rsEGFP2 enables fast RESOLFT nanoscopy of living cells

The super-resolution microscopy called RESOLFT relying on fluorophore switching between longlived states, stands out by its coordinate-targeted sequential sample interrogation using low light levels. While RESOLFT has been shown to discern nanostructures in living cells, the reversibly photoswitchab...

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Detalles Bibliográficos
Autores principales: Grotjohann, Tim, Testa, Ilaria, Reuss, Matthias, Brakemann, Tanja, Eggeling, Christian, Hell, Stefan W, Jakobs, Stefan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2012
Materias:
Biophysics and Structural Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3534202/
https://www.ncbi.nlm.nih.gov/pubmed/23330067
http://dx.doi.org/10.7554/eLife.00248
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author Grotjohann, Tim
Testa, Ilaria
Reuss, Matthias
Brakemann, Tanja
Eggeling, Christian
Hell, Stefan W
Jakobs, Stefan
author_facet Grotjohann, Tim
Testa, Ilaria
Reuss, Matthias
Brakemann, Tanja
Eggeling, Christian
Hell, Stefan W
Jakobs, Stefan
author_sort Grotjohann, Tim
collection PubMed
description The super-resolution microscopy called RESOLFT relying on fluorophore switching between longlived states, stands out by its coordinate-targeted sequential sample interrogation using low light levels. While RESOLFT has been shown to discern nanostructures in living cells, the reversibly photoswitchable green fluorescent protein (rsEGFP) employed in these experiments was switched rather slowly and recording lasted tens of minutes. We now report on the generation of rsEGFP2 providing faster switching and the use of this protein to demonstrate 25–250 times faster recordings. DOI: http://dx.doi.org/10.7554/eLife.00248.001
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spelling pubmed-35342022013-01-17 rsEGFP2 enables fast RESOLFT nanoscopy of living cells Grotjohann, Tim Testa, Ilaria Reuss, Matthias Brakemann, Tanja Eggeling, Christian Hell, Stefan W Jakobs, Stefan eLife Biophysics and Structural Biology The super-resolution microscopy called RESOLFT relying on fluorophore switching between longlived states, stands out by its coordinate-targeted sequential sample interrogation using low light levels. While RESOLFT has been shown to discern nanostructures in living cells, the reversibly photoswitchable green fluorescent protein (rsEGFP) employed in these experiments was switched rather slowly and recording lasted tens of minutes. We now report on the generation of rsEGFP2 providing faster switching and the use of this protein to demonstrate 25–250 times faster recordings. DOI: http://dx.doi.org/10.7554/eLife.00248.001 eLife Sciences Publications, Ltd 2012-12-31 /pmc/articles/PMC3534202/ /pubmed/23330067 http://dx.doi.org/10.7554/eLife.00248 Text en Copyright © 2012, Grotjohann et al http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Biophysics and Structural Biology
Grotjohann, Tim
Testa, Ilaria
Reuss, Matthias
Brakemann, Tanja
Eggeling, Christian
Hell, Stefan W
Jakobs, Stefan
rsEGFP2 enables fast RESOLFT nanoscopy of living cells
title rsEGFP2 enables fast RESOLFT nanoscopy of living cells
title_full rsEGFP2 enables fast RESOLFT nanoscopy of living cells
title_fullStr rsEGFP2 enables fast RESOLFT nanoscopy of living cells
title_full_unstemmed rsEGFP2 enables fast RESOLFT nanoscopy of living cells
title_short rsEGFP2 enables fast RESOLFT nanoscopy of living cells
title_sort rsegfp2 enables fast resolft nanoscopy of living cells
topic Biophysics and Structural Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3534202/
https://www.ncbi.nlm.nih.gov/pubmed/23330067
http://dx.doi.org/10.7554/eLife.00248
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