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A Novel Immunofluorescent Computed Tomography (ICT) Method to Localise and Quantify Multiple Antigens in Large Tissue Volumes at High Resolution
Current immunofluorescence protocols are limited as they do not provide reliable antibody staining within large tissue volumes (mm(3)) and cannot localise and quantify multiple antigens or cell populations in the same tissue at high resolution. To address this limitation, we have developed an approa...
Autores principales: | Parfitt, Geraint J., Xie, Yilu, Reid, Korey M., Dervillez, Xavier, Brown, Donald J., Jester, James V. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3534019/ https://www.ncbi.nlm.nih.gov/pubmed/23300899 http://dx.doi.org/10.1371/journal.pone.0053245 |
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