Quantitative, Non-Disruptive Monitoring of Transcription in Single Cells with a Broad-Host Range GFP-luxCDABE Dual Reporter System

A dual promoter probe system based on a tandem bi-cistronic GFP-luxCDABE reporter cassette is described and implemented. This system is assembled in two synthetic, modular, broad-host range plasmids based on pBBR1 and RK2 origins of replication, allowing its utilization in an extensive number of gra...

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Detalles Bibliográficos
Autores principales: Benedetti, Ilaria Maria, de Lorenzo, Victor, Silva-Rocha, Rafael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3532404/
https://www.ncbi.nlm.nih.gov/pubmed/23284849
http://dx.doi.org/10.1371/journal.pone.0052000

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3532404/
https://www.ncbi.nlm.nih.gov/pubmed/23284849
http://dx.doi.org/10.1371/journal.pone.0052000

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