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Mass spectrometry analysis of human P2X1 receptors; insight into phosphorylation, modelling and conformational changes

Recombinant FlagHis(6) tagged Human P2X1 receptors expressed in HEK293 cells were purified, digested with trypsin and analysed by mass spectroscopy (96% coverage following de-glycosylation and reduction). The receptor was basally phosphorylated at residues S387, S388 and T389 in the carboxyl terminu...

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Detalles Bibliográficos
Autores principales:	Roberts, Jonathan A, Bottrill, Andrew R, Mistry, Sharad, Evans, Richard J
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Blackwell Publishing Ltd 2012
Materias:	Original Articles
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3532615/ https://www.ncbi.nlm.nih.gov/pubmed/22971236 http://dx.doi.org/10.1111/jnc.12012

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