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In vitro stemness characterization of radio-resistant clones isolated from a medulloblastoma cell line ONS-76
One-third of patients with medulloblastoma die due to recurrence after various treatments including radiotherapy. Although it has been postulated that cancer stem-like cells are radio-resistant and play an important role in tumor recurrence, the “stemness” of medulloblastoma cells surviving irradiat...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3534279/ https://www.ncbi.nlm.nih.gov/pubmed/22951319 http://dx.doi.org/10.1093/jrr/rrs078 |
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author | Sun, Lue Moritake, Takashi Zheng, Yun-Wen Suzuki, Kenshi Gerelchuluun, Ariungerel Hong, Zhengshan Zenkoh, Junko Taniguchi, Hideki Tsuboi, Koji |
author_facet | Sun, Lue Moritake, Takashi Zheng, Yun-Wen Suzuki, Kenshi Gerelchuluun, Ariungerel Hong, Zhengshan Zenkoh, Junko Taniguchi, Hideki Tsuboi, Koji |
author_sort | Sun, Lue |
collection | PubMed |
description | One-third of patients with medulloblastoma die due to recurrence after various treatments including radiotherapy. Although it has been postulated that cancer stem-like cells are radio-resistant and play an important role in tumor recurrence, the “stemness” of medulloblastoma cells surviving irradiation has not yet been elucidated. Using a medulloblastoma cell line ONS-76, cells that survived gamma irradiation were investigated on their “stemness” in vitro. From 10 500 cells, 20 radio-resistant clones were selected after gamma ray irradiation (5 Gy × two fractions) using the replica micro-well technique. These 20 resistant clones were screened for CD133 positivity by flow cytometry followed by side population assay, tumor sphere formation assay and clonogenic survival assay. Results revealed CD133 fractions were significantly elevated in three clones, which also exhibited significantly increased levels of tumor sphere formation ability and side population fraction. Clonogenic survival assay demonstrated that their radio-resistance was significantly higher than the parental ONS-76. This may support the hypothesis that a small number of cancer stem-like cells (CSCs) are the main culprits in local recurrence after radiotherapy, and disruption of the resistance mechanism of these CSCs is a critical future issue in improving the outcome of patients with medulloblastoma. |
format | Online Article Text |
id | pubmed-3534279 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-35342792013-01-03 In vitro stemness characterization of radio-resistant clones isolated from a medulloblastoma cell line ONS-76 Sun, Lue Moritake, Takashi Zheng, Yun-Wen Suzuki, Kenshi Gerelchuluun, Ariungerel Hong, Zhengshan Zenkoh, Junko Taniguchi, Hideki Tsuboi, Koji J Radiat Res Biology One-third of patients with medulloblastoma die due to recurrence after various treatments including radiotherapy. Although it has been postulated that cancer stem-like cells are radio-resistant and play an important role in tumor recurrence, the “stemness” of medulloblastoma cells surviving irradiation has not yet been elucidated. Using a medulloblastoma cell line ONS-76, cells that survived gamma irradiation were investigated on their “stemness” in vitro. From 10 500 cells, 20 radio-resistant clones were selected after gamma ray irradiation (5 Gy × two fractions) using the replica micro-well technique. These 20 resistant clones were screened for CD133 positivity by flow cytometry followed by side population assay, tumor sphere formation assay and clonogenic survival assay. Results revealed CD133 fractions were significantly elevated in three clones, which also exhibited significantly increased levels of tumor sphere formation ability and side population fraction. Clonogenic survival assay demonstrated that their radio-resistance was significantly higher than the parental ONS-76. This may support the hypothesis that a small number of cancer stem-like cells (CSCs) are the main culprits in local recurrence after radiotherapy, and disruption of the resistance mechanism of these CSCs is a critical future issue in improving the outcome of patients with medulloblastoma. Oxford University Press 2013-01 2012-09-05 /pmc/articles/PMC3534279/ /pubmed/22951319 http://dx.doi.org/10.1093/jrr/rrs078 Text en © The Author 2012. Published by Oxford University Press on behalf of The Japan Radiation Research Society and Japanese Society for Therapeutic Radiology and Oncology. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Biology Sun, Lue Moritake, Takashi Zheng, Yun-Wen Suzuki, Kenshi Gerelchuluun, Ariungerel Hong, Zhengshan Zenkoh, Junko Taniguchi, Hideki Tsuboi, Koji In vitro stemness characterization of radio-resistant clones isolated from a medulloblastoma cell line ONS-76 |
title | In vitro stemness characterization of radio-resistant clones isolated from a medulloblastoma cell line ONS-76 |
title_full | In vitro stemness characterization of radio-resistant clones isolated from a medulloblastoma cell line ONS-76 |
title_fullStr | In vitro stemness characterization of radio-resistant clones isolated from a medulloblastoma cell line ONS-76 |
title_full_unstemmed | In vitro stemness characterization of radio-resistant clones isolated from a medulloblastoma cell line ONS-76 |
title_short | In vitro stemness characterization of radio-resistant clones isolated from a medulloblastoma cell line ONS-76 |
title_sort | in vitro stemness characterization of radio-resistant clones isolated from a medulloblastoma cell line ons-76 |
topic | Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3534279/ https://www.ncbi.nlm.nih.gov/pubmed/22951319 http://dx.doi.org/10.1093/jrr/rrs078 |
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