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Genistein Suppresses LPS-Induced Inflammatory Response through Inhibiting NF-κB following AMP Kinase Activation in RAW 264.7 Macrophages

Genistein, the major isoflavone in soybean, was recently reported to exert beneficial effects in metabolic disorders and inflammatory diseases. In the present study, we investigated the effects and mechanisms of a dietary concentration of genistein on the inflammatory response in lipopolysaccharide...

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Autores principales: Ji, Guiyuan, Zhang, Yupei, Yang, Qinhe, Cheng, Shaobin, Hao, Jing, Zhao, Xihong, Jiang, Zhuoqin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3534028/
https://www.ncbi.nlm.nih.gov/pubmed/23300870
http://dx.doi.org/10.1371/journal.pone.0053101
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author Ji, Guiyuan
Zhang, Yupei
Yang, Qinhe
Cheng, Shaobin
Hao, Jing
Zhao, Xihong
Jiang, Zhuoqin
author_facet Ji, Guiyuan
Zhang, Yupei
Yang, Qinhe
Cheng, Shaobin
Hao, Jing
Zhao, Xihong
Jiang, Zhuoqin
author_sort Ji, Guiyuan
collection PubMed
description Genistein, the major isoflavone in soybean, was recently reported to exert beneficial effects in metabolic disorders and inflammatory diseases. In the present study, we investigated the effects and mechanisms of a dietary concentration of genistein on the inflammatory response in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages. Our results demonstrated that genistein effectively inhibited the LPS-induced overproduction of tumor necrosis factor-alpha (TNF-α) and interleukin 6 (IL-6), as well as LPS-induced nuclear factor kappa B (NF-κB) activation. In addition, the data also showed that genistein prevented LPS-induced decrease in adenosine monophosphate-activated protein kinase (AMPK) phosphorylation. These effects were obviously attenuated by an AMPK inhibitor. Taken together, our results suggest that the dietary concentration of genistein is able to attenuate inflammatory responses via inhibition of NF-κB activation following AMPK stimulation. The data provide direct evidence for the potential application of low concentrations of genistein in the prevention and treatment of inflammatory diseases.
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spelling pubmed-35340282013-01-08 Genistein Suppresses LPS-Induced Inflammatory Response through Inhibiting NF-κB following AMP Kinase Activation in RAW 264.7 Macrophages Ji, Guiyuan Zhang, Yupei Yang, Qinhe Cheng, Shaobin Hao, Jing Zhao, Xihong Jiang, Zhuoqin PLoS One Research Article Genistein, the major isoflavone in soybean, was recently reported to exert beneficial effects in metabolic disorders and inflammatory diseases. In the present study, we investigated the effects and mechanisms of a dietary concentration of genistein on the inflammatory response in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages. Our results demonstrated that genistein effectively inhibited the LPS-induced overproduction of tumor necrosis factor-alpha (TNF-α) and interleukin 6 (IL-6), as well as LPS-induced nuclear factor kappa B (NF-κB) activation. In addition, the data also showed that genistein prevented LPS-induced decrease in adenosine monophosphate-activated protein kinase (AMPK) phosphorylation. These effects were obviously attenuated by an AMPK inhibitor. Taken together, our results suggest that the dietary concentration of genistein is able to attenuate inflammatory responses via inhibition of NF-κB activation following AMPK stimulation. The data provide direct evidence for the potential application of low concentrations of genistein in the prevention and treatment of inflammatory diseases. Public Library of Science 2012-12-31 /pmc/articles/PMC3534028/ /pubmed/23300870 http://dx.doi.org/10.1371/journal.pone.0053101 Text en © 2012 Ji et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Ji, Guiyuan
Zhang, Yupei
Yang, Qinhe
Cheng, Shaobin
Hao, Jing
Zhao, Xihong
Jiang, Zhuoqin
Genistein Suppresses LPS-Induced Inflammatory Response through Inhibiting NF-κB following AMP Kinase Activation in RAW 264.7 Macrophages
title Genistein Suppresses LPS-Induced Inflammatory Response through Inhibiting NF-κB following AMP Kinase Activation in RAW 264.7 Macrophages
title_full Genistein Suppresses LPS-Induced Inflammatory Response through Inhibiting NF-κB following AMP Kinase Activation in RAW 264.7 Macrophages
title_fullStr Genistein Suppresses LPS-Induced Inflammatory Response through Inhibiting NF-κB following AMP Kinase Activation in RAW 264.7 Macrophages
title_full_unstemmed Genistein Suppresses LPS-Induced Inflammatory Response through Inhibiting NF-κB following AMP Kinase Activation in RAW 264.7 Macrophages
title_short Genistein Suppresses LPS-Induced Inflammatory Response through Inhibiting NF-κB following AMP Kinase Activation in RAW 264.7 Macrophages
title_sort genistein suppresses lps-induced inflammatory response through inhibiting nf-κb following amp kinase activation in raw 264.7 macrophages
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3534028/
https://www.ncbi.nlm.nih.gov/pubmed/23300870
http://dx.doi.org/10.1371/journal.pone.0053101
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