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Unique immunologic patterns in fibromyalgia

BACKGROUND: Fibromyalgia (FM) is a clinical syndrome characterized by chronic pain and allodynia. The diagnosis of FM has been one of exclusion as a test to confirm the diagnosis is lacking. Recent data highlight the role of the immune system in FM. Aberrant expressions of immune mediators, such as...

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Autores principales: Behm, Frederick G, Gavin, Igor M, Karpenko, Oleksiy, Lindgren, Valerie, Gaitonde, Sujata, Gashkoff, Peter A, Gillis, Bruce S
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3534336/
https://www.ncbi.nlm.nih.gov/pubmed/23245186
http://dx.doi.org/10.1186/1472-6890-12-25
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author Behm, Frederick G
Gavin, Igor M
Karpenko, Oleksiy
Lindgren, Valerie
Gaitonde, Sujata
Gashkoff, Peter A
Gillis, Bruce S
author_facet Behm, Frederick G
Gavin, Igor M
Karpenko, Oleksiy
Lindgren, Valerie
Gaitonde, Sujata
Gashkoff, Peter A
Gillis, Bruce S
author_sort Behm, Frederick G
collection PubMed
description BACKGROUND: Fibromyalgia (FM) is a clinical syndrome characterized by chronic pain and allodynia. The diagnosis of FM has been one of exclusion as a test to confirm the diagnosis is lacking. Recent data highlight the role of the immune system in FM. Aberrant expressions of immune mediators, such as cytokines, have been linked to the pathogenesis and traits of FM. We therefore determined whether cytokine production by immune cells is altered in FM patients by comparing the cellular responses to mitogenic activators of stimulated blood mononuclear cells of a large number of patients with FM to those of healthy matched individuals. METHODS: Plasma and peripheral blood mononuclear cells (PBMC) were collected from 110 patients with the clinical diagnosis of FM and 91 healthy donors. Parallel samples of PBMC were cultured overnight in medium alone or in the presence of mitogenic activators; PHA or PMA in combination with ionomycin. The cytokine concentrations of IFN-γ, IL-5, IL-6, IL-8, IL-10, MIP-1β , MCP-1, and MIP1-α in plasma as well as in cultured supernatants were determined using a multiplex immunoassay using bead array technology. RESULTS: Cytokine levels of stimulated PBMC cultures of healthy control subjects were significantly increased as compared to matched non-stimulated PBMC cultures. In contrast, the concentrations of most cytokines were lower in stimulated samples from patients with FM compared to controls. The decreases of cytokine concentrations in patients samples ranged from 1.5-fold for MIP-1β to 10.2-fold for IL-6 in PHA challenges. In PMA challenges, we observed 1.8 to 4-fold decreases in the concentrations of cytokines in patient samples. CONCLUSION: The cytokine responses to mitogenic activators of PBMC isolated from patients with FM were significantly lower than those of healthy individuals, implying that cell-mediated immunity is impaired in FM patients. This novel cytokine assay reveals unique and valuable immunologic traits, which, when combined with clinical patterns, can offer a diagnostic methodology in FM.
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spelling pubmed-35343362013-01-03 Unique immunologic patterns in fibromyalgia Behm, Frederick G Gavin, Igor M Karpenko, Oleksiy Lindgren, Valerie Gaitonde, Sujata Gashkoff, Peter A Gillis, Bruce S BMC Clin Pathol Research Article BACKGROUND: Fibromyalgia (FM) is a clinical syndrome characterized by chronic pain and allodynia. The diagnosis of FM has been one of exclusion as a test to confirm the diagnosis is lacking. Recent data highlight the role of the immune system in FM. Aberrant expressions of immune mediators, such as cytokines, have been linked to the pathogenesis and traits of FM. We therefore determined whether cytokine production by immune cells is altered in FM patients by comparing the cellular responses to mitogenic activators of stimulated blood mononuclear cells of a large number of patients with FM to those of healthy matched individuals. METHODS: Plasma and peripheral blood mononuclear cells (PBMC) were collected from 110 patients with the clinical diagnosis of FM and 91 healthy donors. Parallel samples of PBMC were cultured overnight in medium alone or in the presence of mitogenic activators; PHA or PMA in combination with ionomycin. The cytokine concentrations of IFN-γ, IL-5, IL-6, IL-8, IL-10, MIP-1β , MCP-1, and MIP1-α in plasma as well as in cultured supernatants were determined using a multiplex immunoassay using bead array technology. RESULTS: Cytokine levels of stimulated PBMC cultures of healthy control subjects were significantly increased as compared to matched non-stimulated PBMC cultures. In contrast, the concentrations of most cytokines were lower in stimulated samples from patients with FM compared to controls. The decreases of cytokine concentrations in patients samples ranged from 1.5-fold for MIP-1β to 10.2-fold for IL-6 in PHA challenges. In PMA challenges, we observed 1.8 to 4-fold decreases in the concentrations of cytokines in patient samples. CONCLUSION: The cytokine responses to mitogenic activators of PBMC isolated from patients with FM were significantly lower than those of healthy individuals, implying that cell-mediated immunity is impaired in FM patients. This novel cytokine assay reveals unique and valuable immunologic traits, which, when combined with clinical patterns, can offer a diagnostic methodology in FM. BioMed Central 2012-12-17 /pmc/articles/PMC3534336/ /pubmed/23245186 http://dx.doi.org/10.1186/1472-6890-12-25 Text en Copyright ©2012 Behm et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Behm, Frederick G
Gavin, Igor M
Karpenko, Oleksiy
Lindgren, Valerie
Gaitonde, Sujata
Gashkoff, Peter A
Gillis, Bruce S
Unique immunologic patterns in fibromyalgia
title Unique immunologic patterns in fibromyalgia
title_full Unique immunologic patterns in fibromyalgia
title_fullStr Unique immunologic patterns in fibromyalgia
title_full_unstemmed Unique immunologic patterns in fibromyalgia
title_short Unique immunologic patterns in fibromyalgia
title_sort unique immunologic patterns in fibromyalgia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3534336/
https://www.ncbi.nlm.nih.gov/pubmed/23245186
http://dx.doi.org/10.1186/1472-6890-12-25
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