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Caffeic Acid Phenethyl Ester Inhibits Oral Cancer Cell Metastasis by Regulating Matrix Metalloproteinase-2 and the Mitogen-Activated Protein Kinase Pathway

Caffeic acid phenethyl ester (CAPE), an active component extracted from honeybee hives, exhibits anti-inflammatory and anticancer activities. However, the molecular mechanism by which CAPE affects oral cancer cell metastasis has yet to be elucidated. In this study, we investigated the potential mech...

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Autores principales: Peng, Chih-Yu, Yang, Hui-Wen, Chu, Yin-Hung, Chang, Yu-Chao, Hsieh, Ming-Ju, Chou, Ming-Yung, Yeh, Kun-Tu, Lin, Yueh-Min, Yang, Shun-Fa, Lin, Chiao-Wen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3535744/
https://www.ncbi.nlm.nih.gov/pubmed/23320037
http://dx.doi.org/10.1155/2012/732578
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author Peng, Chih-Yu
Yang, Hui-Wen
Chu, Yin-Hung
Chang, Yu-Chao
Hsieh, Ming-Ju
Chou, Ming-Yung
Yeh, Kun-Tu
Lin, Yueh-Min
Yang, Shun-Fa
Lin, Chiao-Wen
author_facet Peng, Chih-Yu
Yang, Hui-Wen
Chu, Yin-Hung
Chang, Yu-Chao
Hsieh, Ming-Ju
Chou, Ming-Yung
Yeh, Kun-Tu
Lin, Yueh-Min
Yang, Shun-Fa
Lin, Chiao-Wen
author_sort Peng, Chih-Yu
collection PubMed
description Caffeic acid phenethyl ester (CAPE), an active component extracted from honeybee hives, exhibits anti-inflammatory and anticancer activities. However, the molecular mechanism by which CAPE affects oral cancer cell metastasis has yet to be elucidated. In this study, we investigated the potential mechanisms underlying the effects of CAPE on the invasive ability of SCC-9 oral cancer cells. Results showed that CAPE attenuated SCC-9 cell migration and invasion at noncytotoxic concentrations (0 μM to 40 μM). Western blot and gelatin zymography analysis findings further indicated that CAPE downregulated matrix metalloproteinase-2 (MMP-2) protein expression and inhibited its enzymatic activity. CAPE exerted its inhibitory effects on MMP-2 expression and activity by upregulating tissue inhibitor of metalloproteinase-2 (TIMP-2) and potently decreased migration by reducing focal adhesion kinase (FAK) phosphorylation and the activation of its downstream signaling molecules p38/MAPK and JNK. These data indicate that CAPE could potentially be used as a chemoagent to prevent oral cancer metastasis.
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spelling pubmed-35357442013-01-14 Caffeic Acid Phenethyl Ester Inhibits Oral Cancer Cell Metastasis by Regulating Matrix Metalloproteinase-2 and the Mitogen-Activated Protein Kinase Pathway Peng, Chih-Yu Yang, Hui-Wen Chu, Yin-Hung Chang, Yu-Chao Hsieh, Ming-Ju Chou, Ming-Yung Yeh, Kun-Tu Lin, Yueh-Min Yang, Shun-Fa Lin, Chiao-Wen Evid Based Complement Alternat Med Research Article Caffeic acid phenethyl ester (CAPE), an active component extracted from honeybee hives, exhibits anti-inflammatory and anticancer activities. However, the molecular mechanism by which CAPE affects oral cancer cell metastasis has yet to be elucidated. In this study, we investigated the potential mechanisms underlying the effects of CAPE on the invasive ability of SCC-9 oral cancer cells. Results showed that CAPE attenuated SCC-9 cell migration and invasion at noncytotoxic concentrations (0 μM to 40 μM). Western blot and gelatin zymography analysis findings further indicated that CAPE downregulated matrix metalloproteinase-2 (MMP-2) protein expression and inhibited its enzymatic activity. CAPE exerted its inhibitory effects on MMP-2 expression and activity by upregulating tissue inhibitor of metalloproteinase-2 (TIMP-2) and potently decreased migration by reducing focal adhesion kinase (FAK) phosphorylation and the activation of its downstream signaling molecules p38/MAPK and JNK. These data indicate that CAPE could potentially be used as a chemoagent to prevent oral cancer metastasis. Hindawi Publishing Corporation 2012 2012-12-18 /pmc/articles/PMC3535744/ /pubmed/23320037 http://dx.doi.org/10.1155/2012/732578 Text en Copyright © 2012 Chih-Yu Peng et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Peng, Chih-Yu
Yang, Hui-Wen
Chu, Yin-Hung
Chang, Yu-Chao
Hsieh, Ming-Ju
Chou, Ming-Yung
Yeh, Kun-Tu
Lin, Yueh-Min
Yang, Shun-Fa
Lin, Chiao-Wen
Caffeic Acid Phenethyl Ester Inhibits Oral Cancer Cell Metastasis by Regulating Matrix Metalloproteinase-2 and the Mitogen-Activated Protein Kinase Pathway
title Caffeic Acid Phenethyl Ester Inhibits Oral Cancer Cell Metastasis by Regulating Matrix Metalloproteinase-2 and the Mitogen-Activated Protein Kinase Pathway
title_full Caffeic Acid Phenethyl Ester Inhibits Oral Cancer Cell Metastasis by Regulating Matrix Metalloproteinase-2 and the Mitogen-Activated Protein Kinase Pathway
title_fullStr Caffeic Acid Phenethyl Ester Inhibits Oral Cancer Cell Metastasis by Regulating Matrix Metalloproteinase-2 and the Mitogen-Activated Protein Kinase Pathway
title_full_unstemmed Caffeic Acid Phenethyl Ester Inhibits Oral Cancer Cell Metastasis by Regulating Matrix Metalloproteinase-2 and the Mitogen-Activated Protein Kinase Pathway
title_short Caffeic Acid Phenethyl Ester Inhibits Oral Cancer Cell Metastasis by Regulating Matrix Metalloproteinase-2 and the Mitogen-Activated Protein Kinase Pathway
title_sort caffeic acid phenethyl ester inhibits oral cancer cell metastasis by regulating matrix metalloproteinase-2 and the mitogen-activated protein kinase pathway
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3535744/
https://www.ncbi.nlm.nih.gov/pubmed/23320037
http://dx.doi.org/10.1155/2012/732578
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