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Limitations and possibilities of low cell number ChIP-seq

BACKGROUND: Chromatin immunoprecipitation coupled with high-throughput DNA sequencing (ChIP-seq) offers high resolution, genome-wide analysis of DNA-protein interactions. However, current standard methods require abundant starting material in the range of 1–20 million cells per immunoprecipitation,...

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Detalles Bibliográficos
Autores principales:	Gilfillan, Gregor D, Hughes, Timothy, Sheng, Ying, Hjorthaug, Hanne S, Straub, Tobias, Gervin, Kristina, Harris, Jennifer R, Undlien, Dag E, Lyle, Robert
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2012
Materias:	Methodology Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3533509/ https://www.ncbi.nlm.nih.gov/pubmed/23171294 http://dx.doi.org/10.1186/1471-2164-13-645

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