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Mansonella ozzardi corneal lesions in the Amazon: a cross-sectional study

OBJECTIVES: To characterise and confirm the presence of Mansonella ozzardi microfilariae in the cornea by biomicroscopy and corneal confocal microscopy. DESIGN: Cross-sectional study. SETTINGS: Clinical practice study in patients from rural communities in Coari city on the Solimões river, Amazonas s...

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Autores principales: Vianna, Lucas Monferrari Monteiro, Martins, Marilaine, Cohen, Marcos Jacob, Cohen, Jacob Moyses, Belfort, Rubens
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BMJ Publishing Group 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3533123/
https://www.ncbi.nlm.nih.gov/pubmed/23187969
http://dx.doi.org/10.1136/bmjopen-2012-001266
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author Vianna, Lucas Monferrari Monteiro
Martins, Marilaine
Cohen, Marcos Jacob
Cohen, Jacob Moyses
Belfort, Rubens
author_facet Vianna, Lucas Monferrari Monteiro
Martins, Marilaine
Cohen, Marcos Jacob
Cohen, Jacob Moyses
Belfort, Rubens
author_sort Vianna, Lucas Monferrari Monteiro
collection PubMed
description OBJECTIVES: To characterise and confirm the presence of Mansonella ozzardi microfilariae in the cornea by biomicroscopy and corneal confocal microscopy. DESIGN: Cross-sectional study. SETTINGS: Clinical practice study in patients from rural communities in Coari city on the Solimões river, Amazonas state, Brazil. PARTICIPANTS: The eyes of 212 consecutive volunteer patients were examined using a flash light and their blood checked for the presence of microfilariae by an expert microscopist. Patients with suspicious corneal lesions (characterised as nummular keratitis) were submitted to biomicroscopy, fundoscopy and corneal confocal microscopy evaluation (CCME). In two patients, a biopsy of the limbal conjunctiva adjacent to the nummular keratitis was carried out and blood collected from the surgical wound for microfilariae investigation by thick blood film examination. PRIMARY AND SECONDARY OUTCOME MEASURES: Positive correlation between corneal biomicroscopic and confocal lesions and M ozzardi microfilaremia. RESULTS: Of the 212 patients, 56 (26.4%) were positive for microfilaremia. 22 patients with nummular keratitis identified under flash light examination underwent biomicroscopy and CCME. Corneal lesions were positively correlated to microfilaremia (p=0.0001). At biomicroscopy, lesions were classified as quiescent or active. At CCME, lesions were categorised as circular or filiform. The associations between corneal lesions, CCME findings and microfilaremia are shown. CONCLUSIONS: We describe M ozzardi microfilariae in the cornea and the associated eye pathology. Further studies using ocular tissue PCR and other imaging techniques would be helpful.
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spelling pubmed-35331232013-01-04 Mansonella ozzardi corneal lesions in the Amazon: a cross-sectional study Vianna, Lucas Monferrari Monteiro Martins, Marilaine Cohen, Marcos Jacob Cohen, Jacob Moyses Belfort, Rubens BMJ Open Addiction OBJECTIVES: To characterise and confirm the presence of Mansonella ozzardi microfilariae in the cornea by biomicroscopy and corneal confocal microscopy. DESIGN: Cross-sectional study. SETTINGS: Clinical practice study in patients from rural communities in Coari city on the Solimões river, Amazonas state, Brazil. PARTICIPANTS: The eyes of 212 consecutive volunteer patients were examined using a flash light and their blood checked for the presence of microfilariae by an expert microscopist. Patients with suspicious corneal lesions (characterised as nummular keratitis) were submitted to biomicroscopy, fundoscopy and corneal confocal microscopy evaluation (CCME). In two patients, a biopsy of the limbal conjunctiva adjacent to the nummular keratitis was carried out and blood collected from the surgical wound for microfilariae investigation by thick blood film examination. PRIMARY AND SECONDARY OUTCOME MEASURES: Positive correlation between corneal biomicroscopic and confocal lesions and M ozzardi microfilaremia. RESULTS: Of the 212 patients, 56 (26.4%) were positive for microfilaremia. 22 patients with nummular keratitis identified under flash light examination underwent biomicroscopy and CCME. Corneal lesions were positively correlated to microfilaremia (p=0.0001). At biomicroscopy, lesions were classified as quiescent or active. At CCME, lesions were categorised as circular or filiform. The associations between corneal lesions, CCME findings and microfilaremia are shown. CONCLUSIONS: We describe M ozzardi microfilariae in the cornea and the associated eye pathology. Further studies using ocular tissue PCR and other imaging techniques would be helpful. BMJ Publishing Group 2012-11-26 /pmc/articles/PMC3533123/ /pubmed/23187969 http://dx.doi.org/10.1136/bmjopen-2012-001266 Text en Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions This is an open-access article distributed under the terms of the Creative Commons Attribution Non-commercial License, which permits use, distribution, and reproduction in any medium, provided the original work is properly cited, the use is non commercial and is otherwise in compliance with the license. See: http://creativecommons.org/licenses/by-nc/2.0/ and http://creativecommons.org/licenses/by-nc/2.0/legalcode.
spellingShingle Addiction
Vianna, Lucas Monferrari Monteiro
Martins, Marilaine
Cohen, Marcos Jacob
Cohen, Jacob Moyses
Belfort, Rubens
Mansonella ozzardi corneal lesions in the Amazon: a cross-sectional study
title Mansonella ozzardi corneal lesions in the Amazon: a cross-sectional study
title_full Mansonella ozzardi corneal lesions in the Amazon: a cross-sectional study
title_fullStr Mansonella ozzardi corneal lesions in the Amazon: a cross-sectional study
title_full_unstemmed Mansonella ozzardi corneal lesions in the Amazon: a cross-sectional study
title_short Mansonella ozzardi corneal lesions in the Amazon: a cross-sectional study
title_sort mansonella ozzardi corneal lesions in the amazon: a cross-sectional study
topic Addiction
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3533123/
https://www.ncbi.nlm.nih.gov/pubmed/23187969
http://dx.doi.org/10.1136/bmjopen-2012-001266
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