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In vitro stemness characterization of radio-resistant clones isolated from a medulloblastoma cell line ONS-76

One-third of patients with medulloblastoma die due to recurrence after various treatments including radiotherapy. Although it has been postulated that cancer stem-like cells are radio-resistant and play an important role in tumor recurrence, the “stemness” of medulloblastoma cells surviving irradiat...

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Autores principales: Sun, Lue, Moritake, Takashi, Zheng, Yun-Wen, Suzuki, Kenshi, Gerelchuluun, Ariungerel, Hong, Zhengshan, Zenkoh, Junko, Taniguchi, Hideki, Tsuboi, Koji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3534279/
https://www.ncbi.nlm.nih.gov/pubmed/22951319
http://dx.doi.org/10.1093/jrr/rrs078
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author Sun, Lue
Moritake, Takashi
Zheng, Yun-Wen
Suzuki, Kenshi
Gerelchuluun, Ariungerel
Hong, Zhengshan
Zenkoh, Junko
Taniguchi, Hideki
Tsuboi, Koji
author_facet Sun, Lue
Moritake, Takashi
Zheng, Yun-Wen
Suzuki, Kenshi
Gerelchuluun, Ariungerel
Hong, Zhengshan
Zenkoh, Junko
Taniguchi, Hideki
Tsuboi, Koji
author_sort Sun, Lue
collection PubMed
description One-third of patients with medulloblastoma die due to recurrence after various treatments including radiotherapy. Although it has been postulated that cancer stem-like cells are radio-resistant and play an important role in tumor recurrence, the “stemness” of medulloblastoma cells surviving irradiation has not yet been elucidated. Using a medulloblastoma cell line ONS-76, cells that survived gamma irradiation were investigated on their “stemness” in vitro. From 10 500 cells, 20 radio-resistant clones were selected after gamma ray irradiation (5 Gy × two fractions) using the replica micro-well technique. These 20 resistant clones were screened for CD133 positivity by flow cytometry followed by side population assay, tumor sphere formation assay and clonogenic survival assay. Results revealed CD133 fractions were significantly elevated in three clones, which also exhibited significantly increased levels of tumor sphere formation ability and side population fraction. Clonogenic survival assay demonstrated that their radio-resistance was significantly higher than the parental ONS-76. This may support the hypothesis that a small number of cancer stem-like cells (CSCs) are the main culprits in local recurrence after radiotherapy, and disruption of the resistance mechanism of these CSCs is a critical future issue in improving the outcome of patients with medulloblastoma.
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spelling pubmed-35342792013-01-03 In vitro stemness characterization of radio-resistant clones isolated from a medulloblastoma cell line ONS-76 Sun, Lue Moritake, Takashi Zheng, Yun-Wen Suzuki, Kenshi Gerelchuluun, Ariungerel Hong, Zhengshan Zenkoh, Junko Taniguchi, Hideki Tsuboi, Koji J Radiat Res Biology One-third of patients with medulloblastoma die due to recurrence after various treatments including radiotherapy. Although it has been postulated that cancer stem-like cells are radio-resistant and play an important role in tumor recurrence, the “stemness” of medulloblastoma cells surviving irradiation has not yet been elucidated. Using a medulloblastoma cell line ONS-76, cells that survived gamma irradiation were investigated on their “stemness” in vitro. From 10 500 cells, 20 radio-resistant clones were selected after gamma ray irradiation (5 Gy × two fractions) using the replica micro-well technique. These 20 resistant clones were screened for CD133 positivity by flow cytometry followed by side population assay, tumor sphere formation assay and clonogenic survival assay. Results revealed CD133 fractions were significantly elevated in three clones, which also exhibited significantly increased levels of tumor sphere formation ability and side population fraction. Clonogenic survival assay demonstrated that their radio-resistance was significantly higher than the parental ONS-76. This may support the hypothesis that a small number of cancer stem-like cells (CSCs) are the main culprits in local recurrence after radiotherapy, and disruption of the resistance mechanism of these CSCs is a critical future issue in improving the outcome of patients with medulloblastoma. Oxford University Press 2013-01 2012-09-05 /pmc/articles/PMC3534279/ /pubmed/22951319 http://dx.doi.org/10.1093/jrr/rrs078 Text en © The Author 2012. Published by Oxford University Press on behalf of The Japan Radiation Research Society and Japanese Society for Therapeutic Radiology and Oncology. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Biology
Sun, Lue
Moritake, Takashi
Zheng, Yun-Wen
Suzuki, Kenshi
Gerelchuluun, Ariungerel
Hong, Zhengshan
Zenkoh, Junko
Taniguchi, Hideki
Tsuboi, Koji
In vitro stemness characterization of radio-resistant clones isolated from a medulloblastoma cell line ONS-76
title In vitro stemness characterization of radio-resistant clones isolated from a medulloblastoma cell line ONS-76
title_full In vitro stemness characterization of radio-resistant clones isolated from a medulloblastoma cell line ONS-76
title_fullStr In vitro stemness characterization of radio-resistant clones isolated from a medulloblastoma cell line ONS-76
title_full_unstemmed In vitro stemness characterization of radio-resistant clones isolated from a medulloblastoma cell line ONS-76
title_short In vitro stemness characterization of radio-resistant clones isolated from a medulloblastoma cell line ONS-76
title_sort in vitro stemness characterization of radio-resistant clones isolated from a medulloblastoma cell line ons-76
topic Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3534279/
https://www.ncbi.nlm.nih.gov/pubmed/22951319
http://dx.doi.org/10.1093/jrr/rrs078
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