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A Sugar Gustatory Receptor Identified from the Foregut of Cotton Bollworm Helicoverpa armigera

Helicoverpa armigera (Hübner) is one of the most polyphagous and cosmopolitan pest species, the larvae of which feed on numerous important crops. The gustatory system is critical in guiding insect feeding behavior. Here, we identified a gustatory receptor from H. armigera, HaGR9, which shows high le...

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Autores principales: Xu, Wei, Zhang, Hui-Jie, Anderson, Alisha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3532720/
https://www.ncbi.nlm.nih.gov/pubmed/23224441
http://dx.doi.org/10.1007/s10886-012-0221-8
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author Xu, Wei
Zhang, Hui-Jie
Anderson, Alisha
author_facet Xu, Wei
Zhang, Hui-Jie
Anderson, Alisha
author_sort Xu, Wei
collection PubMed
description Helicoverpa armigera (Hübner) is one of the most polyphagous and cosmopolitan pest species, the larvae of which feed on numerous important crops. The gustatory system is critical in guiding insect feeding behavior. Here, we identified a gustatory receptor from H. armigera, HaGR9, which shows high levels of identity to DmGR43a from Drosophila melanogaster and BmGR9 from Bombyx mori. Reverse transcriptase PCR (RT-PCR) revealed HaGR9 is highly expressed in larval foregut, with little or no expression in other chemosensory tissues. Membrane topology studies indicated that, like two previously studied B. mori GRs, BmGR8 and BmGR53, HaGR9 has an inverted topology relative to G protein-coupled receptors (GPCRs), an intracellular N-terminus and an extracellular C-terminus. Calcium imaging studies confirmed HaGR9 is a sugar receptor showing dose-dependent responses to D-galactose, D-maltose, and D-fructose. This highly-expressed foregut-specific gustatory receptor may contribute to the regulation of larval feeding behavior.
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spelling pubmed-35327202013-01-04 A Sugar Gustatory Receptor Identified from the Foregut of Cotton Bollworm Helicoverpa armigera Xu, Wei Zhang, Hui-Jie Anderson, Alisha J Chem Ecol Article Helicoverpa armigera (Hübner) is one of the most polyphagous and cosmopolitan pest species, the larvae of which feed on numerous important crops. The gustatory system is critical in guiding insect feeding behavior. Here, we identified a gustatory receptor from H. armigera, HaGR9, which shows high levels of identity to DmGR43a from Drosophila melanogaster and BmGR9 from Bombyx mori. Reverse transcriptase PCR (RT-PCR) revealed HaGR9 is highly expressed in larval foregut, with little or no expression in other chemosensory tissues. Membrane topology studies indicated that, like two previously studied B. mori GRs, BmGR8 and BmGR53, HaGR9 has an inverted topology relative to G protein-coupled receptors (GPCRs), an intracellular N-terminus and an extracellular C-terminus. Calcium imaging studies confirmed HaGR9 is a sugar receptor showing dose-dependent responses to D-galactose, D-maltose, and D-fructose. This highly-expressed foregut-specific gustatory receptor may contribute to the regulation of larval feeding behavior. Springer-Verlag 2012-12-06 2012 /pmc/articles/PMC3532720/ /pubmed/23224441 http://dx.doi.org/10.1007/s10886-012-0221-8 Text en © The Author(s) 2012 https://creativecommons.org/licenses/by/2.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Article
Xu, Wei
Zhang, Hui-Jie
Anderson, Alisha
A Sugar Gustatory Receptor Identified from the Foregut of Cotton Bollworm Helicoverpa armigera
title A Sugar Gustatory Receptor Identified from the Foregut of Cotton Bollworm Helicoverpa armigera
title_full A Sugar Gustatory Receptor Identified from the Foregut of Cotton Bollworm Helicoverpa armigera
title_fullStr A Sugar Gustatory Receptor Identified from the Foregut of Cotton Bollworm Helicoverpa armigera
title_full_unstemmed A Sugar Gustatory Receptor Identified from the Foregut of Cotton Bollworm Helicoverpa armigera
title_short A Sugar Gustatory Receptor Identified from the Foregut of Cotton Bollworm Helicoverpa armigera
title_sort sugar gustatory receptor identified from the foregut of cotton bollworm helicoverpa armigera
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3532720/
https://www.ncbi.nlm.nih.gov/pubmed/23224441
http://dx.doi.org/10.1007/s10886-012-0221-8
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