Cargando…

The role of nitric oxide on rosuvastatin-mediated S-nitrosylation and translational proteomes in human umbilical vein endothelial cells

BACKGROUND: The pleiotropic effects of 3-Hydroxy-3-methylglutaryl coenzyme A reductase inhibitors (statins), which are independent from their cholesterol-lowering action, have been widely recognized in various biological systems. Statins can affect endothelial homeostasis, which is partly modulated...

Descripción completa

Detalles Bibliográficos
Autores principales: Huang, Bin, Li, Fu An, Wu, Chien Hsing, Wang, Danny Ling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3533754/
https://www.ncbi.nlm.nih.gov/pubmed/22799578
http://dx.doi.org/10.1186/1477-5956-10-43
_version_ 1782254449083809792
author Huang, Bin
Li, Fu An
Wu, Chien Hsing
Wang, Danny Ling
author_facet Huang, Bin
Li, Fu An
Wu, Chien Hsing
Wang, Danny Ling
author_sort Huang, Bin
collection PubMed
description BACKGROUND: The pleiotropic effects of 3-Hydroxy-3-methylglutaryl coenzyme A reductase inhibitors (statins), which are independent from their cholesterol-lowering action, have been widely recognized in various biological systems. Statins can affect endothelial homeostasis, which is partly modulated by the production of nitric oxide (NO). However, it is unclear how statin/NO-mediated posttranslational S-nitrosylation of endothelial proteins and changes in translational profiles may benefit endothelial integrity. Therefore, it is important to understand the statin/NO-mediated S-nitrosylation in endothelial cells. RESULTS: Rosuvastatin treatment of human umbilical vein endothelial cells (ECs) enhanced the enzymatic activity of endothelial nitric oxide synthase (eNOS) and the expression of 78 S-nitrosoproteins. Among these S-nitrosoproteins, we identified 17 proteins, including protein disulfide bond isomerase, phospholipase C, transaldolase and heat shock proteins. Furthermore, a hydrophobic Cys66 was determined as the S-nitrosylation site of the mitochondrial HSP70. In addition to the statin-modulated posttranslational S-nitrosylation, changes in the NO-mediated translational proteome were also observed. Seventeen major proteins were significantly upregulated after rosuvastatin treatment. However, 12 of these proteins were downregulated after pretreating ECs with an eNOS inhibitor (L-NAME), which indicated that their expression was modulated by NO. CONCLUSIONS: ECs treated with rosuvastatin increase eNOS activation. The increased NO production is involved in modulating S-nitrosylation and translation of proteins. We provide further evidence of the pleiotropic effect of rosuvastatin on endothelial physiology.
format Online
Article
Text
id pubmed-3533754
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-35337542013-01-03 The role of nitric oxide on rosuvastatin-mediated S-nitrosylation and translational proteomes in human umbilical vein endothelial cells Huang, Bin Li, Fu An Wu, Chien Hsing Wang, Danny Ling Proteome Sci Research BACKGROUND: The pleiotropic effects of 3-Hydroxy-3-methylglutaryl coenzyme A reductase inhibitors (statins), which are independent from their cholesterol-lowering action, have been widely recognized in various biological systems. Statins can affect endothelial homeostasis, which is partly modulated by the production of nitric oxide (NO). However, it is unclear how statin/NO-mediated posttranslational S-nitrosylation of endothelial proteins and changes in translational profiles may benefit endothelial integrity. Therefore, it is important to understand the statin/NO-mediated S-nitrosylation in endothelial cells. RESULTS: Rosuvastatin treatment of human umbilical vein endothelial cells (ECs) enhanced the enzymatic activity of endothelial nitric oxide synthase (eNOS) and the expression of 78 S-nitrosoproteins. Among these S-nitrosoproteins, we identified 17 proteins, including protein disulfide bond isomerase, phospholipase C, transaldolase and heat shock proteins. Furthermore, a hydrophobic Cys66 was determined as the S-nitrosylation site of the mitochondrial HSP70. In addition to the statin-modulated posttranslational S-nitrosylation, changes in the NO-mediated translational proteome were also observed. Seventeen major proteins were significantly upregulated after rosuvastatin treatment. However, 12 of these proteins were downregulated after pretreating ECs with an eNOS inhibitor (L-NAME), which indicated that their expression was modulated by NO. CONCLUSIONS: ECs treated with rosuvastatin increase eNOS activation. The increased NO production is involved in modulating S-nitrosylation and translation of proteins. We provide further evidence of the pleiotropic effect of rosuvastatin on endothelial physiology. BioMed Central 2012-07-16 /pmc/articles/PMC3533754/ /pubmed/22799578 http://dx.doi.org/10.1186/1477-5956-10-43 Text en Copyright © 2012 Huang et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Huang, Bin
Li, Fu An
Wu, Chien Hsing
Wang, Danny Ling
The role of nitric oxide on rosuvastatin-mediated S-nitrosylation and translational proteomes in human umbilical vein endothelial cells
title The role of nitric oxide on rosuvastatin-mediated S-nitrosylation and translational proteomes in human umbilical vein endothelial cells
title_full The role of nitric oxide on rosuvastatin-mediated S-nitrosylation and translational proteomes in human umbilical vein endothelial cells
title_fullStr The role of nitric oxide on rosuvastatin-mediated S-nitrosylation and translational proteomes in human umbilical vein endothelial cells
title_full_unstemmed The role of nitric oxide on rosuvastatin-mediated S-nitrosylation and translational proteomes in human umbilical vein endothelial cells
title_short The role of nitric oxide on rosuvastatin-mediated S-nitrosylation and translational proteomes in human umbilical vein endothelial cells
title_sort role of nitric oxide on rosuvastatin-mediated s-nitrosylation and translational proteomes in human umbilical vein endothelial cells
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3533754/
https://www.ncbi.nlm.nih.gov/pubmed/22799578
http://dx.doi.org/10.1186/1477-5956-10-43
work_keys_str_mv AT huangbin theroleofnitricoxideonrosuvastatinmediatedsnitrosylationandtranslationalproteomesinhumanumbilicalveinendothelialcells
AT lifuan theroleofnitricoxideonrosuvastatinmediatedsnitrosylationandtranslationalproteomesinhumanumbilicalveinendothelialcells
AT wuchienhsing theroleofnitricoxideonrosuvastatinmediatedsnitrosylationandtranslationalproteomesinhumanumbilicalveinendothelialcells
AT wangdannyling theroleofnitricoxideonrosuvastatinmediatedsnitrosylationandtranslationalproteomesinhumanumbilicalveinendothelialcells
AT huangbin roleofnitricoxideonrosuvastatinmediatedsnitrosylationandtranslationalproteomesinhumanumbilicalveinendothelialcells
AT lifuan roleofnitricoxideonrosuvastatinmediatedsnitrosylationandtranslationalproteomesinhumanumbilicalveinendothelialcells
AT wuchienhsing roleofnitricoxideonrosuvastatinmediatedsnitrosylationandtranslationalproteomesinhumanumbilicalveinendothelialcells
AT wangdannyling roleofnitricoxideonrosuvastatinmediatedsnitrosylationandtranslationalproteomesinhumanumbilicalveinendothelialcells