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The Snf1-related protein kinases SnRK2.4 and SnRK2.10 are involved in maintenance of root system architecture during salt stress

The sucrose non-fermenting-1-related protein kinase 2 (SnRK2) family represents a unique family of plant-specific protein kinases implicated in cellular signalling in response to osmotic stress. In our studies, we observed that two class 1 SnRK2 kinases, SnRK2.4 and SnRK2.10, are rapidly and transie...

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Autores principales: McLoughlin, Fionn, Galvan-Ampudia, Carlos S, Julkowska, Magdalena M, Caarls, Lotte, van der Does, Dieuwertje, Laurière, Christiane, Munnik, Teun, Haring, Michel A, Testerink, Christa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3533798/
https://www.ncbi.nlm.nih.gov/pubmed/22738204
http://dx.doi.org/10.1111/j.1365-313X.2012.05089.x
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author McLoughlin, Fionn
Galvan-Ampudia, Carlos S
Julkowska, Magdalena M
Caarls, Lotte
van der Does, Dieuwertje
Laurière, Christiane
Munnik, Teun
Haring, Michel A
Testerink, Christa
author_facet McLoughlin, Fionn
Galvan-Ampudia, Carlos S
Julkowska, Magdalena M
Caarls, Lotte
van der Does, Dieuwertje
Laurière, Christiane
Munnik, Teun
Haring, Michel A
Testerink, Christa
author_sort McLoughlin, Fionn
collection PubMed
description The sucrose non-fermenting-1-related protein kinase 2 (SnRK2) family represents a unique family of plant-specific protein kinases implicated in cellular signalling in response to osmotic stress. In our studies, we observed that two class 1 SnRK2 kinases, SnRK2.4 and SnRK2.10, are rapidly and transiently activated in Arabidopsis roots after exposure to salt. Under saline conditions, snrk2.4 knockout mutants had a reduced primary root length, while snrk2.10 mutants exhibited a reduction in the number of lateral roots. The reduced lateral root density was found to be a combinatory effect of a decrease in the number of lateral root primordia and an increase in the number of arrested lateral root primordia. The phenotypes were in agreement with the observed expression patterns of genomic yellow fluorescent protein (YFP) fusions of SnRK2.10 and -2.4, under control of their native promoter sequences. SnRK2.10 was found to be expressed in the vascular tissue at the base of a developing lateral root, whereas SnRK2.4 was expressed throughout the root, with higher expression in the vascular system. Salt stress triggered a rapid re-localization of SnRK2.4–YFP from the cytosol to punctate structures in root epidermal cells. Differential centrifugation experiments of isolated Arabidopsis root proteins confirmed recruitment of endogenous SnRK2.4/2.10 to membranes upon exposure to salt, supporting their observed binding affinity for the phospholipid phosphatidic acid. Together, our results reveal a role for SnRK2.4 and -2.10 in root growth and architecture in saline conditions.
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spelling pubmed-35337982013-01-08 The Snf1-related protein kinases SnRK2.4 and SnRK2.10 are involved in maintenance of root system architecture during salt stress McLoughlin, Fionn Galvan-Ampudia, Carlos S Julkowska, Magdalena M Caarls, Lotte van der Does, Dieuwertje Laurière, Christiane Munnik, Teun Haring, Michel A Testerink, Christa Plant J Original Articles The sucrose non-fermenting-1-related protein kinase 2 (SnRK2) family represents a unique family of plant-specific protein kinases implicated in cellular signalling in response to osmotic stress. In our studies, we observed that two class 1 SnRK2 kinases, SnRK2.4 and SnRK2.10, are rapidly and transiently activated in Arabidopsis roots after exposure to salt. Under saline conditions, snrk2.4 knockout mutants had a reduced primary root length, while snrk2.10 mutants exhibited a reduction in the number of lateral roots. The reduced lateral root density was found to be a combinatory effect of a decrease in the number of lateral root primordia and an increase in the number of arrested lateral root primordia. The phenotypes were in agreement with the observed expression patterns of genomic yellow fluorescent protein (YFP) fusions of SnRK2.10 and -2.4, under control of their native promoter sequences. SnRK2.10 was found to be expressed in the vascular tissue at the base of a developing lateral root, whereas SnRK2.4 was expressed throughout the root, with higher expression in the vascular system. Salt stress triggered a rapid re-localization of SnRK2.4–YFP from the cytosol to punctate structures in root epidermal cells. Differential centrifugation experiments of isolated Arabidopsis root proteins confirmed recruitment of endogenous SnRK2.4/2.10 to membranes upon exposure to salt, supporting their observed binding affinity for the phospholipid phosphatidic acid. Together, our results reveal a role for SnRK2.4 and -2.10 in root growth and architecture in saline conditions. Blackwell Publishing Ltd 2012-11 2012-08-20 /pmc/articles/PMC3533798/ /pubmed/22738204 http://dx.doi.org/10.1111/j.1365-313X.2012.05089.x Text en © 2012 The Authors. The Plant Journal © 2012 Blackwell Publishing Ltd http://creativecommons.org/licenses/by/2.5/ Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.
spellingShingle Original Articles
McLoughlin, Fionn
Galvan-Ampudia, Carlos S
Julkowska, Magdalena M
Caarls, Lotte
van der Does, Dieuwertje
Laurière, Christiane
Munnik, Teun
Haring, Michel A
Testerink, Christa
The Snf1-related protein kinases SnRK2.4 and SnRK2.10 are involved in maintenance of root system architecture during salt stress
title The Snf1-related protein kinases SnRK2.4 and SnRK2.10 are involved in maintenance of root system architecture during salt stress
title_full The Snf1-related protein kinases SnRK2.4 and SnRK2.10 are involved in maintenance of root system architecture during salt stress
title_fullStr The Snf1-related protein kinases SnRK2.4 and SnRK2.10 are involved in maintenance of root system architecture during salt stress
title_full_unstemmed The Snf1-related protein kinases SnRK2.4 and SnRK2.10 are involved in maintenance of root system architecture during salt stress
title_short The Snf1-related protein kinases SnRK2.4 and SnRK2.10 are involved in maintenance of root system architecture during salt stress
title_sort snf1-related protein kinases snrk2.4 and snrk2.10 are involved in maintenance of root system architecture during salt stress
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3533798/
https://www.ncbi.nlm.nih.gov/pubmed/22738204
http://dx.doi.org/10.1111/j.1365-313X.2012.05089.x
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