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A Novel Immunofluorescent Computed Tomography (ICT) Method to Localise and Quantify Multiple Antigens in Large Tissue Volumes at High Resolution

Current immunofluorescence protocols are limited as they do not provide reliable antibody staining within large tissue volumes (mm(3)) and cannot localise and quantify multiple antigens or cell populations in the same tissue at high resolution. To address this limitation, we have developed an approa...

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Detalles Bibliográficos
Autores principales: Parfitt, Geraint J., Xie, Yilu, Reid, Korey M., Dervillez, Xavier, Brown, Donald J., Jester, James V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3534019/
https://www.ncbi.nlm.nih.gov/pubmed/23300899
http://dx.doi.org/10.1371/journal.pone.0053245