overcomes in vivo dose restriction of DNA vaccines |
title_sort | combination of intradermal jet-injection and electroporation overcomes in vivo dose restriction of dna vaccines |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3532290/ https://www.ncbi.nlm.nih.gov/pubmed/22873174 http://dx.doi.org/10.1186/1479-0556-10-5 |
work_keys_str_mv | AT hallengarddavid acombinationofintradermaljetinjectionandelectroporationovercomesinvivodoserestrictionofdnavaccines AT braveandreas acombinationofintradermaljetinjectionandelectroporationovercomesinvivodoserestrictionofdnavaccines AT isaguliantsmaria acombinationofintradermaljetinjectionandelectroporationovercomesinvivodoserestrictionofdnavaccines AT blombergpontus acombinationofintradermaljetinjectionandelectroporationovercomesinvivodoserestrictionofdnavaccines AT engerjenny acombinationofintradermaljetinjectionandelectroporationovercomesinvivodoserestrictionofdnavaccines AT stoutrichard acombinationofintradermaljetinjectionandelectroporationovercomesinvivodoserestrictionofdnavaccines AT kingalan acombinationofintradermaljetinjectionandelectroporationovercomesinvivodoserestrictionofdnavaccines AT wahrenbritta acombinationofintradermaljetinjectionandelectroporationovercomesinvivodoserestrictionofdnavaccines AT hallengarddavid combinationofintradermaljetinjectionandelectroporationovercomesinvivodoserestrictionofdnavaccines AT braveandreas combinationofintradermaljetinjectionandelectroporationovercomesinvivodoserestrictionofdnavaccines AT isaguliantsmaria combinationofintradermaljetinjectionandelectroporationovercomesinvivodoserestrictionofdnavaccines AT blombergpontus combinationofintradermaljetinjectionandelectroporationovercomesinvivodoserestrictionofdnavaccines AT engerjenny combinationofintradermaljetinjectionandelectroporationovercomesinvivodoserestrictionofdnavaccines AT stoutrichard combinationofintradermaljetinjectionandelectroporationovercomesinvivodoserestrictionofdnavaccines AT kingalan combinationofintradermaljetinjectionandelectroporationovercomesinvivodoserestrictionofdnavaccines AT wahrenbritta combinationofintradermaljetinjectionandelectroporationovercomesinvivodoserestrictionofdnavaccines |